ACE 

ACE is a low-background, photostable fluorophore with nanomolar binding affinity for the Okra RNA aptamer. ACE enables clear visualization of mRNA in live bacteria and mammalian cells, including tracking the trafficking of mRNA to stress granules and dual-color super-resolution imaging of RNA in live cells. (Ex=488 nm, Em=555 nm)^[1].

ACE (10 nM-10 μM; 3-10 min) forms a highly stable, bright green fluorescent complex with the Okra RNA aptamer, exhibiting nanomolar binding affinity, high quantum yield, low magnesium dependence, potassium independence, and pH stability in vitro^[1].
ACE (0.5-2 μM; 10 min at 37°C, up to 1200 s) produces significantly brighter green fluorescence than reference fluorogenic ligands in live HEK293T cells, with exceptional photostability exceeding that of EGFP during long-term continuous imaging when bound to Okra^[1].
ACE (0.5 μM; 10 min at 37°C) enables real-time, long-term imaging of mRNA trafficking to and dynamics of stress granules in live HeLa cells when bound to Okra-tagged ACTB mRNA^[1].
ACE (1 μM; 10 min at 37°C) supports high-resolution live-cell SIM imaging of subcellular RNA localization, including detailed visualization of RNA distributions in stress granules when bound to the Okra RNA aptamer^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

354.45

C₂₀H₂₆N₄O₂

3058751-62-2

O=C(/C(C#N)=C\C1=CC2=C(N(CCC2)CCC3)C3=C1)NCCOCCN

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Zuo F, et al. Imaging the dynamics of messenger RNA with a bright and stable green fluorescent RNA. Nat Chem Biol. 2024;20(10):1272-1281.  [Content Brief]