SM-102
Based on 50 publication(s) in Google Scholar
SM-102 is an amino cationic lipid useful in the formation of lipid nanoparticles (LNPs). SM-102 has higher transfection efficiency. SM-102 plays an important role in the effectiveness of lipid nanoparticles (LNPs) in delivering mRNA therapeutics and vaccines.
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- Pureté: 99.97%
- CAS No.: 2089251-47-6
- Formule: C44H87NO5
- Masse moléculaire:710.17
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Stockage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
Publications Citing Use of MedChemExpress (MCE) SM-102
More- Science. 2025 Apr 4;388(6742):eads4539. [Abstract]
- Nat Nanotechnol. 2026 Jan 14. [Abstract]
- Nat Nanotechnol. 2023 Sep;18(9):1105-1114. [Abstract]
- Adv Mater. 2024 Jun 19:e2404830. [Abstract]
- Nat Biomed Eng. 2024 Nov;8(11):1412-1424. [Abstract]
- Nat Biomed Eng. 2024 Dec;8(12):1715-1729. [Abstract]
- Nat Biomed Eng. 2024 Apr;8(4):361-379. [Abstract]
- Bioact Mater. 2024 May 23:39:273-286. [Abstract]
- Nat Chem. 2024 Oct;16(10):1687-1697. [Abstract]
- ACS Nano. 2024 Nov 26;18(47):32401-32420. [Abstract]
- ACS Nano. 2024 Oct 1;18(39):27077-27089. [Abstract]
- Nat Commun. 2024 Feb 26;15(1):1762. [Abstract]
- J Biomed Sci. 2023 Jun 28;30(1):46. [Abstract]
- Mol Ther. 2026 Jun 26:S1525-0016(26)00511-3. [Abstract]
- Redox Biol. 2026 May:92:104101. [Abstract]
- J Control Release. 2026 May 22.
- J Control Release. 2026 Mar 10:391:114607. [Abstract]
- J Control Release. 2025 May 3:113811. [Abstract]
- J Control Release. 2025 May 6:113821. [Abstract]
- J Control Release. 2024 Aug 2:373:727-737. [Abstract]
- Small Struct. 2026 Mar 6;7(3):e202500583.
- J Colloid Interface Sci. 2024 Jun 23:674:139-144. [Abstract]
- Adv Healthc Mater. 2024 Jul 23:e2400366. [Abstract]
- ACS Appl Mater Interfaces. 2024 Dec 11;16(49):67192-67202. [Abstract]
- Cell Rep. 2025 Aug 22;44(9):116170. [Abstract]
- NPJ Vaccines. 2025 Dec 19. [Abstract]
- Int J Nanomedicine. 2025 Oct 22:20:12783-12804. [Abstract]
- Int J Nanomedicine. 2023 Dec 19:18:7785-7801. [Abstract]
- Mol Ther Nucleic Acids. 2026 Mar 5;37(2):102886. [Abstract]
- Pharmaceutics. 2026 Feb 11;18(2):228. [Abstract]
- Eur J Pharm Sci. 2026 Jul 1:222:107556. [Abstract]
- Eur J Pharm Sci. 2026 Jan 1:216:107383. [Abstract]
- Mol Pharm. 2024 Nov 4;21(11):5672-5680. [Abstract]
- Cell Rep Methods. 2024 Jan 22;4(1):100673. [Abstract]
- Eur J Pharm Biopharm. 2026 Jun 9:115146. [Abstract]
- Hum Gene Ther. 2025 Jun;36(11-12):870-883. [Abstract]
- J Chromatogr A. 2025 Apr 12:1746:465788. [Abstract]
- Anal Bioanal Chem. 2024 Oct;416(24):5281-5293. [Abstract]
- Vaccines. 2024 Jun 26;12(7):714. [Abstract]
- Electrophoresis. 2025 Sep 28. [Abstract]
- SSRN. 2026 Jun 18.
- SSRN. 2025 Oct 31.
- SSRN. 2025 Oct 29.
- SSRN. 2025 Oct 2.
- bioRxiv. 2025 Sep 29.
- SSRN. 2025 May 6.
- bioRxiv. 2025 Mar 1:2025.02.25.640222. [Abstract]
- bioRxiv. 2025 January 21.
- bioRxiv. 2024 Feb 5:2024.02.03.578630. [Abstract]
- Research Square Preprint. 2023 Aug 3.
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In Vivo Imaging
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Cell Proliferation/Viability Assay
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Others
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Others
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Flow Cytometry
Activité biologique
Note: This product is a viscous liquid. To minimize product loss, it is highly recommended to centrifuge the bottle and dissolve the entire contents before aliquoting for storage; please avoid attempting to weigh out the material yourself.
Preparation of Lipid Nanoparticles[3][4]
Here we provide lipid molar ratios for LNPs in FDA-approved mRNA-1273 (a COVID-19 mRNA vaccine). The molar ratio of lipids in this formulation is SM-102 : DSPC : Cholesterol : DMG-PEG 2000 = 50 : 10 : 38.5 : 1.5[1], and RNA to lipid weight ratio is 0.05 (wt/wt).
A. Lipid Mixture Preparation
1. Dissolve lipids in ethanol and prepare 10 mg/mL stock solutions. The lipid stock solutions can be stored at 20°C for later use.
Note 1: The ionizable lipid is usually a liquid. Due to the viscosity, it should always be weighed rather than relying on the autopipette volume.
Note 2: Cholesterol in solution should be kept warm (>37°C) to maintain fluidity. Transfer the cholesterol solution promptly to avoid cooling.
2. Prepare the lipid mixture solution as described. For each mL of lipid mixture add the following: 572 μL of 10 mg/mL SM-102 (HY-134541), 240 μL of 10 mg/mL Cholesterol (HY-N0322), 127 μL of 10 mg/mL DSPC (HY-W040193), and 61 μL of DMG-PEG 2000 (HY-112764). Mix the solutions thoroμghly to achieve a clear solution. This mixture contains 10 mg of total lipid.
Note 3: The choice of lipids and ratios may be changed as desired and this will affect the LNP properties (size, polydispersity, and efficacy) and the amount of mRNA required.
B. mRNA Preparation
1. Prepare a 166.7 μg/mL mRNA solution with 100 mM pH 5 sodium acetate buffer.
Note 4: The lipid:mRNA weight ratio influences the encapsulation efficiency. Other weight ratios may be prepared as alternative formulations and should be adjusted accordingly by user.
C. Mixing
There are three commonly used methods to achieve rapid mixing of the solutions: the pipette mixing method, the vortex mixing method, and the microfluidic mixing method. All these mixing methods can be used for various applications.
It is important to note that pipette mixing method and vortex mixing method may yield more heterogeneous LNPs with lower encapsulation efficiencies and is prone to variability. Microfluidic devices enable rapid mixing in a highly controllable, reproducible manner that achieves homogeneous LNPs and high encapsulation efficiency. Within these devices, the ethanolic lipid mixture and aqueous solution are rapidly combined in individual streams. LNPs are formed as the two streams mix and are then collected into a single collection tube.
1. Pipette Mixing Method:
1.1. Pipette 3 mL of the mRNA solution and quickly add it into 1 mL of the lipid mixture solution (A 1:3 ratio of ethanolic lipid mixture to aqueous buffer is generally used.) Pipette up and down rapidly for 20-30 seconds.
1.2. Incubate the resulting solution at room temperature for up to 15 minutes.
1.3. After mixing, the LNPs were dialyzed against PBS (pH 7.4) for 2 h, sterile filtered using 0.2 μm filters, and stored at 4°C.
2. Vortex Mixing Method:
1.1. Vortex 3 mL of mRNA solution at a moderate speed on the vortex mixer. Then, Quickly add 1 mL of the lipid mixture solution into the vortexing solution (A 1:3 ratio of ethanolic lipid mixture to aqueous buffer is generally used.). Continue vortexing the resulting dispersion for another 20-30 seconds.
1.2. Incubate the resulting solution at room temperature for up to 15 minutes.
1.3. After mixing, the LNPs were dialyzed against PBS (pH 7.4) for 2 h, sterile filtered using 0.2 μm filters, and stored at 4°C.
3. Microfluidic Mixing Method:
1.1 The 3 mL of mRNA buffer solution and 1 mL of the lipid mixture solution were mixed at a total flow rate of 12 mL/min in a microfluidic device (A 1:3 ratio of ethanolic lipid mixture to aqueous buffer is generally used.).
Note 5: Parameters such as the flow rate ratio and total flow rate can be altered to fine-tune LNPs.
1.2. After mixing, the LNPs were dialyzed against PBS (pH 7.4) for 2 h, sterile filtered using 0.2 μm filters, and stored at 4°C.
Reference
1. Curr Issues Mol Biol. 2022 Oct 19;44(10):5013-5027.
2. Curr Protoc. 2023;3(9):e898.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2089251-47-6
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Appearance Liquid (Density: 0.925±0.06 g/cm3)
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Masse moléculaire 710.17
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Formule C44H87NO5
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Color Colorless to light yellow
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SMILES
O=C(OC(CCCCCCCC)CCCCCCCC)CCCCCCCN(CCO)CCCCCC(OCCCCCCCCCCC)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (50)
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Journal Impact Factor
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Most Recent
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Science
2025 Apr 4;388(6742):eads4539. PMID: 40179174 -
Nat Nanotechnol
A disease-severity-responsive nanoparticle enables potent ghrelin messenger RNA therapy in osteoarthritis. [Abstract]2026 Jan 14. PMID: 41535382 -
Nat Nanotechnol
Adjuvant lipidoid-substituted lipid nanoparticles augment the immunogenicity of SARS-CoV-2 mRNA vaccines. [Abstract]2023 Sep;18(9):1105-1114. PMID: 37365276 -
Adv Mater
pH-Responsive β-Glucans-Complexed mRNA in LNPs as an Oral Vaccine for Enhancing Cancer Immunotherapy. [Abstract]2024 Jun 19:e2404830. PMID: 38895941 -
Nat Biomed Eng
Optimization of the activity and biodegradability of ionizable lipids for mRNA delivery via directed chemical evolution. [Abstract]2024 Nov;8(11):1412-1424. PMID: 39578640 -
Nat Biomed Eng
2024 Dec;8(12):1715-1729. PMID: 39638875 -
Nat Biomed Eng
2024 Apr;8(4):361-379. PMID: 38486104 -
Bioact Mater
β-catenin mRNA encapsulated in SM-102 lipid nanoparticles enhances bone formation in a murine tibia fracture repair model. [Abstract]2024 May 23:39:273-286. PMID: 38832305 -
Nat Chem
Fast and facile synthesis of amidine-incorporated degradable lipids for versatile mRNA delivery in vivo. [Abstract]2024 Oct;16(10):1687-1697. PMID: 38982196
SM-102 purchased from MedChemExpress. Usage Cited in: Nat Chem. 2024 Oct;16(10):1687-1697. [Abstract]
Cytotoxicity of LNPs (SM-102 (or MC3) LNP was formulated by SM-102, DSPC, cholesterol and DMG-PEG at a molar ratio of 50:10:38.5:1.5 using microfluidic mixing). HepG2 cells were treated with the indicated mRNA dose for 24 h. The dashed line indicates 80% cell viability.
SM-102 purchased from MedChemExpress. Usage Cited in: Nat Chem. 2024 Oct;16(10):1687-1697. [Abstract]
Hemolysis test to assess membrane destructive activity(SM-102 (or MC3) LNP was formulated by SM-102, DSPC, cholesterol and DMG-PEG at a molar ratio of 50:10:38.5:1.5 using microfluidic mixing).
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ACS Nano
In Situ Tumor-Infiltrating Lymphocyte Therapy by Local Delivery of an mRNA Encoding Membrane-Anchored Anti-CD3 Single-Chain Variable Fragment. [Abstract]2024 Nov 26;18(47):32401-32420. PMID: 39527145 -
ACS Nano
2024 Oct 1;18(39):27077-27089. PMID: 39298422 -
Nat Commun
In situ combinatorial synthesis of degradable branched lipidoids for systemic delivery of mRNA therapeutics and gene editors. [Abstract]2024 Feb 26;15(1):1762. PMID: 38409275 -
J Biomed Sci
2023 Jun 28;30(1):46. PMID: 37380988 -
Mol Ther
Targeting vaccine fusion proteins to APCs increases immunogenicity of adenoviral and mRNA-LNP vaccines. [Abstract]2026 Jun 26:S1525-0016(26)00511-3. PMID: 42363600 -
Redox Biol
Stromal cell-derived itaconate promotes endometriosis via macrophage NRF2 and lysosomal pH modulation. [Abstract]2026 May:92:104101. PMID: 41780193 -
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J Control Release
Optimized lipid nanoparticles for pulmonary delivery of CRISPR/Cas9 targeting KRAS G12S in lung cancer. [Abstract]2026 Mar 10:391:114607. PMID: 41506374 -
J Control Release
Delivery of Itgb1-siRNA by triptolide-modified and anti-Flt1 peptide-guided ionizable cationic LNPs for targeted therapy of corneal neovascularization. [Abstract]2025 May 3:113811. PMID: 40324532 -
J Control Release
Impact of pre-existing anti-polyethylene glycol (PEG) IgM on biodistribution and humoral response of intramuscularly administered PEGylated mRNA loaded lipid nanoparticle. [Abstract]2025 May 6:113821. PMID: 40339658 -
J Control Release
Physicochemical and structural insights into lyophilized mRNA-LNP from lyoprotectant and buffer screenings. [Abstract]2024 Aug 2:373:727-737. PMID: 39059500 -
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J Colloid Interface Sci
2024 Jun 23:674:139-144. PMID: 38925059 -
Adv Healthc Mater
Redirecting Tumor Evolution with Nanocompiler Precision for Enhanced Therapeutic Outcomes. [Abstract]2024 Jul 23:e2400366. PMID: 39039965 -
ACS Appl Mater Interfaces
Gastrointestinal Device-Mediated Delivery of mRNA-Lipid Nanoparticles Achieves Distinct Expression and Biodistribution in Mice and Pigs. [Abstract]2024 Dec 11;16(49):67192-67202. PMID: 39621822 -
Cell Rep
2025 Aug 22;44(9):116170. PMID: 40849906 -
NPJ Vaccines
mRNA-lipid nanoparticle vaccines provide protection against lethal Nipah virus infection. [Abstract]2025 Dec 19. PMID: 41419782 -
Int J Nanomedicine
Optimized Lipid Nanoparticle-Mediated mRNA Co-Delivery of SOX5/SOX9 Enables Synergistic Senescence Reversal for Osteoarthritis Therapy. [Abstract]2025 Oct 22:20:12783-12804. PMID: 41146650
SM-102 purchased from MedChemExpress. Usage Cited in: Int J Nanomedicine. 2025 Oct 22:20:12783-12804. [Abstract]
Primary chondrocytes were treated with seven different LNP formulations loaded with luciferase mRNA (SM-102 molar ratios ranging from 40% to 60%), and the luminescence intensity was measured 12 hours post‑transfection.
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Int J Nanomedicine
Liver-Specific Ionizable Lipid Nanoparticles Mediated Efficient RNA Interference to Clear "Bad Cholesterol". [Abstract]2023 Dec 19:18:7785-7801. PMID: 38144512 -
Mol Ther Nucleic Acids
A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines. [Abstract]2026 Mar 5;37(2):102886. PMID: 41883582 -
Pharmaceutics
Unconventional Lysine-Type Lipid Assemblies Enable Efficient Antisense Oligonucleotide Delivery with Distinct Structural Features. [Abstract]2026 Feb 11;18(2):228. PMID: 41754970 -
Eur J Pharm Sci
Liposomes and lipid nanoparticles: a tutorial for advanced chemical and structural characterisation. [Abstract]2026 Jul 1:222:107556. PMID: 42142813 -
Eur J Pharm Sci
Impact of formulation and process parameters on the stability and bioactivity of RNA-loaded lipid nanoparticles during nebulization. [Abstract]2026 Jan 1:216:107383. PMID: 41265762 -
Mol Pharm
2024 Nov 4;21(11):5672-5680. PMID: 39324825 -
Cell Rep Methods
A fluorescent splice-switching mouse model enables high-throughput, sensitive quantification of antisense oligonucleotide delivery and activity. [Abstract]2024 Jan 22;4(1):100673. PMID: 38171361 -
Eur J Pharm Biopharm
Interactions between mRNA lipid nanoparticles and immune cells in fresh human whole blood. [Abstract]2026 Jun 9:115146. PMID: 42264006 -
Hum Gene Ther
Halting Recombinant Adeno-Associated Virus Transgene Expression Using mRNA-Lipid Nanoparticle-Delivered Meganucleases. [Abstract]2025 Jun;36(11-12):870-883. PMID: 40356311 -
J Chromatogr A
A platform method for simultaneous quantification of lipid and nucleic acid components in lipid nanoparticles. [Abstract]2025 Apr 12:1746:465788. PMID: 39987694 -
Anal Bioanal Chem
Development of an advanced separation and characterization platform for mRNA and lipid nanoparticles using multi-detector asymmetrical flow field-flow fractionation. [Abstract]2024 Oct;416(24):5281-5293. PMID: 39102094 -
Vaccines
Multivalent mRNA Vaccine Elicits Broad Protection against SARS-CoV-2 Variants of Concern. [Abstract]2024 Jun 26;12(7):714. PMID: 39066352
SM-102 purchased from MedChemExpress. Usage Cited in: Vaccines. 2024 Jun 26;12(7):714. [Abstract]
Summary table shows the molar ratio and physiochemical properties of the mRNA-LNPs (SM-102, DSPC, cholesterol, and DMG-PEG 2000 were mixed in a molar ratio of 50:10:38.5:1.5).
