1. Membrane Transporter/Ion Channel Apoptosis
  2. P-glycoprotein Apoptosis
  3. Solamargine

Solamargine  (Synonyms: Solamargin; δ-Solanigrine)

Cat. No.: HY-N0069 Purity: 99.97%
COA Handling Instructions

Solamargine, a derivative from the steroidal solasodine in Solanum species, exhibits anticancer activities in numerous types of cancer. Solamargine induces non-selective cytotoxicity and P-glycoprotein inhibition. Solamargine significantly inhibits migration and invasion of HepG2 cells by down-regulating MMP-2 and MMP-9 expression and activity.

For research use only. We do not sell to patients.

Solamargine Chemical Structure

Solamargine Chemical Structure

CAS No. : 20311-51-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
USD 206 In-stock
Solution
10 mM * 1 mL in DMSO USD 206 In-stock
Solid
1 mg USD 68 In-stock
5 mg USD 144 In-stock
10 mg USD 216 In-stock
50 mg USD 816 In-stock
100 mg   Get quote  
200 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 12 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Solamargine purchased from MedChemExpress. Usage Cited in: Int J Oncol. 2019 Mar;54(3):905-915.   [Abstract]

    Western blot analysis for the protein expression of XIAP, survivin, Ki‑67, PCNA and cyclin D1 in the treatment of MOCK, solamargine and Fe3O4-solamargine.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Solamargine, a derivative from the steroidal solasodine in Solanum species, exhibits anticancer activities in numerous types of cancer. Solamargine induces non-selective cytotoxicity and P-glycoprotein inhibition. Solamargine significantly inhibits migration and invasion of HepG2 cells by down-regulating MMP-2 and MMP-9 expression and activity[1][2].

    In Vitro

    Solamargine (15 μg/ml; 72 hours) predominantly stimulates the cells of the G2/M phase to apoptosis[3].
    Solamargine (72 hours) dramatically inhibits the proliferation of SMMC-7721 and HepG2 cells in a dose- and time-dependent manner. The IC50s are 9.21 and 19.88 μg/ml, respectively[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[3]

    Cell Line: SMMC-7721cells
    Concentration: 15 μg/ml
    Incubation Time: 72 hours
    Result: Displayed a significant increase of sub-G1.
    In Vivo

    Solamargine (10 mg/kg; intragastric administration; once daily for 8 days) exhibits an antitumor effect and promotes the apoptosis of GC in vivo[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female specific pathogen-free BALB/c nude mice weighing 18-20 g (6-8-weeks-old)[4]
    Dosage: 10 mg/kg
    Administration: Intragastric administration; once daily for 8 days
    Result: Tumor growth was significantly inhibited .
    Molecular Weight

    868.06

    Formula

    C45H73NO15

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@H](CC1)CN[C@]21O[C@@]3([H])C[C@@]4([H])[C@]5([H])CC=C6C[C@@H](O[C@@]7([H])[C@H](O[C@@]8([H])[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O8)[C@@H](O)[C@H](O[C@]9([H])O[C@@H](C)[C@H](O)[C@@H](O)[C@H]9O)[C@@H](CO)O7)CC[C@]6(C)[C@@]5([H])CC[C@]4(C)[C@@]3([H])[C@@H]2C

    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (115.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.1520 mL 5.7600 mL 11.5199 mL
    5 mM 0.2304 mL 1.1520 mL 2.3040 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

    =
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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (2.88 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (2.88 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.97%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.1520 mL 5.7600 mL 11.5199 mL 28.7999 mL
    5 mM 0.2304 mL 1.1520 mL 2.3040 mL 5.7600 mL
    10 mM 0.1152 mL 0.5760 mL 1.1520 mL 2.8800 mL
    15 mM 0.0768 mL 0.3840 mL 0.7680 mL 1.9200 mL
    20 mM 0.0576 mL 0.2880 mL 0.5760 mL 1.4400 mL
    25 mM 0.0461 mL 0.2304 mL 0.4608 mL 1.1520 mL
    30 mM 0.0384 mL 0.1920 mL 0.3840 mL 0.9600 mL
    40 mM 0.0288 mL 0.1440 mL 0.2880 mL 0.7200 mL
    50 mM 0.0230 mL 0.1152 mL 0.2304 mL 0.5760 mL
    60 mM 0.0192 mL 0.0960 mL 0.1920 mL 0.4800 mL
    80 mM 0.0144 mL 0.0720 mL 0.1440 mL 0.3600 mL
    100 mM 0.0115 mL 0.0576 mL 0.1152 mL 0.2880 mL
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Solamargine
    Cat. No.:
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