M-CSF Antibody

Cat. No.: HY-P80750: FAQs
Data Sheet SDS COA User Guide for Antibodies

M-CSF Antibody is a non-conjugated and Rabbit origined monoclonal antibody, targeting to M-CSF. It can be used for WB,IHC-F,IHC-P,ICC/IF assays with tag free, in the background of Human.

For research use only. We do not sell to patients.

Size	Price	Stock	Quantity
10 μL	$70	In-stock	Estimated Time of Arrival: December 31
50 μL	$185	In-stock	Estimated Time of Arrival: December 31
100 μL	$300	In-stock	Estimated Time of Arrival: December 31
250 μL		Get quote

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Biological Activity
Technical Parameters
Properties
Documentation

Description	M-CSF Antibody is a non-conjugated and Rabbit origined monoclonal antibody, targeting to M-CSF. It can be used for WB,IHC-F,IHC-P,ICC/IF assays with tag free, in the background of Human.
Background	M-CSF: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
Tag	Free
Gene ID	1435
Synonyms	CSF1; Macrophage colony-stimulating factor 1; CSF-1; M-CSF; MCSF; Lanimostim
Molecular Weight	Predicted band size: 43 kDa
Purity	affinity purified
Conjugation	Non-conjugated
Research Field	Immunology
Product Categories	Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Host	Rabbit
Reactivity	Human
SwissProt ID	P09603
Dilution Ratio	WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200 Western blot analysis of extracts from Jurkat(lane 2(20μg) or lane 3(40μg) ) and K562(lane 4(20μg) or lane 5(40μg)) using M-CSF(HY-P80750) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80750, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.
Application	WB, IHC-F, IHC-P, ICC/IF
Appearance	Liquid
Formulation	Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Immunogen	Synthetic peptide corresponding to Human M-CSF.The exact sequence is proprietary to MCE.
Documentation	Select Batch: Data Sheet (234 KB) SDS (251 KB) COA (205 KB) User Guide for Antibodies (1077 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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M-CSF Antibody

Biological Activity

Technical Parameters

Properties

Documentation

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