Cholesterol (from animal)
Based on 85 publication(s) in Google Scholar
Cholesterol (from animal) is the major sterol in mammals. It is making up 20-25% of structural component of the plasma membrane. Plasma membranes are highly permeable to water but relatively impermeable to ions and protons. Cholesterol (from animal) plays an important role in determining the fluidity and permeability characteristics of the membrane as well as the function of both the transporters and signaling proteins. Cholesterol (from animal) is also an endogenous estrogen-related receptor α (ERRα) agonist.
For research use only. We do not sell to patients.
- Purity: 99.94%
- CAS No.: 57-88-5
- Formula: C27H46O
- Molecular Weight:386.65
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
Publications Citing Use of MedChemExpress (MCE) Cholesterol (from animal)
More- Signal Transduct Target Ther. 2025 Mar 24;10(1):101. [Abstract]
- Nature. 2025 Jul;643(8070):192-200. [Abstract]
- Nat Nanotechnol. 2021 Oct;16(10):1150-1160. [Abstract]
- Mol Cancer. 2025 Jul 19;24(1):199. [Abstract]
- Mol Cancer. 2025 May 26;24(1):151. [Abstract]
- Cell Metab. 2024 Nov 5;36(11):2402-2418.e10. [Abstract]
- Nat Biomed Eng. 2025 Feb;9(2):215-233. [Abstract]
- Immunity. 2024 May 14;57(5):1087-1104.e7. [Abstract]
- Cancer Commun (Lond). 2025 Aug;45(8):1010-1037. [Abstract]
- Nat Aging. 2025 Aug 26. [Abstract]
- Cell Host Microbe. 2025 Dec 30:S1931-3128(25)00521-9. [Abstract]
- Nat Commun. 2024 Jan 2;15(1):162. [Abstract]
- Adv Sci (Weinh). 2026 Apr;13(24):e15606. [Abstract]
- Adv Sci (Weinh). 2025 Jun 20:e04195. [Abstract]
- Adv Sci (Weinh). 2024 Aug 9:e2402329. [Abstract]
- Adv Sci (Weinh). 2024 Jul 1:e2403640. [Abstract]
- Adv Sci (Weinh). 2023 Sep;10(27):e2206878. [Abstract]
- Nat Chem Biol. 2022 Nov;18(11):1196-1203. [Abstract]
- Theranostics. 2021 Jan 1;11(2):841-860. [Abstract]
- Exp Mol Med. 2025 May;57(5):1089-1105. [Abstract]
- J Nanobiotechnology. 2022 Oct 20;20(1):454. [Abstract]
- Redox Biol. 2025 Jul:84:103693. [Abstract]
- Redox Biol. 2023 Jun:62:102678. [Abstract]
- J Control Release. 2026 Mar 14:114825. [Abstract]
- J Control Release. 2025 May 30:113907. [Abstract]
- Cell Rep Med. 2026 Feb 17;7(2):102591. [Abstract]
- Cell Rep Med. 2026 Feb 17;7(2):102580. [Abstract]
- Mater Today Bio. 2026 Mar 2:37:102990. [Abstract]
- Nat Struct Mol Biol. 2025 May;32(5):896-904. [Abstract]
- Adv Healthc Mater. 2026 Feb 21:e05684. [Abstract]
- Sci China Life Sci. 2022 Feb;65(2):341-361. [Abstract]
- Small Methods. 2025 Apr 21:e2401712. [Abstract]
- Proc Natl Acad Sci U S A. 2026 Jul 7;123(27):e2523789123.
