D-Ala-Lys-AMCA hydrochloride

Name: D-Ala-Lys-AMCA hydrochloride
Brand: MedChemExpress
SKU: HY-111956B
Rating: 4.5 (1 reviews)

Cat. No.: HY-111956B Purity: 98.88%: FAQs
Data Sheet SDS COA Handling Instructions

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D-Ala-Lys-AMCA hydrochloride is a known proton-coupled oligopeptide transporter 1 (PEPT1) substrate that emits blue fluorescence. D-Ala-Lys-AMCA hydrochloride may be transported into liver cancer cells and Caco-2 cells based on fluorescence analysis. D-Ala-Lys-AMCA hydrochloride can be used for characterizing PEPT1-specific substrates or inhibitors.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

D-Ala-Lys-AMCA hydrochloride Chemical Structure

CAS No. : 2703746-41-0

Size	Price	Stock	Quantity
5 mg	USD 380	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 650	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1300	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2200	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 3600	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of D-Ala-Lys-AMCA hydrochloride:

D-Ala-Lys-AMCA Get quote
D-Ala-Lys-AMCA TFA Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

D-Ala-Lys-AMCA (25, 50 and 150 μM, 3 h) hydrochloride may be transported into liver cancer and Caco-2 cells^[1].
D-Ala-Lys-AMCA (25 μM, 10 min) hydrochloride can be uptaken by absorptive enterocytes of all small intestine segments, whereas there is a complete lack of fluorescence in colonic samples^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

468.93

Formula

C₂₁H₂₉ClN₄O₆

CAS No.

2703746-41-0

Appearance

Solid

Color

Light yellow to yellow

Emission (Em)

480

Excitation (Ex)

390

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (213.25 mM; Need ultrasonic)

DMSO : 100 mg/mL (213.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1325 mL	10.6626 mL	21.3251 mL
5 mM	0.4265 mL	2.1325 mL	4.2650 mL
10 mM	0.2133 mL	1.0663 mL	2.1325 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.60%

Select Batch:

Data Sheet (271 KB) SDS (252 KB)

COA (247 KB) HNMR (211 KB) LCMS (119 KB) Elemental Analysis Report (106 KB)

Handling Instructions (2659 KB)

References

[1]. Gong Y, et al. Specific expression of proton-coupled oligopeptide transporter 1 in primary hepatocarcinoma-anovel strategy for tumor-targeted therapy. Oncol Lett. 2017 Oct;14(4):4158-4166. [Content Brief]

[2]. Groneberg DA, et al. Intestinal peptide transport: ex vivo uptake studies and localization of peptide carrier PEPT1. Am J Physiol Gastrointest Liver Physiol. 2001 Sep;281(3):G697-704. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.1325 mL	10.6626 mL	21.3251 mL	53.3129 mL
	5 mM	0.4265 mL	2.1325 mL	4.2650 mL	10.6626 mL
	10 mM	0.2133 mL	1.0663 mL	2.1325 mL	5.3313 mL
	15 mM	0.1422 mL	0.7108 mL	1.4217 mL	3.5542 mL
	20 mM	0.1066 mL	0.5331 mL	1.0663 mL	2.6656 mL
	25 mM	0.0853 mL	0.4265 mL	0.8530 mL	2.1325 mL
	30 mM	0.0711 mL	0.3554 mL	0.7108 mL	1.7771 mL
	40 mM	0.0533 mL	0.2666 mL	0.5331 mL	1.3328 mL
	50 mM	0.0427 mL	0.2133 mL	0.4265 mL	1.0663 mL
	60 mM	0.0355 mL	0.1777 mL	0.3554 mL	0.8885 mL
	80 mM	0.0267 mL	0.1333 mL	0.2666 mL	0.6664 mL
	100 mM	0.0213 mL	0.1066 mL	0.2133 mL	0.5331 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.