1. Biochemical Assay Reagents
  2. Methyl-β-cyclodextrin

Methyl-β-cyclodextrin  (Synonyms: Methyl-beta-cyclodextrin)

Cat. No.: HY-101461 Assay: 99.95%
COA Handling Instructions

Methyl-β-cyclodextrin (Methyl-beta-cyclodextrin) is a cyclic heptasaccharide used to deliver hydrophobic drugs based on its property of solubilizing non-polar substances. Methyl-β-cyclodextrin is also extensively used as a cholesterol-depleting reagent. Methyl-β-cyclodextrin strongly reduces clathrin-dependent endocytosis. Methyl-β-cyclodextrin blocks cell migrasome formation.

For research use only. We do not sell to patients.

Methyl-β-cyclodextrin Chemical Structure

Methyl-β-cyclodextrin Chemical Structure

CAS No. : 128446-36-6

Size Price Stock Quantity
500 mg USD 55 In-stock
Estimated Time of Arrival: December 31
1 g USD 66 In-stock
Estimated Time of Arrival: December 31
5 g USD 92 In-stock
Estimated Time of Arrival: December 31
10 g USD 132 In-stock
Estimated Time of Arrival: December 31
25 g USD 251 In-stock
Estimated Time of Arrival: December 31
50 g USD 317 In-stock
Estimated Time of Arrival: December 31
> 100 g   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 30 publication(s) in Google Scholar

Top Publications Citing Use of Products

29 Publications Citing Use of MCE Methyl-β-cyclodextrin

IF

    Methyl-β-cyclodextrin purchased from MCE. Usage Cited in: J Med Virol. 2022 Nov 1.  [Abstract]

    Pretreatment with AY9944 (15, 30 µM), U18666A (5, 10 µM), lovastatin (5, 10 µM) and Methyl-β-cyclodextrin (MβCD; 5, 10 mM) for 2 h can all significantly reduce the level of EV-A71 VP1 protein in HCT-8 cell.
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Methyl-β-cyclodextrin (Methyl-beta-cyclodextrin) is a cyclic heptasaccharide used to deliver hydrophobic drugs based on its property of solubilizing non-polar substances. Methyl-β-cyclodextrin is also extensively used as a cholesterol-depleting reagent[1]. Methyl-β-cyclodextrin strongly reduces clathrin-dependent endocytosis[2]. Methyl-β-cyclodextrin blocks cell migrasome formation[3].

    In Vitro

    Methyl-β-cyclodextrin is extensively used to increase the permeability of cells, and thereby increase the uptake of small molecules such as glucose and nano-particles[4].
    Cyclodextrins are a family of cyclic oligosaccharides with a hydrophilic outer surface and a lipophilic central cavity. Cyclodextrins molecules are relatively large with a number of hydrogen donors and acceptors and, thus in general, they do not permeate lipophilic membranes. In the pharmaceutical industry, cyclodextrins have mainly been used as complexing agents to increase aqueous solubility of poorly soluble drugs and to increase their bioavailability and stability. Cyclodextrins are used in pharmaceutical applications for numerous purposes, including improving the bioavailability of drugs[4].
    Methyl-β-cyclodextrin quickly induces caspase-dependent apoptosis in PEL cells via cholesterol depletion from the plasma membrane. Methyl-β-cyclodextrin inhibits the growth of all PEL cell lines in a dose-dependent manner. The IC50 is 3.33-4.23 mM in each cell line[5].
    Methyl-β-cyclodextrin is a highly water soluble cyclic heptasaccharide consisting of a β-glucopyranose unit, has been reported as the most effective agent for the depletion of cholesterol from cells among the various cholesterol-depleting agents[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    In a PEL xenograft mouse model, Methyl-β-cyclodextrin significantly inhibits the growth and invasion of PEL cells without apparent adverse effects. Methyl-β-cyclodextrin-treated mice appears to be healthy, whereas non-treated mice has a distended abdominal region. The body weights of control are significantly higher than those of Methyl-β-cyclodextrin treated mice. Methyl-β-cyclodextrin-treated mice has a significantly lower volume of ascites than that of non-treated mice[4].
    Studies in both humans and animals have shown that cyclodextrins can be used to improve drug delivery from almost any type of drug formulation. Currently, there are approximately 30 different pharmaceutical products worldwide containing drug/cyclodextrins complexes in the market[6].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    1310 (Average)

    CAS No.
    SMILES

    CO[[email protected]@H]1[[email protected]](O[R])[[email protected]](O[R])[[email protected]@H](C)C(CO[R])O1.[7].[R=H or -CH3]

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL

    H2O : ≥ 50 mg/mL

    *"≥" means soluble, but saturation unknown.

    Purity & Documentation

    Purity: 99.95%

    References
    Cell Assay
    [1]

    PEL cells are incubated in triplicate in a 96-well microculture plate in the presence of different concentrations of methyl-β-cyclodextrin (0-10 mM) in a final volume of 0.1 mL for 24 h at 37°C. Subsequently, MTT (0.5 mg/mL final concentration) is added to each well. After 3 h of additional incubation, 100 μL of a 0.04 N HCl is added to dissolve the crystals. Absorption values at 570 nm are determined[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [1]

    Mice: Female NRJ mice are intraperitoneally inoculated with BCBL-1 cells suspended in PBS. The mice are then treated with intraperitoneal injections of PBS or methyl-β-cyclodextrin (500 mg/kg per day). Tumor burdens are evaluated by measuring body weights and ascites[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

     

    Salutation

    Applicant Name *

     

    Email address *

    Phone number *

     

    Organization name *

    Department *

     

    Requested quantity *

    Country or Region *

         

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Methyl-β-cyclodextrin
    Cat. No.:
    HY-101461
    Quantity:
    MCE Japan Authorized Agent: