2. Academic Validation
  3. Paris saponin VII reverses chemoresistance in breast MCF-7/ADR cells

Paris saponin VII reverses chemoresistance in breast MCF-7/ADR cells

  • J Ethnopharmacol. 2019 Mar 25;232:47-54. doi: 10.1016/j.jep.2018.12.018.
Yuhua Li 1,  Yang Sun 2,  Tianle Tang 3,  Yinbo Niu 4,  Xiaoqiang Li 5,  Ming Xie 6,  Hongchuan Jin 7,  Qibing Mei 8
Affiliations

Affiliations

  • 1 School of Medicine, Zhejiang University, Hangzhou 310020, Zhejiang, PR China; Laboratory of Oncological Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou 646000, Sichuan, PR China. Electronic address: [email protected].
  • 2 Key Laboratory of Gastrointestinal Pharmacology of Chinese Materia Medica of the State Administration of Traditional Chinese Medicine, Department of Pharmacology, School of Pharmacy, The Fourth Military Medical University, Xi'an 710032, Shaanxi, PR China. Electronic address: [email protected].
  • 3 Key Laboratory of Gastrointestinal Pharmacology of Chinese Materia Medica of the State Administration of Traditional Chinese Medicine, Department of Pharmacology, School of Pharmacy, The Fourth Military Medical University, Xi'an 710032, Shaanxi, PR China. Electronic address: [email protected].
  • 4 Faculty of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, PR China. Electronic address: [email protected].
  • 5 Key Laboratory of Gastrointestinal Pharmacology of Chinese Materia Medica of the State Administration of Traditional Chinese Medicine, Department of Pharmacology, School of Pharmacy, The Fourth Military Medical University, Xi'an 710032, Shaanxi, PR China. Electronic address: [email protected].
  • 6 Department of Pathology, No. 422 Hospital of PLA, Zhanjiang 524005, Guangdong, PR China. Electronic address: [email protected].
  • 7 School of Medicine, Zhejiang University, Hangzhou 310020, Zhejiang, PR China. Electronic address: [email protected].
  • 8 Laboratory of Oncological Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou 646000, Sichuan, PR China; Key Laboratory of Gastrointestinal Pharmacology of Chinese Materia Medica of the State Administration of Traditional Chinese Medicine, Department of Pharmacology, School of Pharmacy, The Fourth Military Medical University, Xi'an 710032, Shaanxi, PR China. Electronic address: [email protected].
PMID: 30552993 DOI: 10.1016/j.jep.2018.12.018
Abstract

Ethnopharmacological relevance: The development of a multidrug-resistant (MDR) phenotype is a main obstacle to the successful treatment of breast Cancer. Saponins of several herbs are considered as promising candidates for drug resistance treatment. We extracted Paris saponin VII (PS VII) from Trillium tschonoskii Maxim. and investigated whether it could sensitize chemoresistant breast Cancer cells MCF-7/ADR to the cytotoxic effects of adriamycin.

Materials and methods: MCF-7/ADR cells were exposed to 0.5 μM PSVII plus different concentrations of adriamycin (0-100 μM). Then, MTT assay and adriamycin accumulation assay were used to assess cell proliferation and intracellular adriamycin retention. P glycoprotein levels and intracellular rhodamine 123 (Rh-123) accumulations were investigated to measure the expression and activity of P-glycoprotein. A xenograft model of nude mouse was utilized to observe the effect of PSVII in vivo.

Results: Treatment with PSVII influenced cell viability of MCF-7/ADR cells, as well as sensitized MCF-7/ADR cells to the cytotoxic effects of adriamycin. Moreover, PSVII significantly downregulated MDR1 expression in MCF-7/ADR cells. Intravenous administration of PSVII significantly enhanced Anticancer efficacy of adriamycin to MCF-7/ADR xenograft model in nude mice.

Conclusion: These findings suggested a possible application of PSVII in combination with chemotherapy and/or as neo-adjuvant therapy in the treatment of MDR breast Cancer.

Keywords

Multidrug resistance (MDR); P-glycoprotein (P-gp); Paris saponin VII (PSVII).

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