- Phytomedicine. 2025 May 24:143:156904. [Abstract]
- EMBO Mol Med. 2024 Aug;16(8):1755-1790. [Abstract]
- Phytomedicine. 2024 Mar:125:155299. [Abstract]
- ACS Appl Mater Interfaces. 2025 Oct 8;17(40):55861-55875. [Abstract]
- Free Radic Biol Med. 2024 Aug 27:S0891-5849(24)00623-3. [Abstract]
- ACS Appl Mater Interfaces. 2024 Sep 4;16(35):46044-46052. [Abstract]
- Cell Death Discov. 2025 Feb 8;11(1):55. [Abstract]
- Cell Death Discov. 2024 May 29;10(1):263. [Abstract]
- Int J Nanomedicine. 2026 Mar 20:21:577606. [Abstract]
- Int J Nanomedicine. 2025 Oct 22:20:12783-12804. [Abstract]
- NPJ Vaccines. 2025 Aug 14;10(1):193. [Abstract]
- Cell Biosci. 2025 Jul 4;15(1):95. [Abstract]
- Cell Mol Life Sci. 2024 Jul 6;81(1):289. [Abstract]
- Polym Test. 2023 Dec 1, 108292.
- Plant J. 2025 Nov;124(3):e70559. [Abstract]
- Biochem Pharmacol. 2025 Aug 8;242(Pt 3):117223. [Abstract]
- Biomacromolecules. 2025 Jun 9;26(6):3563-3575. [Abstract]
- J Ethnopharmacol. 2025 Apr 24:119877. [Abstract]
- Int J Pharm. 2024 Aug 18:124608. [Abstract]
- Commun Biol. 2026 Feb 7;9(1):373. [Abstract]
- Life Sci. 2025 Jun 1:370:123571. [Abstract]
- Commun Biol. 2024 Nov 9;7(1):1476. [Abstract]
- Life Sci. 2023 Aug 15:327:121698. [Abstract]
- Respir Res. 2024 Nov 7;25(1):399. [Abstract]
- Bioorg Chem. 2026 Apr:171:109526. [Abstract]
- Nanomedicine. 2024 Jun:58:102745. [Abstract]
- iScience. 2024 Apr 6;27(5):109676. [Abstract]
- Antiviral Res. 2023 Jan:209:105497. [Abstract]
- Sci Rep. 2025 May 26;15(1):18364. [Abstract]
- J Pharmacol Exp Ther. 2025 Dec 24.
- J Pharm Sci. 2024 May 25:S0022-3549(24)00199-0. [Abstract]
- Insect Biochem Mol Biol. 2026 Mar:188:104499. [Abstract]
- Cell Signal. 2024 Sep 16:111419. [Abstract]
- Heliyon. 2026 Jan 22;12(2):e44534.
- Viruses. 2022 Mar 3;14(3):514. [Abstract]
- Pathol Res Pract. 2025 Sep:273:156138. [Abstract]
- Part Part Syst Charact. 2024 Jun 12.
- Life Sci Alliance. 2022 Jan 4;5(3):e202101256. [Abstract]
- Vet Microbiol. 2024 Jan:288:109948. [Abstract]
- Biochem Biophys Res Commun. 2020 Feb 19;522(4):862-868. [Abstract]
- Lett Drug Des Discov. 2026 Feb 11.
- Part Sci Technol. 2025 Jul 03.
- Gene Rep. 2026 Jan 12;42:102429.
- SSRN. 2025 Oct 29.
- Patent. US20250248939A1.
- SSRN. 2025 Aug 29.
- Res Sq. 2025 Jul 29.
- Cell Biomater. 2025 Aug 19.
- Traditional Medicine Research. 2025;10(4):20.
- SSRN. 2024 Feb 5.
- Patent. US20200376146A1.
- Patent. US20200376102A1.
