2. Academic Validation
  3. AAF-cmk sensitizes tumor cells to trail-mediated apoptosis

AAF-cmk sensitizes tumor cells to trail-mediated apoptosis

  • Leuk Res. 2004 Jan;28(1):53-61. doi: 10.1016/s0145-2126(03)00122-x.
Izabela Młnarczuk 1,  Paweł Mróz,  Grazyna Hoser,  Dominika Nowis,  Łukasz P Biały,  Halina Ziemba,  Tomasz Grzela,  Wojciech Feleszko,  Jacek Malejczyk,  Cezary Wójcik,  Marek Jakóbisiak,  Jakub Gołab
Affiliations

Affiliation

  • 1 Department of Immunology, Center of Biostructure, The Medical University of Warsaw, Chalubińskiego 5, 02004 Warsaw, Poland.
PMID: 14630081 DOI: 10.1016/s0145-2126(03)00122-x
Abstract

TRAIL is a member of the tumor necrosis factor (TNF) superfamily. This cytokine is cytotoxic for a high proportion of tumor cells, but could be also toxic for normal cells. There is a need to find other agents able to potentiate the antitumor effects of this cytokine. In our study, we found that Ala-Ala-Phe-chloromethylketone (AAF-cmk) augmented cytotoxic activity of TRAIL or TNF against human leukemic cells. Flow cytometry studies and electron microscopy revealed that Apoptosis was primarily responsible for this potentiation. Altogether, our studies indicate that AAF-cmk might effectively sensitize human leukemia cells to Apoptosis induced by TRAIL and TNF.

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