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  3. BTBCT

BTBCT is mainly used as a label in time-resolved fluorescence immunoassays (TRFIA). The lower limit of detection for TSH TR-IFMA is 0.011 mIU/L in a 10 μl sample volume. The high fluorescence intensity and stability of BTBCT improves the sensitivity of the assay.

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BTBCT Chemical Structure

BTBCT Chemical Structure

CAS No. : 525560-81-0

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Description

BTBCT is mainly used as a label in time-resolved fluorescence immunoassays (TRFIA). The lower limit of detection for TSH TR-IFMA is 0.011 mIU/L in a 10 μl sample volume. The high fluorescence intensity and stability of BTBCT improves the sensitivity of the assay[1].

In Vitro

Protein labeling with BTBCT
1:Dissolve BTBCT at a concentration of 10 mg/mL in dry ethanol.
2:Dissolve the target protein (e.g., BSA or streptavidin) in 0.1 M sodium carbonate buffer to the desired concentration, typically 1 mg/mL.
3:Gradually add the BTBCT solution to the protein solution while stirring.
4:Maintain stirring at room temperature for 2 hours.
5:Filter the mixture using an appropriate size filter (usually 0.2 µm).
6:If necessary, further purify the labeled protein via affinity chromatography to ensure that only functionalized protein is collected.
7:Dialyze the purified protein against a suitable storage buffer (commonly containing 0.05% NaN3).
8:Store at 4°C or freeze as needed.

Indirect Serum TSH TR-IFMA Procedure:
1: Coating the Microplate: Add 30 µL of coating buffer containing 15 µg/mL of anti-TSH McAb-05 to each well. Incubate at room temperature for 24 hours. Wash twice with wash buffer.
2: Blocking: Add 40 µL of blocking buffer (typically containing BSA or another protein) and incubate at room temperature for 6 hours. Wash and air dry.
3:Adding Samples and Standards: Add 10 µL of TSH standard or serum sample to be tested to each well. Add 10 µL of a mixture containing biotinylated anti-TSH McAb-04 and McAb-03 at a concentration of about 30 ng/µL. Incubate with gentle shaking at room temperature for 1 hour.
4:Washing: Thoroughly wash the microplate with wash buffer.
Adding Signal Generation Reagent: Add 20 µL of TSH assay buffer containing streptavidin-BSA-BTBCT-Eu complex to each well. Gently shake and incubate for another 20 minutes.
5: Final Washing: Wash four times and rinse twice with distilled water.
6: Fluorescence Measurement: Measure the fluorescence intensity of each well using a time-resolved fluorometer.
Direct Serum T4 TRFIA Procedure: 1: Microplate Preparation: Use a microplate coated with anti-T4 antibody, typically at a concentration of 10 µg/mL. Incubate the coated antibody at 4°C overnight.
2: Blocking Non-Specific Sites: Block the microplate with blocking buffer (usually containing 1% BSA) at room temperature for 1-2 hours.
3: Adding Samples and Label: Add a predetermined amount of the labeled T4-BSA-BTBCT-Eu complex along with the serum sample to be tested or T4 standard to each well.
4: Competition Reaction: Incubate the plate at room temperature for 1-2 hours.
5: Washing: Thoroughly wash the microplate with wash buffer.
6: Fluorescence Measurement: Measure the fluorescence signal of each well using a time-resolved fluorometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

604.90

Formula

C26H15ClF6O6S

CAS No.
SMILES

O=C(CC(C(F)(F)F)=O)C1=CC=C(C2=CC=C(S(=O)(Cl)=O)C=C2C3=CC=C(C(CC(C(F)(F)F)=O)=O)C=C3)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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BTBCT
Cat. No.:
HY-D0038
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