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  3. 6-FAM

6-FAM  (Synonyms: 6-Carboxyfluorescein)

Cat. No.: HY-66021 Purity: 99.88%
COA Handling Instructions

6-FAM (6-Carboxyfluorescein) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid.

For research use only. We do not sell to patients.

6-FAM Chemical Structure

6-FAM Chemical Structure

CAS No. : 3301-79-9

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply Now  
Solution
10 mM * 1 mL in DMSO USD 101 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 101 In-stock
Estimated Time of Arrival: December 31
Solid
100 mg USD 92 In-stock
Estimated Time of Arrival: December 31
500 mg USD 251 In-stock
Estimated Time of Arrival: December 31
1 g   Get quote  
5 g   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 6-FAM:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

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Description

6-FAM (6-Carboxyfluorescein) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility, fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.

Molecular Weight

376.32

Formula

C21H12O7

CAS No.
SMILES

O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=CC(C(O)=O)=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 40 mg/mL (106.29 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6573 mL 13.2866 mL 26.5731 mL
5 mM 0.5315 mL 2.6573 mL 5.3146 mL
10 mM 0.2657 mL 1.3287 mL 2.6573 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (6.64 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (6.64 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.88%

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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This equation is commonly abbreviated as: C1V1 = C2V2

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Product Name:
6-FAM
Cat. No.:
HY-66021
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