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  4. 5-DTAF

5-DTAF is a isomeric fluorescein derivative that directly react with polysaccharides and other alcohols in aqueous solution at pH ＞ 9. The maximum of the 5-DTAF emission profile is at a wavelength of 518 nm after excitation at 488 nm.

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5-DTAF Chemical Structure

5-DTAF Chemical Structure

CAS No. : 51306-35-5

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1 mg USD 30 In-stock
5 mg USD 86 In-stock
10 mg USD 143 In-stock
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Description

5-DTAF is a isomeric fluorescein derivative that directly react with polysaccharides and other alcohols in aqueous solution at pH ＞ 9. The maximum of the 5-DTAF emission profile is at a wavelength of 518 nm after excitation at 488 nm[1].

Molecular Weight

495.27

Formula

C23H12Cl2N4O5
CAS No.
Appearance

Solid

Color

Light yellow to yellow

Emission (Em)

525
Excitation (Ex)

488
SMILES

O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=C(NC6=NC(Cl)=NC(Cl)=N6)C=C5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (100.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0191 mL 10.0955 mL 20.1910 mL
5 mM 0.4038 mL 2.0191 mL 4.0382 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥94.0%

COA (258 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0191 mL 10.0955 mL 20.1910 mL 50.4775 mL
5 mM 0.4038 mL 2.0191 mL 4.0382 mL 10.0955 mL
10 mM 0.2019 mL 1.0096 mL 2.0191 mL 5.0478 mL
15 mM 0.1346 mL 0.6730 mL 1.3461 mL 3.3652 mL
20 mM 0.1010 mL 0.5048 mL 1.0096 mL 2.5239 mL
25 mM 0.0808 mL 0.4038 mL 0.8076 mL 2.0191 mL
30 mM 0.0673 mL 0.3365 mL 0.6730 mL 1.6826 mL
40 mM 0.0505 mL 0.2524 mL 0.5048 mL 1.2619 mL
50 mM 0.0404 mL 0.2019 mL 0.4038 mL 1.0096 mL
60 mM 0.0337 mL 0.1683 mL 0.3365 mL 0.8413 mL
80 mM 0.0252 mL 0.1262 mL 0.2524 mL 0.6310 mL
100 mM 0.0202 mL 0.1010 mL 0.2019 mL 0.5048 mL
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5-DTAF Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-DTAF51306-35-5Fluorescent DyepolysaccharidesfluoresceinalcoholsInhibitorinhibitorinhibit

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