2. Academic Validation
  3. Genomics-driven discovery of a new cyclodepsipeptide from the guanophilic fungus Amphichorda guana

Genomics-driven discovery of a new cyclodepsipeptide from the guanophilic fungus Amphichorda guana

  • Org Biomol Chem. 2021 Mar 11;19(9):1960-1964. doi: 10.1039/d1ob00100k.
Min Liang 1,  Hai-Ning Lyu 2,  Zi-Ying Ma 1,  Er-Wei Li 3,  Lei Cai 3,  Wen-Bing Yin 1
Affiliations

Affiliations

  • 1 State Key Laboratory of Mycology and CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P.R. China. [email protected] [email protected] and University of Chinese Academy of Sciences, Beijing, 100049, China.
  • 2 State Key Laboratory of Mycology and CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P.R. China. [email protected] [email protected] and Artemisinin Research Center, and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
  • 3 State Key Laboratory of Mycology and CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P.R. China. [email protected] [email protected].
PMID: 33599675 DOI: 10.1039/d1ob00100k
Abstract

Two potential non-ribosomal peptide synthetases (NRPSs) were identified in the genome of a guanophilic fungus Amphichorda guana by bioinformatics analysis and gene knockout experiments. Liquid chromatography coupled with mass spectrometry (LC-MS) guided isolation led to the discovery of a new cyclodepsipeptide isaridin H (1) and seven known analogs, desmethylisaridin E (2), isaridin E (3), isariin A (4), iso-isariin B (5), iso-isariin D (6), isariin E (7), and nodupetide (8). The absolute configuration of isaridin H (1) was achieved by Marfey's method. Isaridin H (1) showed significant Antifungal activity against Botrytis cinerea and Alternaria solani.

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