2. Academic Validation
  3. Probing the biophysical interaction between Neocarzinostatin toxin and EpCAM RNA aptamer

Probing the biophysical interaction between Neocarzinostatin toxin and EpCAM RNA aptamer

  • Biochem Biophys Res Commun. 2016 Jan 8;469(2):257-62. doi: 10.1016/j.bbrc.2015.11.109.
Prasanna Kumar Athyala 1,  Jagat Rakesh Kanwar 2,  Mohamed Alameen 3,  Rupinder Kaur Kanwar 2,  Subramanian Krishnakumar 4,  Jon Watson 2,  Umashankar Vetrivel 3,  Janakiraman Narayanan 5
Affiliations

Affiliations

  • 1 Department of Nanobiotechnology, Vision Research Foundation, Chennai, India; Nanomedicine-Laboratory of Immunology and Molecular Biomedical Research (NLIMBR), School of Medicine (SoM), Centre for Molecular and Medical Research (C-MMR), Faculty of Health, Deakin University, Geelong, Pigdons Road, Waurn Ponds, Geelong, Victoria 3217, Australia; Graduate Student Deakin University (ID: 213510168), Nanomedicine-Laboratory of Immunology and Molecular Biomedical Research (NLIMBR), School of Medicine, Deakin University, Australia.
  • 2 Nanomedicine-Laboratory of Immunology and Molecular Biomedical Research (NLIMBR), School of Medicine (SoM), Centre for Molecular and Medical Research (C-MMR), Faculty of Health, Deakin University, Geelong, Pigdons Road, Waurn Ponds, Geelong, Victoria 3217, Australia.
  • 3 Bioinformatics Centre, Vision Research Foundation, Chennai, India.
  • 4 Department of Nanobiotechnology, Vision Research Foundation, Chennai, India.
  • 5 Department of Nanobiotechnology, Vision Research Foundation, Chennai, India. Electronic address: [email protected].
PMID: 26642954 DOI: 10.1016/j.bbrc.2015.11.109
Abstract

Neocarzinostatin (NCS) a potent DNA-damaging, anti-tumor toxin extracted from Streptomyces carzinostaticus that recognizes double-stranded DNA bulge and induces DNA damage. 2 Fluoro (2F) Modified EpCAM RNA aptamer is a 23-mer that targets EpCAM protein, expressed on the surface of epithelial tumor cells. Understanding the interaction between NCS and the ligand is important for carrying out the targeted tumor therapy. In this study, we have investigated the biophysical interactions between NCS and 2-fluro Modified EpCAM RNA aptamer using Circular Dichroism (CD) and Infra-Red (IR) spectroscopy. The aromatic amino acid residues spanning the β sheets of NCS are found to participate in intermolecular interactions with 2 F Modified EpCAM RNA aptamer. In-silico modeling and simulation studies corroborate with CD spectra data. Furthermore, it reinforces the involvement of C and D1 strand of NCS in intermolecular interactions with EpCAM RNA aptamer. This the first report on interactions involved in the stabilization of NCS-EpCAM aptamer complex and will aid in the development of therapeutic modalities towards targeted Cancer therapy.

Keywords

CD spectroscopy; EpCAM aptamer; Insilico modeling; Neocarzinostatin.

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