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Cell Imaging/Staining
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Bio/Physico-chemical Assay
All Endogenous Metabolite Isoforms
More
Biological Activity
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Microbial Metabolite |
Human Endogenous Metabolite |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
>20 μM
Compound: b1
|
Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
|
[PMID: 30822712] |
| BJ | IC50 |
>50 μM
Compound: Cholesterol
|
Cytotoxicity against human BJ cells after 72 hrs by calcein AM assay
Cytotoxicity against human BJ cells after 72 hrs by calcein AM assay
|
[PMID: 22417637] |
| CCRF-CEM | IC50 |
>50 μM
Compound: Cholesterol
|
Cytotoxicity against human CEM cells after 72 hrs by calcein AM assay
Cytotoxicity against human CEM cells after 72 hrs by calcein AM assay
|
[PMID: 22417637] |
| HeLa | IC50 |
>50 μM
Compound: Cholesterol
|
Cytotoxicity against human HeLa cells after 72 hrs by calcein AM assay
Cytotoxicity against human HeLa cells after 72 hrs by calcein AM assay
|
[PMID: 22417637] |
| HL-60 | IC50 |
>20 μM
Compound: b1
|
Antiproliferative activity against human HL60 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
Antiproliferative activity against human HL60 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
|
[PMID: 30822712] |
| HT-29 | IC50 |
>50 μM
Compound: 1
|
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar Blue assay
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar Blue assay
|
[PMID: 19473028] |
| MCF7 | IC50 |
>20 μM
Compound: b1
|
Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
|
[PMID: 30822712] |
| MCF7 | IC50 |
>50 μM
Compound: Cholesterol
|
Cytotoxicity against human MCF7 cells after 72 hrs by calcein AM assay
Cytotoxicity against human MCF7 cells after 72 hrs by calcein AM assay
|
[PMID: 22417637] |
| SMMC-7721 | IC50 |
>20 μM
Compound: b1
|
Antiproliferative activity against human SMMC7721 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
Antiproliferative activity against human SMMC7721 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
|
[PMID: 30822712] |
| SW480 | IC50 |
>20 μM
Compound: b1
|
Antiproliferative activity against human SW480 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
Antiproliferative activity against human SW480 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
|
[PMID: 30822712] |
| Vero | IC50 |
>128 μg/mL
Compound: 6
|
Cytotoxicity against african green monkey Vero cells
Cytotoxicity against african green monkey Vero cells
|
[PMID: 18818073] |
GT1-7 hypothalamic cells subjected to Cholesterol depletion in vitro produced 20-31% reductions in cellular Cholesterol content. All Cholesterol-depleted neuron-derived cells, exhibit decreased phosphorylation/activation of IRS-1 and AKT following stimulation by insulin, insulin-like growth factor-1, or the neurotrophins (NGF and BDNF). Reduction in cellular Cholesterol also results in increased basal autophagy and impairment of induction of autophagy by glucose deprivation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Administration: 2% cholesterol; diet • 8 weeks
(2) Wistar rats were always chosen for hyperlipidemia studies since this species shows a moderate increase in serum cholesterol and triglyceride level due to a high-cholesterol diet and no substantial atherosclerosis develops; therefore, the direct myocardial effect of hyperlipidemia, independent from atherosclerosis, can be studied in this model.
Administration: 0.3% cholesterol and 3% soybean oil; diet • 16 weeks
(2) As the absorption of dietary cholesterol requires fat, you must add oil into the diet. Otherwise, rabbits will use their internal fat, which makes them lean or sick. In addition, using soybean oil, which consists of unsaturated fatty acids, can prevent the levels of plasma cholesterol from becoming too high. Other vegetable oils, such as peanut oil or corn oil, can be used because they are all unsaturated fatty acids. Animal fat (saturated fatty acids) like tallow and lard is not recommended.
(3) 0.3-0.5% cholesterol diet is recommended for most experiments. Rabbits cannot tolerate a 1-2% cholesterol diet for a month as they develop severe liver dysfunction.
(4) Adult rabbits at 4 months or older can consume approximately ~150 g a day. You can either feed ab libitum or restricted (100-150 g/day/adult rabbit).
(5) Plasma lipids should be measured weekly, especially for the first 4 weeks, because you need to determine whether plasma levels of cholesterol are elevated in each animal. Non-responder rabbits can be excluded from the experiments if their plasma cholesterol levels do not increase after cholesterol diet feeding.
(6) Plasma lipoproteins can be measured at 8 and 16 weeks when the plasma levels of cholesterol are stable.
(7) The age of rabbits should be considered because young rabbits are more susceptible to aortic atherosclerosis than old rabbits even though they have similar plasma cholesterol levels. 4-6-month-old rabbits are usually used for cholesterol feeding experiments.
