5-Aminofluorescein
Based on 1 Customer Validation
5-Aminofluorescein (5-AF) is a fluorescence marker, covalently bound to human serum albumin. The excitation wavelength is 495 nm and the emission wavelength is 535 nm.
For research use only. We do not sell to patients.
- Purity: 99.52%
- CAS No.: 3326-34-9
- Formula: C20H13NO5
- Molecular Weight:347.33
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
5-Aminofluorescein-HSA : 5-Aminofluorescein is linked to HSA in a 1 : 1 molar ratio to provide a specific and longlasting fluorescence staining of malignant brain tumor.
The excitation wavelength is 495 nm and the emission wavelength is 535 nm.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
515
490
Chemical Information
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CAS No. 3326-34-9
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Appearance Solid
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Molecular Weight 347.33
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Formula C20H13NO5
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Color Brown to reddish brown
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SMILES
O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=C(N)C=C5
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Synonyms
5-AF
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : ≥ 32 mg/mL (92.13 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Ding R, et al. Pharmacokinetics of 5-aminofluorescein-albumin, a novel fluorescence marker of brain tumors during surgery. J Clin Pharmacol. 2011 May;51(5):672-8. [Content Brief]
[2]. Kremer P, et al. Intraoperative fluorescence staining of malignant brain tumors using 5-aminofluorescein-labeled albumin. Neurosurgery. 2009 Mar;64(3 Suppl):ons53-60; discussion ons60-1. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8791 mL | 14.3955 mL | 28.7911 mL | 71.9777 mL |
| 5 mM | 0.5758 mL | 2.8791 mL | 5.7582 mL | 14.3955 mL | |
| 10 mM | 0.2879 mL | 1.4396 mL | 2.8791 mL | 7.1978 mL | |
| 15 mM | 0.1919 mL | 0.9597 mL | 1.9194 mL | 4.7985 mL | |
| 20 mM | 0.1440 mL | 0.7198 mL | 1.4396 mL | 3.5989 mL | |
| 25 mM | 0.1152 mL | 0.5758 mL | 1.1516 mL | 2.8791 mL | |
| 30 mM | 0.0960 mL | 0.4799 mL | 0.9597 mL | 2.3993 mL | |
| 40 mM | 0.0720 mL | 0.3599 mL | 0.7198 mL | 1.7994 mL | |
| 50 mM | 0.0576 mL | 0.2879 mL | 0.5758 mL | 1.4396 mL | |
| 60 mM | 0.0480 mL | 0.2399 mL | 0.4799 mL | 1.1996 mL | |
| 80 mM | 0.0360 mL | 0.1799 mL | 0.3599 mL | 0.8997 mL |
5-Aminofluorescein Related Classifications
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