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BODIPY-Cholesterol 

Cat. No.: HY-125746
Handling Instructions

BODIPY-cholesterol is an intrinsically lipophilic, and cell-permeable analog of cholesterol with a fluorescent BODIPY group. BODIPY-cholesterol can be used to monitor sterol uptake and inter-organelle sterol flux in cells. (Excitation/Emission: 480/508 nm).

For research use only. We do not sell to patients.

BODIPY-Cholesterol Chemical Structure

BODIPY-Cholesterol Chemical Structure

CAS No. : 878557-19-8

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Description

BODIPY-cholesterol is an intrinsically lipophilic, and cell-permeable analog of cholesterol with a fluorescent BODIPY group. BODIPY-cholesterol can be used to monitor sterol uptake and inter-organelle sterol flux in cells. (Excitation/Emission: 480/508 nm)[1].

In Vitro

BODIPY-cholesterol assay
To prepare a stock solution of BODIPY-cholesterol for this assay, the analogue is reconstituted in DMSO to a stock concentration of 1-10 μM[1].
Cells diluted with PBS (or other buffers) are washed (300×g, 10 min) to remove excess diluent and the loose pellet resuspended with vehicle media to a proper concentration[1].
Cell suspensions are labeled with BODIPY-cholesterol (final concentration of 0.5-5 µM), mixed thoroughly and incubated for 10 -20 mins at 37 °C[1].
The cells are diluted with each of the capacitation media conditions as outlined in Incubation media to a final concentration and incubated for 2 h prior to flow cytometric assessment[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

576.61

Formula

C₃₆H₅₁BF₂N₂O

CAS No.
SMILES

C[[email protected]@]12[[email protected]](CC[[email protected]]2([H])[[email protected]](C)CCC(C3=C(C)C=C(C)[N-]3[B+3]([F-])4[F-])=C5[N]4=C(C)C=C5C)([H])[[email protected]@]6([H])[[email protected]@](CC1)([H])[[email protected]@]7(C(C[[email protected]](CC7)O)=CC6)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
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BODIPY-CholesterolOthersInhibitorinhibitorinhibit

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BODIPY-Cholesterol
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