(8) Male and female rabbits are different in terms of response to a cholesterol diet and atherosclerosis. In our experience, female rabbits develop higher hypercholesterolemia and greater aortic lesions than their counterpart male rabbits. In general, male rabbits are recommended for experiments because estrogen may influence the results.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 57-88-5
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Appearance Solid
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Molecular Weight 386.65
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Formula C27H46O
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Color White to off-white
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SMILES
O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@@H](CCCC(C)C)C
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (85)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Intranasal prime-boost RNA vaccination elicits potent T cell response for lung cancer therapy. [Abstract]2025 Mar 24;10(1):101. PMID: 40122855 -
Nature
2025 Jul;643(8070):192-200. PMID: 39695227 -
Nat Nanotechnol
Intrinsic bioactivity of black phosphorus nanomaterials on mitotic centrosome destabilization through suppression of PLK1 kinase. [Abstract]2021 Oct;16(10):1150-1160. PMID: 34354264 -
Mol Cancer
CircABCA1 promotes ccRCC by reprogramming cholesterol metabolism and facilitating M2 macrophage polarization through IGF2BP3-mediated stabilization of SCARB1 mRNA. [Abstract]2025 Jul 19;24(1):199. PMID: 40684174 -
Mol Cancer
GP73-dependent regulation of exosome biogenesis promotes colorectal cancer liver metastasis. [Abstract]2025 May 26;24(1):151. PMID: 40414849
Cholesterol (from animal) purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2025 May 26;24(1):151. [Abstract]
Analysis of interactions between GP73 and Cholesterol (0.1-10000 nM, 3 h), 25-HC, 27-HC, campesterol, and epicholesterol by MST.
Cholesterol (from animal) purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2025 May 26;24(1):151. [Abstract]
Analysis of interactions between Cholesterol (0.1-1000 nM, 3 h) and mouse GP73 (WT), the GP73 CRAC domain deletion mutant (∆CRAC) and the CARC Phe90/CRAC Tyr99 double mutant of mouse GP73 (F90I-Y99I, DM) by MST.
Cholesterol (from animal) purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2025 May 26;24(1):151. [Abstract]
Titration curves showing changes in the intrinsic fluorescence of WT, Y99I, F90I-Y99I (DM), or scrambled peptides (5 µM) in the presence of increasing concentrations of Cholesterol (0-140 μM).
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Cell Metab
Sensing steroid hormone 17α-hydroxypregnenolone by GPR56 enables protection from ferroptosis-induced liver injury. [Abstract]2024 Nov 5;36(11):2402-2418.e10. PMID: 39389061 -
Nat Biomed Eng
Potent prophylactic cancer vaccines harnessing surface antigens shared by tumour cells and induced pluripotent stem cells. [Abstract]2025 Feb;9(2):215-233. PMID: 39730914 -
Immunity
25-Hydroxycholesterol regulates lysosome AMP kinase activation and metabolic reprogramming to educate immunosuppressive macrophages. [Abstract]2024 May 14;57(5):1087-1104.e7. PMID: 38640930 -
Cancer Commun (Lond)
Lipid metabolism reprograming by SREBP1-PCSK9 targeting sensitizes pancreatic cancer to immunochemotherapy. [Abstract]2025 Aug;45(8):1010-1037. PMID: 40439109 -
Nat Aging
2025 Aug 26. PMID: 40858817 -
Cell Host Microbe
2025 Dec 30:S1931-3128(25)00521-9. PMID: 41475336 -
Nat Commun
Tubeimosides are pan-coronavirus and filovirus inhibitors that can block their fusion protein binding to Niemann-Pick C1. [Abstract]2024 Jan 2;15(1):162. PMID: 38167417
Cholesterol (from animal) purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jan 2;15(1):162. [Abstract]
Detection of the exogenous cholesterol activity in SARS2 infection. Indicated Caco2 cells were infected with SARS2-Spseudoviruses and treated with cholesterol (0-200 μg/mL) at indicated concentrations. After 48 hours, viral infection was determined by measuring the intracellular luciferase activity. RLU, relative light unit.