SM-102 purchased from MedChemExpress. Usage Cited in: Vaccines. 2024 Jun 26;12(7):714. [Abstract]
Flow cytometry data showed that each mRNA was successfully transfected and expressed 24 hours later (SM-102, DSPC, cholesterol, and DMG-PEG 2000 were mixed in a molar ratio of 50:10:38.5:1.5).
SM-102 purchased from MedChemExpress. Usage Cited in: Vaccines. 2024 Jun 26;12(7):714. [Abstract]
The binding activity of serum to different strains was detected by ELISA (SM-102, DSPC, cholesterol, and DMG-PEG 2000 were mixed in a molar ratio of 50:10:38.5:1.5).
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Electrophoresis
2025 Sep 28. PMID: 41015920 -
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bioRxiv
Plug-and-play assembly of biodegradable ionizable lipids for potent mRNA delivery and gene editing in vivo. [Abstract]2025 Mar 1:2025.02.25.640222. PMID: 40060499 -
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bioRxiv
2024 Feb 5:2024.02.03.578630. PMID: 38370774 -
Solvant et solubilité
Ethanol : ≥ 100 mg/mL (140.81 mM)
DMSO : 100 mg/mL (140.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (3.52 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (3.52 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Escalona-Rayo O, et al. In vitro and in vivo evaluation of clinically-approved ionizable cationic lipids shows divergent results between mRNA transfection and vaccine efficacy. Biomed Pharmacother. 2023 Sep;165:115065. [Content Brief]
[2]. Cho HY, et al. Effective Perturbations on the Amplitude and Hysteresis of Erg-Mediated Potassium Current Caused by 1-Octylnonyl 8-[(2-hydroxyethyl)[6-oxo-6(undecyloxy)hexyl]amino]-octanoate (SM-102), a Cationic Lipid. Biomedicines. 2021 Oct 1;9(10):1367. [Content Brief]
[3]. Mashima R, et al. Lipid Nanoparticles: A Novel Gene Delivery Technique for Clinical Application. Curr Issues Mol Biol. 2022 Oct 19;44(10):5013-5027. [Content Brief]
[4]. McKenzie RE, et al. mRNA Synthesis and Encapsulation in Ionizable Lipid Nanoparticles. Curr Protoc. 2023 Sep;3(9):e898. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol / DMSO | 1 mM | 1.4081 mL | 7.0406 mL | 14.0811 mL | 35.2028 mL |
| 5 mM | 0.2816 mL | 1.4081 mL | 2.8162 mL | 7.0406 mL | |
| 10 mM | 0.1408 mL | 0.7041 mL | 1.4081 mL | 3.5203 mL | |
| 15 mM | 0.0939 mL | 0.4694 mL | 0.9387 mL | 2.3469 mL | |
| 20 mM | 0.0704 mL | 0.3520 mL | 0.7041 mL | 1.7601 mL | |
| 25 mM | 0.0563 mL | 0.2816 mL | 0.5632 mL | 1.4081 mL | |
| 30 mM | 0.0469 mL | 0.2347 mL | 0.4694 mL | 1.1734 mL | |
| 40 mM | 0.0352 mL | 0.1760 mL | 0.3520 mL | 0.8801 mL | |
| 50 mM | 0.0282 mL | 0.1408 mL | 0.2816 mL | 0.7041 mL | |
| 60 mM | 0.0235 mL | 0.1173 mL | 0.2347 mL | 0.5867 mL | |
| 80 mM | 0.0176 mL | 0.0880 mL | 0.1760 mL | 0.4400 mL | |
| 100 mM | 0.0141 mL | 0.0704 mL | 0.1408 mL | 0.3520 mL |