2. Anti-infection Membrane Transporter/Ion Channel Apoptosis
  3. Bacterial Fungal Na+/K+ ATPase Apoptosis Antibiotic
  4. Bafilomycin C1

Bafilomycin C1 is a macrolide antibiotic isolated from Streptomyces sp. Bafilomycin C1 is a potent, specific and reversible inhibitor of vacuolar-type H+-ATPases (V-ATPases). Bafilomycin C1 inhibits growth of gram-positive bacteria and fungi. Bafilomycin C1 induces cell apoptosis and can be used for the study of hepatocellular carcinoma (HCC).

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Bafilomycin C1 Chemical Structure

Bafilomycin C1 Chemical Structure

CAS No. : 88979-61-7

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Bafilomycin C1 Related Antibodies

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Description

Bafilomycin C1 is a macrolide antibiotic isolated from Streptomyces sp. Bafilomycin C1 is a potent, specific and reversible inhibitor of vacuolar-type H+-ATPases (V-ATPases). Bafilomycin C1 inhibits growth of gram-positive bacteria and fungi[2]. Bafilomycin C1 induces cell apoptosis and can be used for the study of hepatocellular carcinoma (HCC)[2].

IC50 & Target

Macrolide

 

In Vitro

Bafilomycin C1 (0.33-10 μM; 6 days) inhibits the growth and proliferation of SMMC7721 and HepG2 cells in a timeand dose-dependent manner[2].
Bafilomycin C1 (0.33-3.3 μM; 24 hours) decreases cyclin D3, cyclin E1, CDK2, CDK4, and CDK6 expression in both mRNA and protein expression in SMMC7721 cells[2].
Bafilomycin C1 (3.3-10 μM; 24 hours) causes morphological alterations and increases the population of apoptotic cells by Hoechst 33258 (HY-15558) staining compared to vehicle[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Bafilomycin C1 Related Antibodies

Cell Viability Assay[2]

Cell Line: SMMC7721 cell and HepG2 cell
Concentration: 0.33 μM, 1.1 μM, and 3.3 μM for SMMC7721
1.1 μM, 3.3 μM, and 10.0 μM for HepG2
Incubation Time: 6 days
Result: Retarded the cell growth.

Western Blot Analysis[2]

Cell Line: SMMC7721 cells
Concentration: 3.3 μM
Incubation Time: 24 hours
Result: Decreaed cyclin D3/E1,CDK2/4/6 protein expression and increased p21.

Apoptosis Analysis[2]

Cell Line: SMMC7721 and HepG2 cells
Concentration: 3.3 μM; 10 μM
Incubation Time: 24 hours
Result: Induced apoptosis in SMMC7721 and HepG2 cells.
In Vivo

Bafilomycin C1 (subcutaneous injection; 0.2 mg/kg; 20 days) retards the tumor growth without apparent adverse reactions or side effects in nude mice model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c nude mice (weighing 18-20 g) subcutaneous injected by SMMC7721 cell suspension (5×106 cells/100 μL)[2]
Dosage: 0.2 mg/kg
Administration: Subcutaneous injection; 20 days
Result: Suppressed tumor growth of SMMC7721 tumor xenografts.
Molecular Weight

720.89

Formula

C39H60O12
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@H]([C@@](C[C@H]([C@@H]1C)OC(/C=C/C(O)=O)=O)(O[C@@H]1C(C)C)O)[C@H](O)[C@@H]([C@](OC(/C(OC)=C/C(C)=C/[C@H]2C)=O)([H])[C@H](/C=C/C=C(C[C@@H]([C@@H]2O)C)\C)OC)C
Structure Classification
Initial Source

Streptomyces sp
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (6.94 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3872 mL 6.9359 mL 13.8717 mL
5 mM 0.2774 mL 1.3872 mL 2.7743 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

The following protocol is derived from the literature and is for reference only. It is recommended to first try a small sample.

  • Protocol 1

    Bafilomycin C1 (0.2 mg/kg body weight, dissolved in 60% 1,2-propanediol)[2]

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥99.0%

COA (250 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3872 mL 6.9359 mL 13.8717 mL 34.6794 mL
5 mM 0.2774 mL 1.3872 mL 2.7743 mL 6.9359 mL
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Bafilomycin C1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bafilomycin C188979-61-7Bafilomycin C 1Bafilomycin C-1BacterialFungalNa+/K+ ATPaseApoptosisAntibioticSodium potassium pumpprokaryoticeukaryoticSMMC7721HepatocellularcarcinomaHCChepaticchemotherapyInhibitorinhibitorinhibit

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