The Influence of Extracellular Vesicles Secreted by Dural Cells on Osteoblasts

  • Mol Biotechnol. 2023 Dec 1. doi: 10.1007/s12033-023-00974-x.
Fangning Zhao  1 ,  Jinglin Zhu  2 ,  Xinhang Dong  1 ,  Xiaoshuang Guo  1 ,  Chenzhi Lai  1 ,  Jingyi Zhao  1 ,  Xianlei Zong  1 ,  Guodong Song  1 ,  Xiaolei Jin  3
Affiliations
  • 1. The 4th Craniomaxillofacial Department, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, 33 Badachu Road, Shijingshan District, Beijing, 100144, China.
  • 2. The Adipose Remodeling Department, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, 33 Badachu Road, Shijingshan District, Beijing, 100144, China.
  • 3. The 4th Craniomaxillofacial Department, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, 33 Badachu Road, Shijingshan District, Beijing, 100144, China. [email protected].
PMID: 38040933 DOI: 10.1007/s12033-023-00974-x
Abstract

To explore the influence of extracellular vesicles secreted by dural cells (Dura-EVs) on osteoblasts. Our methodology involves assessing the effects of these EVs at concentrations of 50ug/ml, 100ug/ml, and 200ug/ml on osteoblasts proliferation, differentiation, migration, osteogenesis, and inhibition of Apoptosis. We also treated a cranial defect model with injections of these Dura-EVs and monitored the healing rate of cranial defects. Tissue sections were analyzed using Hematoxylin and Eosin (H & E), Masson's trichrome, and immunofluorescence (IF) staining. Our results suggest that Dura-EVs can enhance osteoblasts proliferation, migration, differentiation, and osteogenesis in a dose-dependent manner in vitro. In vivo, Dura-EVs may promote the repair of skull defects. Dura-EVs have an important influence on osteoblasts, our findings shed light on a novel aspect of the dura mater's contribution to cranial osteogenesis.

Keywords
Dural cells; Exosomes; Extracellular vesicles; Osteoblasts.
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