Cholesterol (Water Soluble)

Cat. No.: HY-N0322A Purity: 98.0%: Data Sheet
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Cholesterol Water Soluble is a major sterol in mammals, constituting 20-25% of the structural composition of the plasma membrane. The plasma membrane is highly permeable to water but relatively impermeable to ions and protons. Cholesterol Water Soluble plays an important role in determining the fluidity and permeability characteristics of membranes and the function of transporters and signaling proteins. Cholesterol Water Soluble is also an endogenous estrogen-related receptor alpha (ERRα) agonist. Cholesterol Water Soluble can be used to study the effects of cholesterol on potassium currents in inner hair cells.
(Note: This product is a mixture of Cholesterol and Methyl-β-cyclodextrin. The product specifications below only indicate the effective content of Cholesterol.)

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample (0.1 - 0.2 mg)	Apply Now
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 85 publication(s) in Google Scholar

Other Forms of Cholesterol (Water Soluble):

Cholesterol (from animal) In-stock

85 Publications Citing Use of MCE Cholesterol (Water Soluble)

Bio/Physico-chemical Assay

Cell Imaging/Staining

Cell Proliferation/Viability Assay

: Cholesterol (Water Soluble) purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2025 May 26;24(1):151. [Abstract]; Analysis of interactions between GP73 and Cholesterol (0.1-10000 nM, 3 h), 25-HC, 27-HC, campesterol, and epicholesterol by MST.

: Cholesterol (Water Soluble) purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2025 May 26;24(1):151. [Abstract]; Analysis of interactions between Cholesterol (0.1-1000 nM, 3 h) and mouse GP73 (WT), the GP73 CRAC domain deletion mutant (∆CRAC) and the CARC Phe90/CRAC Tyr99 double mutant of mouse GP73 (F90I-Y99I, DM) by MST.

: Cholesterol (Water Soluble) purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2025 May 26;24(1):151. [Abstract]; Titration curves showing changes in the intrinsic fluorescence of WT, Y99I, F90I-Y99I (DM), or scrambled peptides (5 µM) in the presence of increasing concentrations of Cholesterol (0-140 μM).

: Cholesterol (Water Soluble) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jun 20:e04195. [Abstract]; Dil staining of GF and BLNP （the formulation (50:38.5:10:1.5) included ionizable lipids (D‐Lin‐MC3‐DMA), Cholesterol, DSPC, and DMG‐PEG2000‐NH2, which were homogeneously mixed in the ethanol phase (12 mM)）@GF microspheres (scale bar = 50 µm).

: Cholesterol (Water Soluble) purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jan 2;15(1):162. [Abstract]; Detection of the exogenous cholesterol activity in SARS2 infection. Indicated Caco2 cells were infected with SARS2-Spseudoviruses and treated with cholesterol (0-200 μg/mL) at indicated concentrations. After 48 hours, viral infection was determined by measuring the intracellular luciferase activity. RLU, relative light unit.

: Cholesterol (Water Soluble) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Sep;10(27):e2206878. [Abstract]; Effects of cholesterol repletion (20 μg/mL; 4 days) on colony formation potential in SCC-1cisR and SCC-23cisR cells with SQLE depletion in the presence of cisplatin (n = 4) were assessed.

: Cholesterol (Water Soluble) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Sep;10(27):e2206878. [Abstract]; SCC-1cisR and SCC-23cisR cells transfected with siCTRL or siSQLE were incubated in medium with or without 20 μg/mL Cholesterol for 24 h.

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Description	Cholesterol Water Soluble is a major sterol in mammals, constituting 20-25% of the structural composition of the plasma membrane. The plasma membrane is highly permeable to water but relatively impermeable to ions and protons. Cholesterol Water Soluble plays an important role in determining the fluidity and permeability characteristics of membranes and the function of transporters and signaling proteins. Cholesterol Water Soluble is also an endogenous estrogen-related receptor alpha (ERRα) agonist. Cholesterol Water Soluble can be used to study the effects of cholesterol on potassium currents in inner hair cells^[1]^[2]. (Note: This product is a mixture of Cholesterol and Methyl-β-cyclodextrin. The product specifications below only indicate the effective content of Cholesterol.)
In Vitro	In solution of 1mM water-soluble cholesterol, the amplitude of K currents in isolated IHCs is reversely changed^[2]. Note: Product is approximately 40 mg of Cholesterol per gram containing a balance of Methyl-B-cyclodextrin. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Cholesterol (Water Soluble) Related Antibodies
Appearance	Solid
Color	White to off-white
SMILES	[Cholesterol (Water Soluble)]
Structure Classification	Steroids
Initial Source	Endogenous metabolite
Shipping	Room temperature in continental US; may vary elsewhere.
Storage	4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility	In Vitro: H₂O : 50 mg/mL (Need ultrasonic) DMSO : ≥ 33.33 mg/mL "≥" means soluble, but saturation unknown. Molarity Calculator Dilution Calculator Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Mass = Concentration × Volume × Molecular Weight Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final) This equation is commonly abbreviated as: C₁V₁ = C₂V₂ Concentration _(start) C1 × Volume _(start) V1 = Concentration _(final) C2 × Volume _(final) V2 In Vivo: Select the appropriate dissolution method based on your experimental animal and administration route. For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents: To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day. The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution. Protocol 1 Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline Solubility: ≥ 2.5 mg/mL; Clear solution This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL. Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution. Protocol 2 Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline) Solubility: ≥ 2.5 mg/mL; Clear solution This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear. Protocol 3 Add each solvent one by one: 10% DMSO 90% Corn Oil Solubility: ≥ 2.5 mg/mL; Clear solution This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully. Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly. For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time. The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution. Protocol 1 Add each solvent one by one: PBS Solubility: 33.33 mg/mL; Clear solution; Need ultrasonic In Vivo Dissolution Calculator Please enter the basic information of animal experiments: Dosage mg/kg Animal weight (per animal) g Dosing volume (per animal) μL Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments. Calculation results: Working solution concentration: mg/mL This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details. The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE). Tel: 609-228-6898 E-mail: [email protected] Technical Support: [email protected]
Purity & Documentation	Purity: 98.0% Select Batch: Data Sheet (270 KB) SDS (251 KB) COA (243 KB) Handling Instructions (2659 KB)
References	[1]. Kimitsuki T. Cholesterol influences potassium currents in inner hair cells isolated from guinea pig cochlea. Auris Nasus Larynx. 2017;44(1):46-51. [Content Brief] [2]. Nguyen TV, et al. Contribution of membrane cholesterol to outer hair cell lateral wall stiffness. Otolaryngol Head Neck Surg. 1998;119(1):14-20. [Content Brief]