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Adv Sci (Weinh)
An Insulin-Exosome-TNFAIP8 Axis Drives Stromal Fibrosis and Therapeutic Resistance in Pancreatic Cancer. [Abstract]2026 Apr;13(24):e15606. PMID: 41709860 -
Adv Sci (Weinh)
Brachyury-Activated Fucoidan Hydrogel Microspheres Rejuvenate Degenerative Intervertebral Discs Microenvironment. [Abstract]2025 Jun 20:e04195. PMID: 40539715
Cholesterol (from animal) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jun 20:e04195. [Abstract]
Dil staining of GF and BLNP (the formulation (50:38.5:10:1.5) included ionizable lipids (D‐Lin‐MC3‐DMA), Cholesterol, DSPC, and DMG‐PEG2000‐NH2, which were homogeneously mixed in the ethanol phase (12 mM))@GF microspheres (scale bar = 50 µm).
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Adv Sci (Weinh)
CircUGP2 Suppresses Intrahepatic Cholangiocarcinoma Progression via p53 Signaling Through Interacting With PURB to Regulate ADGRB1 Transcription and Sponging miR-3191-5p. [Abstract]2024 Aug 9:e2402329. PMID: 39120980 -
Adv Sci (Weinh)
Granulosa Cell-Layer Stiffening Prevents Escape of Mural Granulosa Cells from the Post-Ovulatory Follicle. [Abstract]2024 Jul 1:e2403640. PMID: 38946588 -
Adv Sci (Weinh)
Targeting Squalene Epoxidase Confers Metabolic Vulnerability and Overcomes Chemoresistance in HNSCC. [Abstract]2023 Sep;10(27):e2206878. PMID: 37490552
Cholesterol (from animal) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Sep;10(27):e2206878. [Abstract]
Effects of cholesterol repletion (20 μg/mL; 4 days) on colony formation potential in SCC-1cisR and SCC-23cisR cells with SQLE depletion in the presence of cisplatin (n = 4) were assessed.
Cholesterol (from animal) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Sep;10(27):e2206878. [Abstract]
SCC-1cisR and SCC-23cisR cells transfected with siCTRL or siSQLE were incubated in medium with or without 20 μg/mL Cholesterol for 24 h.
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Nat Chem Biol
2022 Nov;18(11):1196-1203. PMID: 35982227 -
Theranostics
ACSS3 represses prostate cancer progression through downregulating lipid droplet-associated protein PLIN3. [Abstract]2021 Jan 1;11(2):841-860. PMID: 33391508 -
Exp Mol Med
Cholesterol biosynthesis induced by radiotherapy inhibits cGAS-STING activation and contributes to colorectal cancer treatment resistance. [Abstract]2025 May;57(5):1089-1105. PMID: 40355720 -
J Nanobiotechnology
Targeted delivery and ROS-responsive release of Resolvin D1 by platelet chimeric liposome ameliorates myocardial ischemia-reperfusion injury. [Abstract]2022 Oct 20;20(1):454. PMID: 36266658 -
Redox Biol
Dysregulated lipids homeostasis disrupts CHAC1-mediated ferroptosis driving fibroblast growth factor receptor tyrosine kinase inhibitor AZD4547 resistance in gastric cancer. [Abstract]2025 Jul:84:103693. PMID: 40460553 -
Redox Biol
Accumulated cholesterol protects tumours from elevated lipid peroxidation in the microenvironment. [Abstract]2023 Jun:62:102678. PMID: 36940607 -
J Control Release
Impact of ionizable lipid source on quality and stability of siRNA-loaded lipid nanoparticles. [Abstract]2026 Mar 14:114825. PMID: 41839265 -
J Control Release
Functional lipid nanoparticles for safe delivery of macromolecular antibiotics to gram-negative bacteria. [Abstract]2025 May 30:113907. PMID: 40451556 -
Cell Rep Med
