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Cat. No.: HY-D1402

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5MP-Fluorescein

5MP-Fluorescein (compound 3e) is a fluorescein dye base on 5-Methylene pyrrolone (5MP).5-Methylene pyrrolones are highly thiol-specific and tracelessly removable bioconjugation tools.

For research use only. We do not sell to patients.

CAS No.: 2100282-58-2
Formula: C₂₅H₁₅NO₆
Molecular Weight:425.39

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (275 KB)

5MP-Fluorescein Chemical Structure

CAS No.: 2100282-58-2

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Emission (Em)
- Excitation (Ex)
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Bioz

Biological Activity

Description

5MP-Fluorescein (compound 3e) is a fluorescein dye base on 5-Methylene pyrrolone (5MP).5-Methylene pyrrolones are highly thiol-specific and tracelessly removable bioconjugation tools^[1].

Emission (Em)

525

Excitation (Ex)

488

Chemical Information

CAS No. 2100282-58-2
Molecular Weight 425.39
Formula C₂₅H₁₅NO₆
SMILES

O=C1OC2(C3=CC=C(N4C(C=CC4=C)=O)C=C13)C5=CC=C(C=C5OC6=CC(O)=CC=C62)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Yingqian Zhang, et al. Thiol Specific and Tracelessly Removable Bioconjugation via Michael Addition to 5-Methylene Pyrrolones. J Am Chem Soc. 2017 May 3;139(17):6146-6151. [Content Brief]

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight Molecular Weight

Dilution Calculator

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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