75350-46-8
Chemical Structure
Fluorescein-5-maleimide
Synonym(s): N-(5-Fluoresceinyl)maleimide
- CAS No.: 75350-46-8
- Formula:C24H13NO7
- Molecular Weight:427.36
IUPAC Name: 1-(3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-5-yl)-1H-pyrrole-2,5-dione
InChIKey: AYDAHOIUHVUJHQ-UHFFFAOYSA-N
SMILES: O=C(C=C1)N(C2=CC3=C(C4(C5=C(OC6=C4C=CC(O)=C6)C=C(O)C=C5)OC3=O)C=C2)C1=O
Biological Activity:
Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a fluorescent dye. Fluorescein-5-maleimide can be used to detect the redox state of thiols in eukaryotic cells. Fluorescein-5-maleimide can label peptides and is used to detect negatively charged nanoparticles. Fluorescein-5-maleimide can also label actin to explore its interaction with cardiac myosin-binding protein C (cMyBP-C), which helps in developing small molecule modulators for heart failure. Fluorescein-5-maleimide can screen mutant proteins that contain cysteine residues. The excitation wavelength of Fluorescein-5-maleimide is 494 nm, and the emission wavelength is 519 nm[1][2][3][4].
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Fluorescein-5-maleimide | 99.48% | Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a fluorescent dye. Fluorescein-5-maleimide can be used to detect the redox state of thiols in eukaryotic cells. Fluorescein-5-maleimide can label peptides and is used to detect negatively charged nanoparticles. Fluorescein-5-maleimide can also label actin to explore its interaction with cardiac myosin-binding protein C (cMyBP-C), which helps in developing small molecule modulators for heart failure. Fluorescein-5-maleimide can screen mutant proteins that contain cysteine residues. The excitation wavelength of Fluorescein-5-maleimide is 494 nm, and the emission wavelength is 519 nm. |
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Fluorescein-5-maleimide (solution) | Fluorescein-5-maleimide (solution) (N-(5-Fluoresceinyl)maleimide (solution)) is a fluorescent dye. Fluorescein-5-maleimide can be used to detect the redox state of thiols in eukaryotic cells. Fluorescein-5-maleimide can label peptides and is used to detect negatively charged nanoparticles. Fluorescein-5-maleimide can also label actin to explore its interaction with cardiac myosin-binding protein C (cMyBP-C), which helps in developing small molecule modulators for heart failure. Fluorescein-5-maleimide can screen mutant proteins that contain cysteine residues. The excitation wavelength of Fluorescein-5-maleimide is 494 nm, and the emission wavelength is 519 nm. Solvent and concentration: DMSO: 10 mM |
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References
- [1]. Wadley AJ, et al. Detecting intracellular thiol redox state in leukaemia and heterogeneous immune cell populations: An optimised protocol for digital flow cytometers. MethodsX. 2018 Nov 1;5:1473-1483. [Content Brief]
- [2]. Akinloye O, et al. Peptide-based fluorescence biosensors for detection/measurement of nanoparticles. Anal Bioanal Chem. 2017 Feb;409(4):903-915. [Content Brief]
- [3]. Bunch TA, et al. Drug discovery for heart failure targeting myosin-binding protein C. J Biol Chem. 2023 Dec;299(12):105369. [Content Brief]
- [4]. McLachlin DT, et al. A method of screening for mutant proteins containing cysteine residues using fluorescein-5-maleimide. Protein Expr Purif. 1996 May;7(3):275-80. [Content Brief]
