2. Apoptosis
  3. Caspase
  4. Ac-IETD-AFC

Ac-IETD-AFC is a fluorogenic substrate of caspase-8, caspase-3, caspase-10, and granzyme B.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Ac-IETD-AFC Chemical Structure

Ac-IETD-AFC Chemical Structure

CAS No. : 211990-57-7

Size Price Stock Quantity
1 mg USD 80 In-stock
5 mg USD 230 In-stock
10 mg USD 360 In-stock
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100 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

Ac-IETD-AFC Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Ac-IETD-AFC

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Description

Ac-IETD-AFC is a fluorogenic substrate of caspase-8, caspase-3, caspase-10, and granzyme B[1].

Molecular Weight

729.65

Formula

C31H38F3N5O12
CAS No.
Appearance

Solid

Color

White to off-white

Emission (Em)

500
Excitation (Ex)

380
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (171.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3705 mL 6.8526 mL 13.7052 mL
5 mM 0.2741 mL 1.3705 mL 2.7410 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3705 mL 6.8526 mL 13.7052 mL 34.2630 mL
5 mM 0.2741 mL 1.3705 mL 2.7410 mL 6.8526 mL
10 mM 0.1371 mL 0.6853 mL 1.3705 mL 3.4263 mL
15 mM 0.0914 mL 0.4568 mL 0.9137 mL 2.2842 mL
20 mM 0.0685 mL 0.3426 mL 0.6853 mL 1.7132 mL
25 mM 0.0548 mL 0.2741 mL 0.5482 mL 1.3705 mL
30 mM 0.0457 mL 0.2284 mL 0.4568 mL 1.1421 mL
40 mM 0.0343 mL 0.1713 mL 0.3426 mL 0.8566 mL
50 mM 0.0274 mL 0.1371 mL 0.2741 mL 0.6853 mL
60 mM 0.0228 mL 0.1142 mL 0.2284 mL 0.5711 mL
80 mM 0.0171 mL 0.0857 mL 0.1713 mL 0.4283 mL
100 mM 0.0137 mL 0.0685 mL 0.1371 mL 0.3426 mL
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Ac-IETD-AFC Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ac-IETD-AFC211990-57-7Caspasefluorogenic substratecaspase-8caspase-3caspase-10granzyme BInhibitorinhibitorinhibit

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