1. Dye Reagents

6-FAM (Synonyms: 6-Carboxyfluorescein)

Cat. No.: HY-66021 Purity: 99.19%
Handling Instructions

The single isomer, 6-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid.

For research use only. We do not sell to patients.

6-FAM Chemical Structure

6-FAM Chemical Structure

CAS No. : 3301-79-9

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 92 In-stock
Estimated Time of Arrival: December 31
100 mg USD 84 In-stock
Estimated Time of Arrival: December 31
500 mg USD 228 In-stock
Estimated Time of Arrival: December 31
1 g   Get quote  
5 g   Get quote  

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Other Forms of 6-FAM:

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Description

The single isomer, 6-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility, fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.

Solvent & Solubility
In Vitro: 

DMSO : ≥ 40 mg/mL (106.29 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6573 mL 13.2866 mL 26.5731 mL
5 mM 0.5315 mL 2.6573 mL 5.3146 mL
10 mM 0.2657 mL 1.3287 mL 2.6573 mL
*Please refer to the solubility information to select the appropriate solvent.
Molecular Weight

376.32

Formula

C₂₁H₁₂O₇

CAS No.

3301-79-9

SMILES

O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=CC(C(O)=O)=C5

Storage

-20°C, protect from light, stored under nitrogen

Shipping

Room temperature in continental US; may vary elsewhere

Purity: 99.19%

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
6-FAM
Cat. No.:
HY-66021
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6-FAM

Cat. No.: HY-66021