72088-94-9
72088-94-9
Chemical Structure
5(6)-Carboxyfluorescein
Synonym(s): 5(6)-FAM; 5-(and-6)-Carboxyfluorescein mixed isomers
- CAS No.: 72088-94-9
- Formula:C42H24O14
- Molecular Weight:752.63
IUPAC Name: 3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid compound with 3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylic acid (1:1)
InChIKey: BPVHBBXCESDRKW-UHFFFAOYSA-N
SMILES: OC1=CC=C(C2(O3)C(C=C(C(O)=O)C=C4)=C4C3=O)C(OC5=C2C=CC(O)=C5)=C1.OC6=CC=C(C7(O8)C(C=CC(C(O)=O)=C9)=C9C8=O)C(OC%10=C7C=CC(O)=C%10)=C6
Biological Activity: 5(6)-Carboxyfluorescein (5(6)-FAM) is an amine-reactive pH-sensitive green fluorescent probe. 5(6)-Carboxyfluorescein (5(6)-FAM) can be used to label proteins, peptides and nucleotides. 5(6)-Carboxyfluorescein can be used for the detection of tumour areas in vivo[1][2].
| Cat. No. | Product Name | Purity | Description | Pricing | |||||||||||||||||||
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5(6)-Carboxyfluorescein | 99.66% | 5(6)-Carboxyfluorescein (5(6)-FAM) is an amine-reactive pH-sensitive green fluorescent probe. 5(6)-Carboxyfluorescein (5(6)-FAM) can be used to label proteins, peptides and nucleotides. 5(6)-Carboxyfluorescein can be used for the detection of tumour areas in vivo. | ||||||||||||||||||||
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5(6)-FITC | 97.59% | 5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is an amine-reactive derivative of a fluorescent dye, characterized by high absorbance and excellent fluorescence quantum yield. The isothiocyanate group of FITC can react with various functional groups on proteins, including amines, thiols, imidazoles, tyrosines and carbonyls, enabling the labeling of proteins such as antibodies and lectins. 5(6)-FITC has a wide range of applications, including flow cytometry, immunofluorescence, protease assays and conjugation. The maximum excitation/emission wavelengths are 492/518 nm. | ||||||||||||||||||||
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5(6)-TAMRA | 98.51% | 5(6)-TAMRA (5(6)-Carboxytetramethylrhodamine) is a fluorescent dye molecule widely used as a label for peptides and proteins (Ex/Em = 520/600 nm). | ||||||||||||||||||||
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6-FAM | 99.72% | 6-FAM (6-Carboxyfluorescein) is an isomer of carboxyfluorescein and is mainly used for sequencing and labeling of nucleic acids. | ||||||||||||||||||||
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5-FAM | 99.58% | 5-FAM (5-Carboxyfluorescein) is a green fluorescent reagent used for in situ labeling peptides, proteins and nucleotides. 5-FAM is a single isomer with Ex/Em of 490 nm/520 nm. | ||||||||||||||||||||
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5(6)-Aminofluorescein | 98.55% | 5(6)-Aminofluorescein (5(6)-AFM) is an amino-containing fluorescein dye primarily used for biomolecular labeling, fluorescence imaging, and as a precursor for the synthesis of fluorescent probes (Ex/Em = 495/515 nm). | ||||||||||||||||||||
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5(6)-Carboxy-2',7'-dichlorofluorescein | 99.03% | 5 (6)-Carboxy-2',7'-dichlorofluorescein is a fluorescent probe substrate that acts on MRP1 and MRP3/Mrp3, passively diffuses across cell membranes, and is actively effluxed by MRP1 in MRP1-positive cells. 5 (6)-Carboxy-2',7'-dichlorofluorescein is transported across membranes in an ATP-dependent manner, but does not stimulate ATPase activity. | ||||||||||||||||||||
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5(6)-CFDA | 98.75% | 5(6)-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase. CF containing portion contains an additional negative charge so that it is better retained in cells, compared to fluorescein dyes. | ||||||||||||||||||||
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5(6)-Carboxy-2',7'-dichlorofluorescein diacetate | 97.5% | 5 (6)-Carboxy-2',7'-dichlorofluorescein diacetate is a fluorescein-based reactive oxygen species (ROS) probe and also a MRP2 substrate. 5 (6)-Carboxy-2',7'-dichlorofluorescein diacetate serves as a substrate for intracellular esterases, which cleave its acetate groups to generate a fluorescent product capable of detecting intracellular ROS. 5 (6)-Carboxy-2',7'-dichlorofluorescein diacetate is ATP-dependent and is transported via a single MRP2 binding site; it competes with LTC4 for MRP2 binding sites and inhibits MRP2-mediated LTC4 transport (Ex/Em = 496/525 nm). | ||||||||||||||||||||
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5(6)-Carboxyfluorescein diisobutyrate | 5(6)-Carboxyfluorescein diisobutyrate is a protected 5(6)-carboxyfluorescein. 5(6)-carboxyfluorescein is a fluorescence pH indicator. | |||||||||||||||||||||
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BCECF (solution) | BCECF (solution) (2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein (solution)) is a cell-impermeant pH-sensitive fluorescent dye. BCECF allows measurements in the physiological pH range 6.0-8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm. Solvent and concentration: DMSO: 10 mM |
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References
- [1]. Maksim V Kvach, et al. 5(6)-carboxyfluorescein revisited: new protecting group, separation of isomers, and their spectral properties on oligonucleotides. Bioconjug Chem. Sep-Oct 2007;18(5):1691-6. [Content Brief]
- [2]. Wen Ma, et al. A Cell Membrane-Targeting Self-Delivery Chimeric Peptide for Enhanced Photodynamic Therapy and In Situ Therapeutic Feedback. Adv Healthc Mater. 2020 Jan;9(1):e1901100. [Content Brief]
