INDO 1 pentapotassium
Based on 1 Customer Validation
INDO 1 pentapotassium is a cell-impermeable, dual-emission ratiometric fluorescent calcium indicator with a KD·β value of 0.44 μM. INDO 1 pentapotassium binds to intracellular free calcium ions and undergoes wavelength-dependent fluorescence changes. In the unbound state (free form) without Ca2+, its fluorescence emission peak is at 485 nm upon excitation with ultraviolet light (350 nm). When INDO 1 pentasodium binds to Ca2+ (bound state), the emission peak shifts to 405 nm (Ex = 350 nm; dual emission at Em= 405/485 nm).
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 96.43%
- CAS 番号: 132319-56-3
- 分子式: C32H26K5N3O12
- 分子量:840.05
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保管条件:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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生物活性
製品説明
体外実験
INDO 1 pentapotassium enables accurate measurement of absolute cytoplasmic Ca2+ levels in R1-R6 photoreceptors of white-eyed Oregon wild-type Drosophila melanogaster[1].
INDO 1 pentapotassium binds extensively to proteins isolated from rabbit ventricular myocytes[2].
INDO 1 pentapotassium is a calcium-sensitive fluorescent dye used in glass capillaries, with an apparent KD·β value of 0.44 μM; as calcium concentration increases, its emission at 405 nm enhances while emission at 470 nm diminishes[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
475
Excitation (Ex)
350
化学情報
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CAS 番号 132319-56-3
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性状 Solid
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分子量 840.05
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分子式 C32H26K5N3O12
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Color Off-white to light yellow
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SMILES
O=C(C1=CC2=C(C=C1)C=C(C3=CC=C(N(CC(O[K])=O)CC(O[K])=O)C(OCCOC4=CC(C)=CC=C4N(CC(O[K])=O)CC(O[K])=O)=C3)N2)O[K]
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶剤 & 溶解度
体外:
H2O : 62.5 mg/mL (74.40 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
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- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.1904 mL | 5.9520 mL | 11.9041 mL | 29.7601 mL |
| 5 mM | 0.2381 mL | 1.1904 mL | 2.3808 mL | 5.9520 mL | |
| 10 mM | 0.1190 mL | 0.5952 mL | 1.1904 mL | 2.9760 mL | |
| 15 mM | 0.0794 mL | 0.3968 mL | 0.7936 mL | 1.9840 mL | |
| 20 mM | 0.0595 mL | 0.2976 mL | 0.5952 mL | 1.4880 mL | |
| 25 mM | 0.0476 mL | 0.2381 mL | 0.4762 mL | 1.1904 mL | |
| 30 mM | 0.0397 mL | 0.1984 mL | 0.3968 mL | 0.9920 mL | |
| 40 mM | 0.0298 mL | 0.1488 mL | 0.2976 mL | 0.7440 mL | |
| 50 mM | 0.0238 mL | 0.1190 mL | 0.2381 mL | 0.5952 mL | |
| 60 mM | 0.0198 mL | 0.0992 mL | 0.1984 mL | 0.4960 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
INDO 1 pentapotassium 関連分類
HY-D0121A 関連分類
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