2. Metabolic Enzyme/Protease
  3. Liposome
  4. DOTAP methylsulfate

DOTAP methylsulfate  (Synonyms: 1,2-Dioleoyl-3-trimethylammonium-propane methylsulfate)

Cat. No.: HY-W127378 Purity: 99.94%
COA
SDS
Handling Instructions Technical Support

DOTAP methylsulfat is a cationic lipid reagent, a cationic derivative of trimethylammonium, linked to two 18-carbon fatty acid tails, each with a single olefin group. DOTAP methylsulfat can self-assemble with negatively charged ions (such as DNA) to form complexes, which can be adsorbed to the cell membrane surface and enter the cell by electrostatic interaction and endocytosis, respectively. DOTAP methylsulfat promotes endosomal membrane fusion with its own hydrophobic domain, releases DNA into the cytoplasm, and exerts gene delivery function. DOTAP methylsulfat can be widely used in research fields such as gene therapy, cell transfection, and non-viral vector design.

For research use only. We do not sell to patients.

CAS No. : 144189-73-1

Size Price Stock Quantity
Solution
10 mM * 1 mL in Ethanol In-stock
Solid
25 mg In-stock
50 mg In-stock
100 mg In-stock
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Customer Review

Based on 14 publication(s) in Google Scholar

Other Forms of DOTAP methylsulfate:

DOTAP methylsulfate Related Antibodies

Top Publications Citing Use of Products

    DOTAP methylsulfate purchased from MedChemExpress. Usage Cited in: Pharmaceutics. 2023 Apr 30;15(5):1379.

    (A) Identification of miR-126 conjugates using gel retardation assay. 1, miR-126; 2, Va-CLs; 3–8, DOTAP (DOTAP chloride):miR-126 = 16:1, 24:1, 32:1, 40:1, 48:1 and 56:1 incubated for 30 min at room temperature to obtain Va-CLs/miR-126 and CLs/miR-126 complexes. (B) The gray value of each miR-126 band was calculated

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    DOTAP methylsulfat is a cationic lipid reagent, a cationic derivative of trimethylammonium, linked to two 18-carbon fatty acid tails, each with a single olefin group. DOTAP methylsulfat can self-assemble with negatively charged ions (such as DNA) to form complexes, which can be adsorbed to the cell membrane surface and enter the cell by electrostatic interaction and endocytosis, respectively. DOTAP methylsulfat promotes endosomal membrane fusion with its own hydrophobic domain, releases DNA into the cytoplasm, and exerts gene delivery function. DOTAP methylsulfat can be widely used in research fields such as gene therapy, cell transfection, and non-viral vector design[1][2].

    In Vitro

    Different mass ratios of DOTAP methylsulfat (different mass ratios; 4 hours transfection + 24 hours culture) and DOPE (HY-112005) have different optimal transfection efficiencies. In Huh7 and AGS cells, the optimal ratio is T1P0 (1:0)/T3P1 (3:1), and in A549 cells, the optimal ratio is T1P1 (1:1)/T3P1 (1:3), which is independent of liposome size or zeta potential[1].
    DOTAP methylsulfat mixed with lipids in certain ratios (e.g., 1:2, 1:1, 2:1) is more efficient than DOTAP methylsulfat alone in transfecting DNA, and still maintains transfection activity at 80% serum concentration. And the optimal transfection ratio of DOTAP methylsulfat to DNA is 12:1[2].

    DOTAP methylsulfat (30 min DNase treatment) DNA-lipid complexes remain stable to DNase I digestion in the presence of serum, and the addition of cholesterol does not affect the structural integrity of the complex[2].

    DOTAP methylsulfat (containing 1% NBD-DOTAP methylsulfat label; 2 h transfection) in flow cytometric analysis, cholesterol-modified liposomes bind to cells at a significantly higher rate in serum than pure DOTAP methylsulfat liposomes[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DOTAP methylsulfate Related Antibodies
    Molecular Weight

    774.19

    Formula

    C43H83NO8S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    S(OC)(=O)(=O)[O-].C(OC(CCCCCCC/C=C\CCCCCCCC)=O)(COC(CCCCCCC/C=C\CCCCCCCC)=O)C[N+](C)(C)C
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    Ethanol : ≥ 100 mg/mL (129.17 mM)

    DMSO : 5 mg/mL (6.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.2917 mL 6.4584 mL 12.9167 mL
    5 mM 0.2583 mL 1.2917 mL 2.5833 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% EtOH    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (3.23 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    • Protocol 2

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1.67 mg/mL (2.16 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.94%

    SDS (645 KB)

    COA (238 KB) HNMR (273 KB) RP-HPLC (236 KB) MS (71 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / Ethanol 1 mM 1.2917 mL 6.4584 mL 12.9167 mL 32.2918 mL
    5 mM 0.2583 mL 1.2917 mL 2.5833 mL 6.4584 mL
    Ethanol 10 mM 0.1292 mL 0.6458 mL 1.2917 mL 3.2292 mL
    15 mM 0.0861 mL 0.4306 mL 0.8611 mL 2.1528 mL
    20 mM 0.0646 mL 0.3229 mL 0.6458 mL 1.6146 mL
    25 mM 0.0517 mL 0.2583 mL 0.5167 mL 1.2917 mL
    30 mM 0.0431 mL 0.2153 mL 0.4306 mL 1.0764 mL
    40 mM 0.0323 mL 0.1615 mL 0.3229 mL 0.8073 mL
    50 mM 0.0258 mL 0.1292 mL 0.2583 mL 0.6458 mL
    60 mM 0.0215 mL 0.1076 mL 0.2153 mL 0.5382 mL
    80 mM 0.0161 mL 0.0807 mL 0.1615 mL 0.4036 mL
    100 mM 0.0129 mL 0.0646 mL 0.1292 mL 0.3229 mL
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    DOTAP methylsulfate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DOTAP144189-73-11,2-Dioleoyl-3-trimethylammonium-propaneLiposomebiochemical assayreagentInhibitorinhibitorinhibit

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