1. Others
  2. Fluorescent Dye
  3. 5-Aminofluorescein

5-Aminofluorescein  (Synonyms: 5-AF)

Cat. No.: HY-D0022 Purity: 99.79%
COA Handling Instructions

5-Aminofluorescein (5-AF) is a fluorescence marker, covalently bound to human serum albumin. The excitation wavelength is 495 nm and the emission wavelength is 535 nm.

For research use only. We do not sell to patients.

5-Aminofluorescein Chemical Structure

5-Aminofluorescein Chemical Structure

CAS No. : 3326-34-9

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 66 In-stock
Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Solid
500 mg USD 60 In-stock
1 g USD 96 In-stock
5 g   Get quote  
10 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 5-Aminofluorescein:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

5-Aminofluorescein (5-AF) is a fluorescence marker, covalently bound to human serum albumin. The excitation wavelength is 495 nm and the emission wavelength is 535 nm[1].

In Vitro

5-Aminofluorescein-HSA : 5-Aminofluorescein is linked to HSA in a 1 : 1 molar ratio to provide a specific and longlasting fluorescence staining of malignant brain tumor.
The excitation wavelength is 495 nm and the emission wavelength is 535 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

347.32

Formula

C20H13NO5

CAS No.
Appearance

Solid

Color

Light brown to black

Emission (Em)

515

Excitation (Ex)

490

SMILES

O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=C(N)C=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 32 mg/mL (92.13 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8792 mL 14.3959 mL 28.7919 mL
5 mM 0.5758 mL 2.8792 mL 5.7584 mL
10 mM 0.2879 mL 1.4396 mL 2.8792 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation

Purity: 99.79%

References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

5-Aminofluorescein Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
5-Aminofluorescein
Cat. No.:
HY-D0022
Quantity:
MCE Japan Authorized Agent: