2. Academic Validation
  3. Two new triterpenoids from the seeds of blackberry (Rubus fructicosus)

Two new triterpenoids from the seeds of blackberry (Rubus fructicosus)

  • Nat Prod Res. 2016;30(8):904-11. doi: 10.1080/14786419.2015.1076820.
Masateru Ono 1,  Shin Yasuda 1,  Kaori Nishi 1,  Kazutaka Yamamoto 1,  Satoshi Fuchizaki 1,  Satomi Higuchi 1,  Haruki Komatsu 1,  Masafumi Okawa 2,  Junei Kinjo 2,  Hitoshi Yoshimitsu 3,  Toshihiro Nohara 3
Affiliations

Affiliations

  • 1 a Department of Bioscience, School of Agriculture , Tokai University , Kumamoto , Japan.
  • 2 b Faculty of Pharmaceutical Sciences , Fukuoka University , Fukuoka , Japan.
  • 3 c Faculty of Pharmaceutical Sciences , Sojo University , Kumamoto , Japan.
PMID: 26305695 DOI: 10.1080/14786419.2015.1076820
Abstract

Two new ursane-type triterpenoids (1, 2) attached to isopropylidenedioxy group were isolated from the seeds of blackberry (Rubus fructicosus L., Rosaceae) along with two known ursane-type triterpenoids, 2,3-O-isopropylidenyl-2α,3α,19α-trihydroxyurs-12-en-28-oic acid (3) and 1β-hydroxyeuscaphic acid (4). The chemical structures of 1 and 2 were determined to be 2,3-O-isopropylidene-1β,2β,3β,19α-tetrahydroxyurs-12-en-28-oic acid and 1,2-O-isopropylidene-1β,2α,3α,19α-tetrahydroxyurs-12-en-28-oic acid, respectively, based on spectroscopic data. Additionally, their cytotoxic activity towards HL-60 human leukaemia cells was evaluated. Among them, 3 demonstrated a clear cytotoxic activity with 72.8 μM of IC50 value.

Keywords

Rosaceae; Rubus fructicosus; blackberry; cytotoxic activity; isopropylidenedioxy; ursane-type triterpenoid.

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