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  4. ACE TFA

ACE TFA is a low-background, photostable fluorophore with nanomolar binding affinity for the Okra RNA aptamer. ACE TFA enables clear visualization of mRNA in live bacteria and mammalian cells, including tracking the trafficking of mRNA to stress granules and dual-color super-resolution imaging of RNA in live cells. (Ex=488 nm, Em=555 nm).

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ACE TFA

ACE TFA Chemical Structure

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 mg In-stock
5 mg In-stock
10 mg In-stock
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Other Forms of ACE TFA:

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ACE TFA Related Antibodies

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  • Purity & Documentation

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Description

ACE TFA is a low-background, photostable fluorophore with nanomolar binding affinity for the Okra RNA aptamer. ACE TFA enables clear visualization of mRNA in live bacteria and mammalian cells, including tracking the trafficking of mRNA to stress granules and dual-color super-resolution imaging of RNA in live cells. (Ex=488 nm, Em=555 nm)[1].

In Vitro

ACE TFA (10 nM-10 μM; 3-10 min) forms a highly stable, bright green fluorescent complex with the Okra RNA aptamer, exhibiting nanomolar binding affinity, high quantum yield, low magnesium dependence, potassium independence, and pH stability in vitro[1].
ACE TFA (0.5-2 μM; 10 min at 37°C, up to 1200 s) produces significantly brighter green fluorescence than reference fluorogenic ligands in live HEK293T cells, with exceptional photostability exceeding that of EGFP during long-term continuous imaging when bound to Okra[1].
ACE TFA (0.5 μM; 10 min at 37°C) enables real-time, long-term imaging of mRNA trafficking to and dynamics of stress granules in live HeLa cells when bound to Okra-tagged ACTB mRNA[1].
ACE TFA (1 μM; 10 min at 37°C) supports high-resolution live-cell SIM imaging of subcellular RNA localization, including detailed visualization of RNA distributions in stress granules when bound to the Okra RNA aptamer[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ACE TFA Related Antibodies
Molecular Weight

468.47

Formula

C22H27F3N4O4
Appearance

Solid

SMILES

O=C(/C(C#N)=C\C1=CC2=C(N(CCC2)CCC3)C3=C1)NCCOCCN.OC(C(F)(F)F)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (213.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1346 mL 10.6730 mL 21.3461 mL
5 mM 0.4269 mL 2.1346 mL 4.2692 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.47%

SDS (252 KB)

COA (251 KB) HNMR (267 KB) LCMS (98 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1346 mL 10.6730 mL 21.3461 mL 53.3652 mL
5 mM 0.4269 mL 2.1346 mL 4.2692 mL 10.6730 mL
10 mM 0.2135 mL 1.0673 mL 2.1346 mL 5.3365 mL
15 mM 0.1423 mL 0.7115 mL 1.4231 mL 3.5577 mL
20 mM 0.1067 mL 0.5337 mL 1.0673 mL 2.6683 mL
25 mM 0.0854 mL 0.4269 mL 0.8538 mL 2.1346 mL
30 mM 0.0712 mL 0.3558 mL 0.7115 mL 1.7788 mL
40 mM 0.0534 mL 0.2668 mL 0.5337 mL 1.3341 mL
50 mM 0.0427 mL 0.2135 mL 0.4269 mL 1.0673 mL
60 mM 0.0356 mL 0.1779 mL 0.3558 mL 0.8894 mL
80 mM 0.0267 mL 0.1334 mL 0.2668 mL 0.6671 mL
100 mM 0.0213 mL 0.1067 mL 0.2135 mL 0.5337 mL
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ACE TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ACEFluorescent DyeFluorescent dyesRNA aptamersLiving cellsvisualizationInhibitorinhibitorinhibit

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