1. Dye Reagents
  2. 5-FAM SE

5-FAM SE 

Cat. No.: HY-15938 Purity: ≥98.0%
Handling Instructions

5-FAM SE is a single isomer, is a fluorescent labeling reagent used for labeling peptides, proteins and nucleotides. 5-FAM SE can react with amines and can yield stable amine conjugates.

For research use only. We do not sell to patients.

5-FAM SE Chemical Structure

5-FAM SE Chemical Structure

CAS No. : 92557-80-7

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 184 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 184 In-stock
Estimated Time of Arrival: December 31
Solid
10 mg USD 167 In-stock
Estimated Time of Arrival: December 31
50 mg USD 552 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1008 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Other Forms of 5-FAM SE:

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Description

5-FAM SE is a single isomer, is a fluorescent labeling reagent used for labeling peptides, proteins and nucleotides. 5-FAM SE can react with amines and can yield stable amine conjugates[1][2].

In Vitro

The capillary electrophoresis (CE)-laser-induced fluorescent (LIF) method with 5-FAM SE (5-Carboxyfluorescein N-succinimidyl ester) derivatization for the analysis of serotonin can be used to quantify luminally released 5-HT with high sensitivity. CFSE is an excellent dye for the derivatization of 5-HT[1].
5-FAM SE (5-Carboxyfluorescein succinimidyl ester) is chosen as the labeling reagent to precapillary derivatize the two marker aminohydroxyphenylalanine (AHP) isomers produced after reductive hydrolysis of pheomelanin with hydriodic acid (HI)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

473.39

Formula

C₂₅H₁₅NO₉

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (52.81 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1124 mL 10.5621 mL 21.1242 mL
5 mM 0.4225 mL 2.1124 mL 4.2248 mL
10 mM 0.2112 mL 1.0562 mL 2.1124 mL
*Please refer to the solubility information to select the appropriate solvent.
References

Purity: ≥98.0%

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Product Name:
5-FAM SE
Cat. No.:
HY-15938
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