2. Academic Validation
  3. Oenothein B inhibits human non-small cell lung cancer A549 cell proliferation by ROS-mediated PI3K/Akt/NF-κB signaling pathway

Oenothein B inhibits human non-small cell lung cancer A549 cell proliferation by ROS-mediated PI3K/Akt/NF-κB signaling pathway

  • Chem Biol Interact. 2019 Jan 25;298:112-120. doi: 10.1016/j.cbi.2018.09.021.
Xiaodong Pei 1,  Junsong Xiao 2,  Guijiang Wei 3,  Yumeng Zhang 4,  Feng Lin 1,  Zhaocheng Xiong 5,  Lan Lu 6,  Xueli Wang 7,  Guangfu Pang 8,  Yan Jiang 8,  Lihe Jiang 9
Affiliations

Affiliations

  • 1 College of Light Industry and Food Engineering, Guangxi University, Nanning, 530004, PR China; Medical College, Guangxi University, Nanning, Guangxi, 530004, PR China.
  • 2 Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing Technology and Business University-BTBU, Beijing, 100048, PR China.
  • 3 Medical College, Guangxi University, Nanning, Guangxi, 530004, PR China.
  • 4 Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, FL, 33620, USA.
  • 5 State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, 530004, PR China.
  • 6 Sichuan Industrial Institute of Antibiotics, Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province Chengdu University, 610106, Chengdu, PR China.
  • 7 Guangxi Key Laboratory for Agro-Environment and Agro-Product Safety, Agriculture College, Guangxi University, PR China.
  • 8 School of Laboratory Medicine, YouJiang Medical University for Nationaties, No. 98 Chengxiang Road, Baise, Guangxi, 533000, PR China.
  • 9 College of Light Industry and Food Engineering, Guangxi University, Nanning, 530004, PR China; Medical College, Guangxi University, Nanning, Guangxi, 530004, PR China. Electronic address: [email protected].
PMID: 30452899 DOI: 10.1016/j.cbi.2018.09.021
Abstract

Oenothein B has a wide range of biological activities. The present study probed into the underlying mechanism on how Oenothein B inhibits the proliferation of a lung Cancer line A549. Our results showed that Oenothein B effectively inhibited the proliferation of A549 cells by inducing Apoptosis and arresting cells at G1 stage. Furthermore, Oenothein B not only increased the level of intracellular Reactive Oxygen Species (ROS), but also induced the upregulation of intracellular apoptotic triggers (cleavage Caspase-3, PARP, cytochrome c level in the cytosol, Bax). Moreover, ROS inhibitor (N-acetyl-L-cystein, NAC) and PI3K agonist (Insulin-like growth factor 1, IGF-1) could resist cell proliferation inhibition induced by Oenothein B, respectively. ROS inhibitor significantly abrogated the activation of Caspase 3/7 and 9 in the presence of Oenothein B. Additionally, suppression of p-PI3K and p-Akt, p-NF-κB by Oenothein B could be compensated by treatment with ROS inhibitor. To summarize, these results demonstrated that Oenothein B was able to prevent cell proliferation probably via ROS-mediated PI3K/Akt/NF-κB signaling pathway.

Keywords

A549 cell; NF-kB; Oenothein B; PI3K/Akt; ROS.

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