ACE

ACE (10 nM-10 μM; 3-10 min) forms a highly stable, bright green fluorescent complex with the Okra RNA aptamer, exhibiting nanomolar binding affinity, high quantum yield, low magnesium dependence, potassium independence, and pH stability in vitro^[1].
ACE (0.5-2 μM; 10 min at 37°C, up to 1200 s) produces significantly brighter green fluorescence than reference fluorogenic ligands in live HEK293T cells, with exceptional photostability exceeding that of EGFP during long-term continuous imaging when bound to Okra^[1].
ACE (0.5 μM; 10 min at 37°C) enables real-time, long-term imaging of mRNA trafficking to and dynamics of stress granules in live HeLa cells when bound to Okra-tagged ACTB mRNA^[1].
ACE (1 μM; 10 min at 37°C) supports high-resolution live-cell SIM imaging of subcellular RNA localization, including detailed visualization of RNA distributions in stress granules when bound to the Okra RNA aptamer^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.