INDO 1
INDO 1 is a cell-impermeable, dual-emission ratiometric fluorescent calcium indicator with a KD·β value of 0.44 μM. INDO 1 binds to intracellular free calcium ions and undergoes wavelength-dependent fluorescence changes. In the unbound state (free form) without Ca2+, its fluorescence emission peak is at 485 nm upon excitation with ultraviolet light (350 nm). When INDO 1 pentasodium binds to Ca2+ (bound state), the emission peak shifts to 405 nm (Ex = 350 nm; dual emission at Em= 405/485 nm).
For research use only. We do not sell to patients.
- CAS No.: 96314-96-4
- Formula: C32H31N3O12
- Molecular Weight:649.60
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
INDO 1 enables accurate measurement of absolute cytoplasmic Ca2+ levels in R1-R6 photoreceptors of white-eyed Oregon wild-type Drosophila melanogaster[1].
INDO 1 binds extensively to proteins isolated from rabbit ventricular myocytes[2].
INDO 1 is a calcium-sensitive fluorescent dye used in glass capillaries, with an apparent KD·β value of 0.44 μM; as calcium concentration increases, its emission at 405 nm enhances while emission at 470 nm diminishes[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Emission (Em)
475
Excitation (Ex)
350
Chemical Information
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CAS No. 96314-96-4
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Molecular Weight 649.60
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Formula C32H31N3O12
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SMILES
O=C(C1=CC2=C(C=C1)C=C(C3=CC=C(N(CC(O)=O)CC(O)=O)C(OCCOC4=CC(C)=CC=C4N(CC(O)=O)CC(O)=O)=C3)N2)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Ca2+ Staining Technique
Ca2+ staining is an experimental technique that utilizes specific fluorescent probes (such as Fluo-4 AM, Fura-2, etc.) to qualitatively or quantitatively detect dynamic changes in intracellular Ca2+ concentrations; this is achieved by monitoring the changes in fluorescent signals generated when these probes bind to free intracellular calcium ions. The underlying principle relies primarily on the presence of chelating groups within the probe's molecular structure that possess high affinity for calcium ions.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
INDO 1 Related Classifications
HY-D0121 Related Classifications
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