Cholesterol (synthetic)

Cat. No.: HY-N0322E Purity: 99.55%: Data Sheet
COA

SDS
Handling Instructions
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Cholesterol (synthetic) is a synthetic form of cholesterol. It is a lipid that sustains vital functions. Cholesterol (synthetic) can be used in research on cardiovascular diseases.

For research use only. We do not sell to patients.

Cholesterol (synthetic) Chemical Structure

CAS No. : 57-88-5

Size	Stock	Quantity
Free Sample (0.1 - 0.2 mg)	Apply Now
Free Sample (0.1 - 0.2 mg)	Apply Now
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Other Forms of Cholesterol (synthetic):

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cholesterol (synthetic) is a synthetic form of cholesterol. It is a lipid that sustains vital functions. Cholesterol (synthetic) can be used in research on cardiovascular diseases^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	20 μM Compound: b1	Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 30822712]

Molecular Weight

386.65

Formula

C₂₇H₄₆O

CAS No.

57-88-5

Appearance

Solid

Color

White to off-white

SMILES

O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@@H](CCCC(C)C)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

Ethanol : 7.14 mg/mL (18.47 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5863 mL	12.9316 mL	25.8632 mL
5 mM	0.5173 mL	2.5863 mL	5.1726 mL
10 mM	0.2586 mL	1.2932 mL	2.5863 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 0.71 mg/mL (1.84 mM); Clear solution

This protocol yields a clear solution of ≥ 0.71 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (7.1 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.55%

Select Batch:

Data Sheet (269 KB) SDS (394 KB)

COA (246 KB) HNMR (176 KB) RP-HPLC (169 KB) MS (119 KB)

Handling Instructions (2659 KB)

References

[1]. Chaudhuri NK, et al. A new synthesis of cholesterol and related C27 steroids. J Org Chem. 1969 Dec;34(12):3767-74.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol	1 mM	2.5863 mL	12.9316 mL	25.8632 mL	64.6580 mL
	5 mM	0.5173 mL	2.5863 mL	5.1726 mL	12.9316 mL
	10 mM	0.2586 mL	1.2932 mL	2.5863 mL	6.4658 mL
	15 mM	0.1724 mL	0.8621 mL	1.7242 mL	4.3105 mL