5-DTAF
Based on 1 Customer Validation
5-DTAF is a fluorescein dye that directly react with polysaccharides and other alcohols in aqueous solution at pH ＞9. The maximum of the 5-DTAF emission profile is at a wavelength of 518 nm after excitation at 488 nm. 5-DTAF can be used for biomolecular labeling, protein detection, and cell imaging.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 95.74%
- CAS 番号: 51306-35-5
- 分子式: C23H12Cl2N4O5
- 分子量:495.27
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保管条件:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
5-DTAF (10-5 mol/L; 1 h) acts as a fluorescent competitor for atrazine binding to Fe3O4-chitosan-based MMIP, with supernatant fluorescence intensity increases linearly correlated to the logarithm of atrazine concentration over 2.32-185.4 μM, enabling Atrazine (HY-N7091) detection[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Take cell labeling as an example:
(1) Wash the cells with PBS 3 times to remove serum and culture medium.
(2) Add serum-free medium containing 5-DTAF (1-5 μM), and incubate at 37°C in the dark for 30 minutes.
(3) Discard the staining solution, wash 3 times with preheated medium, and detect with a fluorescence microscope or flow cytometer.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
525
488
化学情報
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CAS 番号 51306-35-5
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性状 Solid
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分子量 495.27
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分子式 C23H12Cl2N4O5
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Color Light yellow to yellow
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SMILES
O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=C(NC6=NC(Cl)=NC(Cl)=N6)C=C5
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
DMSO : 50 mg/mL (100.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (278 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0191 mL | 10.0955 mL | 20.1910 mL | 50.4775 mL |
| 5 mM | 0.4038 mL | 2.0191 mL | 4.0382 mL | 10.0955 mL | |
| 10 mM | 0.2019 mL | 1.0096 mL | 2.0191 mL | 5.0478 mL | |
| 15 mM | 0.1346 mL | 0.6730 mL | 1.3461 mL | 3.3652 mL | |
| 20 mM | 0.1010 mL | 0.5048 mL | 1.0096 mL | 2.5239 mL | |
| 25 mM | 0.0808 mL | 0.4038 mL | 0.8076 mL | 2.0191 mL | |
| 30 mM | 0.0673 mL | 0.3365 mL | 0.6730 mL | 1.6826 mL | |
| 40 mM | 0.0505 mL | 0.2524 mL | 0.5048 mL | 1.2619 mL | |
| 50 mM | 0.0404 mL | 0.2019 mL | 0.4038 mL | 1.0096 mL | |
| 60 mM | 0.0337 mL | 0.1683 mL | 0.3365 mL | 0.8413 mL | |
| 80 mM | 0.0252 mL | 0.1262 mL | 0.2524 mL | 0.6310 mL | |
| 100 mM | 0.0202 mL | 0.1010 mL | 0.2019 mL | 0.5048 mL |
5-DTAF 関連分類
HY-D1082 関連分類
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