Synergistic mechanical and therapeutic modulation of engineered tumor cell-derived microparticles for enhanced cancer treatment. [Abstract]2026 Feb 17;7(2):102591. PMID: 41666924 -
Cell Rep Med
Synthetic cleavage-resistant TREM2 boosts macrophage efferocytosis to treat inflammatory diseases. [Abstract]2026 Feb 17;7(2):102580. PMID: 41616764 -
Mater Today Bio
Ultrasound-triggered dissolving microneedle platform for cGAS-STING-mediated precision immunotherapy of melanoma. [Abstract]2026 Mar 2:37:102990. PMID: 41815866 -
Nat Struct Mol Biol
2025 May;32(5):896-904. PMID: 39824976 -
Adv Healthc Mater
An Injectable Liposome-Hydrogel Composite for Targeted Delivery of a Non-Lactylated Peptide to Rebalance Bone Metabolism in Hyperlipidemia. [Abstract]2026 Feb 21:e05684. PMID: 41721628 -
Sci China Life Sci
2022 Feb;65(2):341-361. PMID: 34047913 -
Small Methods
Cholesterol-Derived Mannosylated Polypeptide-Formed Lipid Nanoparticles for Efficient in Vivo mRNA Delivery. [Abstract]2025 Apr 21:e2401712. PMID: 40256901 -
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Phytomedicine
Disruption of cholesterol homeostasis triggers NLRP3-cGAS-STING axis-dependent hepatic fibrosis and honokiol intervention effects. [Abstract]2025 May 24:143:156904. PMID: 40449452 -
EMBO Mol Med
LILRB1-HLA-G axis defines a checkpoint driving natural killer cell exhaustion in tuberculosis. [Abstract]2024 Aug;16(8):1755-1790. PMID: 39030302 -
Phytomedicine
Diosgenin as a substitute for cholesterol alleviates NAFLD by affecting CYP7A1 and NPC1L1-related pathway. [Abstract]2024 Mar:125:155299. PMID: 38301301 -
ACS Appl Mater Interfaces
Feedback-Amplified Drug Delivery of αPD-L1-Modified Lipoplatin for Chronological Synergy in NSCLC through PD-L1 Upregulation. [Abstract]2025 Oct 8;17(40):55861-55875. PMID: 40997865 -
Free Radic Biol Med
25-Hydroxycholesterol inhibits Hantavirus infection by reprogramming cholesterol metabolism. [Abstract]2024 Aug 27:S0891-5849(24)00623-3. PMID: 39209137 -
ACS Appl Mater Interfaces
Size-Controllable and Monodispersed Lipid Nanoparticle Production with High mRNA Delivery Efficiency Using 3D-Printed Ring Micromixers. [Abstract]2024 Sep 4;16(35):46044-46052. PMID: 39103250 -
Cell Death Discov
Inhibition of lanosterol synthase linking with MAPK/JNK signaling pathway suppresses endometrial cancer. [Abstract]2025 Feb 8;11(1):55. PMID: 39922821 -
Cell Death Discov
Upregulation of rate-limiting enzymes in cholesterol metabolism by PKCδ mediates endothelial apoptosis in diabetic wound healing. [Abstract]2024 May 29;10(1):263. PMID: 38811564 -
Int J Nanomedicine
Intraperitoneal Co-Delivery of Claudin18.2×41BB and EpCAM×CD3 Bispecific Antibodies via mRNA-LNPs Synergistically Suppresses Gastric Cancer Peritoneal Metastasis Through T Cell Co-Activation. [Abstract]2026 Mar 20:21:577606. PMID: 41884275 -
Int J Nanomedicine
Optimized Lipid Nanoparticle-Mediated mRNA Co-Delivery of SOX5/SOX9 Enables Synergistic Senescence Reversal for Osteoarthritis Therapy. [Abstract]2025 Oct 22:20:12783-12804. PMID: 41146650 -
NPJ Vaccines
A self-amplifying mRNA vaccine expressing PRV gD induces robust immunity against virulent mutants. [Abstract]2025 Aug 14;10(1):193. PMID: 40813770 -
Cell Biosci
2025 Jul 4;15(1):95. PMID: 40615893 -
Cell Mol Life Sci
Human cytomegalovirus infection impairs neural differentiation via repressing sterol regulatory element binding protein 2-mediated cholesterol biosynthesis. [Abstract]2024 Jul 6;81(1):289. PMID: 38970696 -
