4-Di-1-ASP
Based on 1 Customer Validation
4-Di-1-ASP is a styryl dye used to stain glioma cells in living brain tissue for analysis of cell structure, viability, proliferation and endocytosis, cytokinesis and phagocytosis, as well as for observation of mitochondrial structures in living cells. 4-Di-1-ASP fluoresces green when imaged microscopically (λex /λem = 475/606 nm) .
For research use only. We do not sell to patients.
- Purity: 99.95%
- CAS No.: 959-81-9
- Formula: C16H19IN2
- Molecular Weight:366.24
-
Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Dissolve the dye in water and configure a mother liquor at a concentration of 2 mM.
2. Place the prepared cell sections in an incubator at 35°C, add the prepared dye solution to a final concentration of 1 μM and incubate with the stain for 10 min.
3. Observe with a confocal microscope, with a maximum excitation wavelength of 475 nM and a maximum emission wavelength of 606 nM.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
521
488
Chemical Information
-
CAS No. 959-81-9
-
Appearance Solid
-
Molecular Weight 366.24
-
Formula C16H19IN2
-
Color Purple to purplish red
-
SMILES
C[N+]1=CC=C(/C=C/C2=CC=C(N(C)C)C=C2)C=C1.[I-]
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 43.75 mg/mL (119.46 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (271 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. Lilia Y Kucheryavykh, et al. Visualization of implanted GL261 glioma cells in living mouse brain slices using fluorescent 4-(4-(dimethylamino)-styryl)-N-methylpyridinium iodide (ASP+). Biotechniques. 2012 Nov;53(5):305-9. [Content Brief]
[2]. Jensen J H Wong, et al. Simultaneous High-Throughput Conformational and Colloidal Stability Screening Using a Fluorescent Molecular Rotor Dye, 4-(4-(Dimethylamino)styryl)-N-Methylpyridinium Iodide (DASPMI). J Biomol Screen. 2016 Sep;21(8):842-50. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7305 mL | 13.6523 mL | 27.3045 mL | 68.2613 mL |
| 5 mM | 0.5461 mL | 2.7305 mL | 5.4609 mL | 13.6523 mL | |
| 10 mM | 0.2730 mL | 1.3652 mL | 2.7305 mL | 6.8261 mL | |
| 15 mM | 0.1820 mL | 0.9102 mL | 1.8203 mL | 4.5508 mL | |
| 20 mM | 0.1365 mL | 0.6826 mL | 1.3652 mL | 3.4131 mL | |
| 25 mM | 0.1092 mL | 0.5461 mL | 1.0922 mL | 2.7305 mL | |
| 30 mM | 0.0910 mL | 0.4551 mL | 0.9102 mL | 2.2754 mL | |
| 40 mM | 0.0683 mL | 0.3413 mL | 0.6826 mL | 1.7065 mL | |
| 50 mM | 0.0546 mL | 0.2730 mL | 0.5461 mL | 1.3652 mL | |
| 60 mM | 0.0455 mL | 0.2275 mL | 0.4551 mL | 1.1377 mL | |
| 80 mM | 0.0341 mL | 0.1707 mL | 0.3413 mL | 0.8533 mL | |
| 100 mM | 0.0273 mL | 0.1365 mL | 0.2730 mL | 0.6826 mL |
4-Di-1-ASP Related Classifications
HY-W094758A Related Classifications
Powered by Bioz