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DOTAP chloride (Synonyms: 1,2-Dioleoyl-3-trimethylammonium-propane chloride)

Cat. No.: HY-112754A Purity: >98.0%
Handling Instructions

DOTAP chloride is a useful and effective cationic lipid for transient and stable transfection DNA (plasmids, bacmids) and modified nucleic acids (antisense oligonucleotides) with out the use of helper lipid.

For research use only. We do not sell to patients.

DOTAP chloride Chemical Structure

DOTAP chloride Chemical Structure

CAS No. : 132172-61-3

Size Price Stock Quantity
10 mM * 1  mL in DMSO USD 61 In-stock
Estimated Time of Arrival: December 31
50 mg USD 55 In-stock
Estimated Time of Arrival: December 31
100 mg USD 100 In-stock
Estimated Time of Arrival: December 31
250 mg USD 220 Get quote
500 mg   Get quote  
1 g   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

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Description

DOTAP chloride is a useful and effective cationic lipid for transient and stable transfection DNA (plasmids, bacmids) and modified nucleic acids (antisense oligonucleotides) with out the use of helper lipid[1].

IC50 & Target

IC50: Cationic Lipid[1]

In Vitro

liposomes are reorganized by associating with DNA. The transfection efficiency of DOTAP liposomes is mainly influenced by lipid composition and cell type, but not by size or zeta potential[1].

Molecular Weight

698.54

Formula

C₄₂H₈₀ClNO₄

CAS No.

132172-61-3

SMILES

CCCCCCCC/C=C\CCCCCCCC(OCC(OC(CCCCCCC/C=C\CCCCCCCC)=O)C[N+](C)(C)C)=O.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under argon

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under argon)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (178.94 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4316 mL 7.1578 mL 14.3156 mL
5 mM 0.2863 mL 1.4316 mL 2.8631 mL
10 mM 0.1432 mL 0.7158 mL 1.4316 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (2.98 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (2.98 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: >98.0%

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This equation is commonly abbreviated as: C1V1 = C2V2

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Keywords:

DOTAP1,2-Dioleoyl-3-trimethylammonium-propaneOthersCationicLipidtransfectionnucleicacidInhibitorinhibitorinhibit

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Product Name:
DOTAP chloride
Cat. No.:
HY-112754A
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