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Plant J
Unveiling the inequivalent biochemical functions of OsSMO2-1 and OsSMO2-2 in rice phytosterol biosynthesis using a customized sterol standards mixture. [Abstract]2025 Nov;124(3):e70559. PMID: 41194406 -
Biochem Pharmacol
Triptolide induces the secretion of insulin, through which it alleviates the tauopathies of Alzheimer's disease via inhibiting the phosphorylation of tau. [Abstract]2025 Aug 8;242(Pt 3):117223. PMID: 40784568 -
Biomacromolecules
Replacing PEG-Lipid with Amphiphilic Polycarbonates in mRNA-Loaded Lipid Nanoparticles: Impact of Polycarbonate Structure on Physicochemical and Transfection Properties. [Abstract]2025 Jun 9;26(6):3563-3575. PMID: 40347133 -
J Ethnopharmacol
Tylophora yunnanensis extract inhibits cholesterol biosynthesis to suppress triple negative breast cancer. [Abstract]2025 Apr 24:119877. PMID: 40287115 -
Int J Pharm
Development of a proliposomal pretomanid dry powder inhaler as a novel alternative approach for combating pulmonary tuberculosis. [Abstract]2024 Aug 18:124608. PMID: 39163929 -
Commun Biol
SREBF2 enhances lipid metabolism and represses anti-tumor immune responses in cervical cancer by increasing ACAT2. [Abstract]2026 Feb 7;9(1):373. PMID: 41654690 -
Life Sci
CKN reduces TLR4-mediated inflammation and cerebral I/R injury by activating the LXRα/ABCA1 pathway in microglia. [Abstract]2025 Jun 1:370:123571. PMID: 40107493 -
Commun Biol
Mevalonate pathway inhibition reduces bladder cancer metastasis by modulating RhoB protein stability and integrin β1 localization. [Abstract]2024 Nov 9;7(1):1476. PMID: 39521858 -
Life Sci
Cordycepin reprogramming lipid metabolism to block metastasis and EMT via ERO1A/mTOR/SREBP1 axis in cholangiocarcinoma. [Abstract]2023 Aug 15:327:121698. PMID: 37080351 -
Respir Res
Activation of LXR signaling ameliorates apoptosis of alveolar epithelial cells in Bronchopulmonary dysplasia. [Abstract]2024 Nov 7;25(1):399. PMID: 39511537 -
Bioorg Chem
Design and synthesis of novel ionizable lipids enables highly efficient mRNA delivery via lipid nanoparticles. [Abstract]2026 Apr:171:109526. PMID: 41621179 -
Nanomedicine
Quantitative analysis of mRNA-lipid nanoparticle stability in human plasma and serum by size-exclusion chromatography coupled with dual-angle light scattering. [Abstract]2024 Jun:58:102745. PMID: 38499167 -
iScience
CRISPR-Cas9 screening identifies INTS3 as an anti-apoptotic RNA-binding protein and therapeutic target for colorectal cancer. [Abstract]2024 Apr 6;27(5):109676. PMID: 38665208 -
Antiviral Res
Targeting 7-dehydrocholesterol reductase against EV-A71 replication by upregulating interferon response. [Abstract]2023 Jan:209:105497. PMID: 36528172 -
Sci Rep
Serum starvation-induced cholesterol reduction increases melanoma cell susceptibility to cytotoxic T lymphocyte killing. [Abstract]2025 May 26;15(1):18364. PMID: 40419522 -
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J Pharm Sci
Development of new nanoniosome carriers for vorinostat: Evaluation of anticancer efficacy in vitro. [Abstract]2024 May 25:S0022-3549(24)00199-0. PMID: 38801974 -
Insect Biochem Mol Biol
2026 Mar:188:104499. PMID: 41548698 -
Cell Signal
Activated PARP1/FAK/COL5A1 signaling facilitates the tumorigenesis of cholesterol-resistant ovarian cancer cells through promoting EMT. [Abstract]2024 Sep 16:111419. PMID: 39293744 -
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Viruses
