2. Academic Validation
  3. Anti-Inflammatory Effects of Aurantio-Obtusin from Seed of Cassia obtusifolia L. through Modulation of the NF-κB Pathway

Anti-Inflammatory Effects of Aurantio-Obtusin from Seed of Cassia obtusifolia L. through Modulation of the NF-κB Pathway

  • Molecules. 2018 Nov 27;23(12):3093. doi: 10.3390/molecules23123093.
Jingyi Hou 1 2,  Yu Gu 3 4,  Shuai Zhao 5 6,  Mengqi Huo 7 8,  Shifeng Wang 9 10,  Yanling Zhang 11 12,  Yanjiang Qiao 13 14,  Xi Li 15 16
Affiliations

Affiliations

  • 1 Research Center of Traditional Chinese Medicine Information Engineering, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. [email protected].
  • 2 Beijing Key Laboratory of Traditional Chinese Medicine Basics and New Drug Research, Research Center of Traditional Chinese Medicine Information Engineering, Beijing University of Chinese Medicine, Beijing 100102, China. [email protected].
  • 3 Research Center of Traditional Chinese Medicine Information Engineering, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. [email protected].
  • 4 Beijing Key Laboratory of Traditional Chinese Medicine Basics and New Drug Research, Research Center of Traditional Chinese Medicine Information Engineering, Beijing University of Chinese Medicine, Beijing 100102, China. [email protected].
  • 5 Research Center of Traditional Chinese Medicine Information Engineering, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. [email protected].
  • 6 Beijing Key Laboratory of Traditional Chinese Medicine Basics and New Drug Research, Research Center of Traditional Chinese Medicine Information Engineering, Beijing University of Chinese Medicine, Beijing 100102, China. [email protected].
  • 7 Research Center of Traditional Chinese Medicine Information Engineering, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. [email protected].
  • 8 Beijing Key Laboratory of Traditional Chinese Medicine Basics and New Drug Research, Research Center of Traditional Chinese Medicine Information Engineering, Beijing University of Chinese Medicine, Beijing 100102, China. [email protected].
  • 9 Research Center of Traditional Chinese Medicine Information Engineering, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. [email protected].
  • 10 Beijing Key Laboratory of Traditional Chinese Medicine Basics and New Drug Research, Research Center of Traditional Chinese Medicine Information Engineering, Beijing University of Chinese Medicine, Beijing 100102, China. [email protected].
  • 11 Research Center of Traditional Chinese Medicine Information Engineering, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. [email protected].
  • 12 Beijing Key Laboratory of Traditional Chinese Medicine Basics and New Drug Research, Research Center of Traditional Chinese Medicine Information Engineering, Beijing University of Chinese Medicine, Beijing 100102, China. [email protected].
  • 13 Research Center of Traditional Chinese Medicine Information Engineering, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. [email protected].
  • 14 Beijing Key Laboratory of Traditional Chinese Medicine Basics and New Drug Research, Research Center of Traditional Chinese Medicine Information Engineering, Beijing University of Chinese Medicine, Beijing 100102, China. [email protected].
  • 15 Research Center of Traditional Chinese Medicine Information Engineering, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. [email protected].
  • 16 Beijing Key Laboratory of Traditional Chinese Medicine Basics and New Drug Research, Research Center of Traditional Chinese Medicine Information Engineering, Beijing University of Chinese Medicine, Beijing 100102, China. [email protected].
PMID: 30486383 DOI: 10.3390/molecules23123093
Abstract

Aurantio-obtusin, an anthraquinone compound, isolated from dried seeds of Cassia obtusifolia L. (syn. Senna obtusifolia; Fabaceae) and Cassia tora L. (syn. Senna tora). Although the biological activities of Semen Cassiae have been reported, the anti-inflammatory mechanism of aurantio-obtusin, its main compound, on RAW264.7 cells, remained unknown. We investigated the anti-inflammatory effect of aurantio-obtusin on lipopolysaccharide- (LPS)-induced RAW264.7 cells in vitro and elucidated the possible underlying molecular mechanisms. Nitric oxide production (NO) and prostaglandin E₂ (PGE₂) were measured by the Griess colorimetric method and enzyme-linked immunosorbent assay (ELISA), respectively. Protein expression levels of cyclooxygenase 2 (COX-2) were monitored by cell-based ELISA. Interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-α) synthesis were analyzed using ELISA. The mRNA expression of nitric oxide synthase (iNOS), COX-2, and the critical pro-inflammatory cytokines (IL-6 and TNF-α) were detected by quantitative Real-Time PCR. Aurantio-obtusin significantly decreased the production of NO, PGE₂, and inhibited the protein expression of COX-2, TNF-α and IL-6, which were similar to those gene expression of iNOS, COX-2, TNF-α and IL-6 (p < 0.01). Consistent with the pro-inflammatory gene expression, the Aurantio-obtusin efficiently reduced the LPS-induced activation of nuclear factor-κB in RAW264.7 cells. These results suggested that aurantio-obtusin may function as a therapeutic agent and can be considered in the further development of treatments for a variety of inflammatory diseases. Further studies may provide scientific evidence for the use of aurantio-obstusin as a new therapeutic agent for inflammation-related diseases.

Keywords

NF-κB; Semen Cassiae; anthraquinone; inflammation.

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