Sulfane sulfur probe 4
Based on 1 Customer Validation
Sulfane sulfur probe 4 (SSP4) is a fluorescent indicator with high selectivity and sensitivity toward sulfane sulfur (including small-molecule sulfane sulfur and protein-bound sulfane sulfur), and its fluorescence is inhibited by Cu2+. Sulfane sulfur probe 4 produces a fluorescence turn-on response upon reaction with sulfane sulfur. Sulfane sulfur probe 4 can detect persulfidated proteins including papain, BSA, GAPDH, and human protein disulfide isomerase. Sulfane sulfur probe 4 enables bioimaging of exogenous and endogenous sulfane sulfur in cells.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté: 95.16%
- CAS No.: 1810731-98-6
- Formule: C34H20O7S2
- Masse moléculaire:604.65
-
Stockage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Activité biologique
-
Chemical Information
-
Solvant et solubilité
- Pureté et documentation
- Références
-
Help & FAQs
-
HY-D0074
Prodan -
HY-138226
BODIPY 558/568 C12 -
HY-126823
Phen Green SK diacetate (5/6-mixture) -
HY-DY1061
BODIPY 558/568 C12 (solution) -
HY-116425
N-1-Naphthylphthalamic acid -
HY-D1176
Calcium Green 1AM -
HY-D0121A
INDO 1 pentapotassium -
HY-W011209
N6-Isopentenyladenosine -
HY-N8440
Gibberellin A4 -
HY-N7443
Gibberellin A1 -
HY-135269
Quin II potassium -
HY-145612
Sudan red 7B
Activité biologique
Description
In Vitro
SSP4 (5 μM; 20 min) is a highly sensitive and highly selective fluorescent probe targeting small-molecule sulfane sulfur in cell-free systems, with a limit of detection of 28 nM. It exhibits optimal performance under pH 6.5-8 conditions and is hardly interfered with by most biomolecules except for high concentrations of GSH and Cu2+[1].
SSP4 (5-20 μM; 20-30 min) enables sensitive detection of exogenous and endogenous sulfane sulfur in HeLa, COS-7 and HEK293T cells. No cytotoxicity is observed at the relevant concentrations, and it can distinguish differences in intracellular sulfane sulfur levels based on genetic modification[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 1810731-98-6
-
Appearance Solid
-
Masse moléculaire 604.65
-
Formule C34H20O7S2
-
Color White to off-white
-
SMILES
O=C(C1=C(S)C=CC=C1)OC2=CC=C3C(OC4=CC(OC(C5=C(S)C=CC=C5)=O)=CC=C4C36OC(C7=C6C=CC=C7)=O)=C2
-
Synonyms
SSP4
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvant et solubilité
In Vitro:
DMSO : 83.33 mg/mL (137.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
-
Fiche technique (284 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6538 mL | 8.2692 mL | 16.5385 mL | 41.3462 mL |
| 5 mM | 0.3308 mL | 1.6538 mL | 3.3077 mL | 8.2692 mL | |
| 10 mM | 0.1654 mL | 0.8269 mL | 1.6538 mL | 4.1346 mL | |
| 15 mM | 0.1103 mL | 0.5513 mL | 1.1026 mL | 2.7564 mL | |
| 20 mM | 0.0827 mL | 0.4135 mL | 0.8269 mL | 2.0673 mL | |
| 25 mM | 0.0662 mL | 0.3308 mL | 0.6615 mL | 1.6538 mL | |
| 30 mM | 0.0551 mL | 0.2756 mL | 0.5513 mL | 1.3782 mL | |
| 40 mM | 0.0413 mL | 0.2067 mL | 0.4135 mL | 1.0337 mL | |
| 50 mM | 0.0331 mL | 0.1654 mL | 0.3308 mL | 0.8269 mL | |
| 60 mM | 0.0276 mL | 0.1378 mL | 0.2756 mL | 0.6891 mL | |
| 80 mM | 0.0207 mL | 0.1034 mL | 0.2067 mL | 0.5168 mL | |
| 100 mM | 0.0165 mL | 0.0827 mL | 0.1654 mL | 0.4135 mL |
Sulfane sulfur probe 4 Related Classifications
HY-158779 Related Classifications
Powered by Bioz