DOTAP chloride (Synonyms: 1,2-Dioleoyl-3-trimethylammonium-propane chloride)

Name: DOTAP chloride
Brand: MedChemExpress
SKU: HY-112754A
Rating: 5.0 (14 reviews)

Cat. No.: HY-112754A Purity: 99.91%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

DOTAP chloride is a useful and effective cationic lipid for transient and stable transfection DNA (plasmids, bacmids) and modified nucleic acids (antisense oligonucleotides) with out the use of helper lipid.

For research use only. We do not sell to patients.

CAS No. : 132172-61-3

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid or liquid
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
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1 g	Get quote

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Customer Review

Based on 14 publication(s) in Google Scholar

Other Forms of DOTAP chloride:

14 Publications Citing Use of MCE DOTAP chloride

Gel Electrophoresis

: DOTAP chloride purchased from MedChemExpress. Usage Cited in: Pharmaceutics. 2023 Apr 30;15(5):1379.; (A) Identification of miR-126 conjugates using gel retardation assay. 1, miR-126; 2, Va-CLs; 3–8, DOTAP (DOTAP chloride):miR-126 = 16:1, 24:1, 32:1, 40:1, 48:1 and 56:1 incubated for 30 min at room temperature to obtain Va-CLs/miR-126 and CLs/miR-126 complexes. (B) The gray value of each miR-126 band was calculated

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

IC50: Cationic Lipid^[1]

In Vitro

liposomes are reorganized by associating with DNA. The transfection efficiency of DOTAP liposomes is mainly influenced by lipid composition and cell type, but not by size or zeta potential^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

698.54

Formula

C₄₂H₈₀ClNO₄

CAS No.

132172-61-3

Appearance

<35°C Solid,>38°C Liquid

Color

White to off-white

SMILES

CCCCCCCC/C=C\CCCCCCCC(OCC(OC(CCCCCCC/C=C\CCCCCCCC)=O)C[N+](C)(C)C)=O.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

Ethanol : 130 mg/mL (186.10 mM; Need ultrasonic)

DMSO : 25 mg/mL (35.79 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4316 mL	7.1578 mL	14.3156 mL
5 mM	0.2863 mL	1.4316 mL	2.8631 mL
10 mM	0.1432 mL	0.7158 mL	1.4316 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (3.58 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (266 KB) SDS (394 KB)

COA (242 KB) HNMR (291 KB) RP-HPLC (206 KB) MS (70 KB)

Handling Instructions (2659 KB)

References

[1]. Kim BK, et al. DOTAP/DOPE ratio and cell type determine transfection efficiency with DOTAP-liposomes.Biochim Biophys Acta. 2015 Oct;1848(10 Pt A):1996-2001. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / Ethanol	1 mM	1.4316 mL	7.1578 mL	14.3156 mL	35.7889 mL
	5 mM	0.2863 mL	1.4316 mL	2.8631 mL	7.1578 mL
	10 mM	0.1432 mL	0.7158 mL	1.4316 mL	3.5789 mL
	15 mM	0.0954 mL	0.4772 mL	0.9544 mL	2.3859 mL
	20 mM	0.0716 mL	0.3579 mL	0.7158 mL	1.7894 mL
	25 mM	0.0573 mL	0.2863 mL	0.5726 mL	1.4316 mL
	30 mM	0.0477 mL	0.2386 mL	0.4772 mL	1.1930 mL
Ethanol	40 mM	0.0358 mL	0.1789 mL	0.3579 mL	0.8947 mL
	50 mM	0.0286 mL	0.1432 mL	0.2863 mL	0.7158 mL
	60 mM	0.0239 mL	0.1193 mL	0.2386 mL	0.5965 mL
	80 mM	0.0179 mL	0.0895 mL	0.1789 mL	0.4474 mL
	100 mM	0.0143 mL	0.0716 mL	0.1432 mL	0.3579 mL

DOTAP chloride Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DOTAP132172-61-31,2-Dioleoyl-3-trimethylammonium-propaneLiposomeCationicLipidtransfectionnucleicacidInhibitorinhibitorinhibit

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DOTAP chloride (Synonyms: 1,2-Dioleoyl-3-trimethylammonium-propane chloride)

Customer Review

Other Forms of DOTAP chloride:

DOTAP chloride Related Antibodies

14 Publications Citing Use of MCE DOTAP chloride

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

DOTAP chloride Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

DOTAP chloride Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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