2022 Mar 3;14(3):514. PMID: 35336921 -
Pathol Res Pract
Alendronate-functionalized ZIF-8 nanoplatform for targeted delivery of SHH siRNA and docetaxel to treat prostate cancer bone metastasis. [Abstract]2025 Sep:273:156138. PMID: 40694986 -
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Life Sci Alliance
Necrotic debris and STING exert therapeutically relevant effects on tumor cholesterol homeostasis. [Abstract]2022 Jan 4;5(3):e202101256. PMID: 34983824 -
Vet Microbiol
The activation of liver X receptors in Madin-Darby bovine kidney cells and mice restricts infection by bovine viral diarrhea virus. [Abstract]2024 Jan:288:109948. PMID: 38113573 -
Biochem Biophys Res Commun
Combinatorial screening of a panel of FDA-approved drugs identifies several candidates with anti-Ebola activities. [Abstract]2020 Feb 19;522(4):862-868. PMID: 31806372 -
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Solvent & Solubility
Ethanol : 10 mg/mL (25.86 mM; ultrasonic and warming and heat to 60°C)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.43 mg/mL (3.70 mM); Clear solution
This protocol yields a clear solution of ≥ 1.43 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (14.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 1.43 mg/mL (3.70 mM); Clear solution
This protocol yields a clear solution of ≥ 1.43 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (14.3 mg/mL) to 900 μL Corn oil, and mix evenly.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
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Data Sheet (285 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Casaburi I, et al. Cholesterol as an Endogenous ERRα Agonist: A New Perspective to Cancer Treatment. Front Endocrinol (Lausanne). 2018 Sep 11;9:525. [Content Brief]
[2]. Dietschy JM, et al. Thematic review series: brain Lipids. Cholesterol metabolism in the central nervous system during early development and in the mature animal. J Lipid Res. 2004 Aug;45(8):1375-97. [Content Brief]
[3]. Fukui K, et al. Effect of Cholesterol Reduction on Receptor Signaling in Neurons. J Biol Chem. 2015 Sep 14. [Content Brief]
[4]. Puskás LG, et al. Cholesterol diet-induced hyperlipidemia influences gene expression pattern of rat hearts: a DNA microarray study. FEBS Lett. 2004 Mar 26;562(1-3):99-104. [Content Brief]
[5]. Onody A, et al. Hyperlipidemia induced by a cholesterol-rich diet leads to enhanced peroxynitrite formation in rat hearts. Cardiovasc Res. 2003 Jun 1;58(3):663-70. [Content Brief]
[6]. Baumer Y, et al. Hyperlipidemia-induced cholesterol crystal production by endothelial cells promotes atherogenesis. Nat Commun. 2017 Oct 24;8(1):1129. [Content Brief]
[7]. Finking G, et al. Nikolaj Nikolajewitsch Anitschkow (1885-1964) established the cholesterol-fed rabbit as a model for atherosclerosis research. Atherosclerosis. 1997 Nov;135(1):1-7. [Content Brief]
[8]. Fan J, et al. Use of Rabbit Models to Study Atherosclerosis. Methods Mol Biol. 2022;2419:413-431. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol | 1 mM | 2.5863 mL | 12.9316 mL | 25.8632 mL | 64.6580 mL |
| 5 mM | 0.5173 mL | 2.5863 mL | 5.1726 mL | 12.9316 mL | |
| 10 mM | 0.2586 mL | 1.2932 mL | 2.5863 mL | 6.4658 mL | |
| 15 mM | 0.1724 mL | 0.8621 mL | 1.7242 mL | 4.3105 mL | |
| 20 mM | 0.1293 mL | 0.6466 mL | 1.2932 mL | 3.2329 mL | |
| 25 mM | 0.1035 mL | 0.5173 mL | 1.0345 mL | 2.5863 mL |