6-Aminofluorescein
Based on 1 publication(s) in Google Scholar
6-Aminofluorescein (6-AF) is a fluorescent labeling reagent. 6-Aminofluorescein can be used to label fullerene-based liposome nanostructures termed ‘buckysomes′ (Ex/Em = 490/515 nm).
For research use only. We do not sell to patients.
- Purity: 98.55%
- CAS No.: 51649-83-3
- Formula: C20H13NO5
- Molecular Weight:347.32
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 6-Aminofluorescein
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Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Buckysomes are coupled to 6-Aminofluorescein
400 μL of buckysomes (2 mg/mL) is incubated with 100 μL each of 0.25 M EDC (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (HY-D0178) and 0.25 M sulfo-NHS for 2 hrs at room temperature.
The pH is adjusted to 7.0 using NaOH.
300 μL of 6-Aminofluorescein (1 mg/mL prepared in DMSO) is added and incubated overnight at room temperature.
The free 6-Aminofluorescein is separated from 6-Aminofluorescein coupled amphiphilic fullerene monomer (AF-1) by size exclusion chromatography on Cross-linked dextran G 75 (HY-148139) column.
The fractions are analyzed by fluorometry for 6-Aminofluorescein emission at 520 nm.
Localization of 6-Aminofluorescein conjugated AF-1 using Fluorescence Microscopy
Human Coronary Artery Endothelial Cells are grown in 8-chamber tissue culture slides.
Exposed to 6-Aminofluorescein-buckysomes for 18 hrs at 37°C, 5%CO2.
Washes with Dulbecco's phosphate buffered saline.
Cells are fixed in 4% Paraformaldehyde for 20 min, and washed twice with Dulbecco's phosphate buffered saline.
Chambers are removed and slides are dried.
Fixed cells are mounted in antifade reagent with DAPI.
Observe using a fluorescence microscope.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
515
490
Chemical Information
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CAS No. 51649-83-3
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Appearance Solid
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Molecular Weight 347.32
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Formula C20H13NO5
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Color Yellow to orange
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SMILES
O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=CC(N)=C5
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Synonyms
6-AF
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
DMSO : 100 mg/mL (287.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (279 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8792 mL | 14.3959 mL | 28.7919 mL | 71.9797 mL |
| 5 mM | 0.5758 mL | 2.8792 mL | 5.7584 mL | 14.3959 mL | |
| 10 mM | 0.2879 mL | 1.4396 mL | 2.8792 mL | 7.1980 mL | |
| 15 mM | 0.1919 mL | 0.9597 mL | 1.9195 mL | 4.7986 mL | |
| 20 mM | 0.1440 mL | 0.7198 mL | 1.4396 mL | 3.5990 mL | |
| 25 mM | 0.1152 mL | 0.5758 mL | 1.1517 mL | 2.8792 mL | |
| 30 mM | 0.0960 mL | 0.4799 mL | 0.9597 mL | 2.3993 mL | |
| 40 mM | 0.0720 mL | 0.3599 mL | 0.7198 mL | 1.7995 mL | |
| 50 mM | 0.0576 mL | 0.2879 mL | 0.5758 mL | 1.4396 mL | |
| 60 mM | 0.0480 mL | 0.2399 mL | 0.4799 mL | 1.1997 mL | |
| 80 mM | 0.0360 mL | 0.1799 mL | 0.3599 mL | 0.8997 mL | |
| 100 mM | 0.0288 mL | 0.1440 mL | 0.2879 mL | 0.7198 mL |
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