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Fluorescein (Uranine) is widely used as a fluorescent tracer in medicinal and biological applications and tumor infected tissues tracer. Fluorescein (Uranine) is a representative green fluorophore that has been widely used as a scaffold of practically useful green fluorescent probes.

For research use only. We do not sell to patients.

Fluorescein

Fluorescein Chemical Structure

CAS No. : 2321-07-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
250 mg In-stock
1 g In-stock
5 g In-stock
10 g In-stock
50 g   Get quote  

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Customer Review

Based on 13 publication(s) in Google Scholar

Other Forms of Fluorescein:

Fluorescein Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Fluorescein (Uranine) is widely used as a fluorescent tracer in medicinal and biological applications and tumor infected tissues tracer. Fluorescein (Uranine) is a representative green fluorophore that has been widely used as a scaffold of practically useful green fluorescent probes[1][2].

In Vitro

Fluorescein is a synthetic organic photoactive dye compound soluble in water, alcohol and polar solvents[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Fluorescein Related Antibodies
Molecular Weight

332.31

Formula

C20H12O5
CAS No.
Appearance

Solid

Color

Pink to red

Emission (Em)

525
Excitation (Ex)

488
SMILES

O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=CC=C5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

RT, protect from light, stored under nitrogen

In solvent -80°C 1 year
-20°C 6 months
Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (250.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0092 mL 15.0462 mL 30.0924 mL
5 mM 0.6018 mL 3.0092 mL 6.0185 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 96.32%

SDS (393 KB)

COA (254 KB) LCMS (87 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0092 mL 15.0462 mL 30.0924 mL 75.2310 mL
5 mM 0.6018 mL 3.0092 mL 6.0185 mL 15.0462 mL
10 mM 0.3009 mL 1.5046 mL 3.0092 mL 7.5231 mL
15 mM 0.2006 mL 1.0031 mL 2.0062 mL 5.0154 mL
20 mM 0.1505 mL 0.7523 mL 1.5046 mL 3.7615 mL
25 mM 0.1204 mL 0.6018 mL 1.2037 mL 3.0092 mL
30 mM 0.1003 mL 0.5015 mL 1.0031 mL 2.5077 mL
40 mM 0.0752 mL 0.3762 mL 0.7523 mL 1.8808 mL
50 mM 0.0602 mL 0.3009 mL 0.6018 mL 1.5046 mL
60 mM 0.0502 mL 0.2508 mL 0.5015 mL 1.2538 mL
80 mM 0.0376 mL 0.1881 mL 0.3762 mL 0.9404 mL
100 mM 0.0301 mL 0.1505 mL 0.3009 mL 0.7523 mL
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Fluorescein Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fluorescein2321-07-5UranineFluorescent DyeInhibitorinhibitorinhibit

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