1. Academic Validation
  2. Mammalian Arginase Inhibitory Activity of Methanolic Extracts and Isolated Compounds from Cyperus Species

Mammalian Arginase Inhibitory Activity of Methanolic Extracts and Isolated Compounds from Cyperus Species

  • Molecules. 2021 Mar 18;26(6):1694. doi: 10.3390/molecules26061694.
Kamel Arraki 1 Perle Totoson 1 Alain Decendit 2 Andy Zedet 1 Justine Maroilley 1 Alain Badoc 2 Céline Demougeot 1 Corine Girard 1
Affiliations

Affiliations

  • 1 PEPITE EA 4267, FHU INCREASE, University Bourgogne Franche-Comté, 25000 Besançon, France.
  • 2 MIB-UR Oenologie, EA 4577, USC 1366 INRA, University of Bordeaux, ISVV, 33882 Villenave d'Ornon, France.
Abstract

Polyphenolic enriched extracts from two species of Cyperus, Cyperus glomeratus and Cyperus thunbergii, possess mammalian Arginase inhibitory capacities, with the percentage inhibition ranging from 80% to 95% at 100 µg/mL and 40% to 64% at 10 µg/mL. Phytochemical investigation of these species led to the isolation and identification of two new natural stilbene oligomers named thunbergin A-B (1-2), together with three other Stilbenes, trans-resveratrol (3), trans-scirpusin A (4), trans-cyperusphenol A (6), and two Flavonoids, aureusidin (5) and luteolin (7), which were isolated for the first time from C.thunbergii and C. glomeratus. Structures were established on the basis of the spectroscopic data from MS and NMR experiments. The Arginase inhibitory activity of compounds 1-7 was evaluated through an in vitro Arginase inhibitory assay using purified liver bovine Arginase. As a result, five compounds (1, 4-7) showed significant inhibition of Arginase, with IC50 values between 17.6 and 60.6 µM, in the range of those of the natural Arginase Inhibitor piceatannol (12.6 µM). In addition, methanolic extract from Cyperus thunbergii exhibited an endothelium and NO-dependent vasorelaxant effect on thoracic aortic rings from rats and improved endothelial dysfunction in an adjuvant-induced arthritis rat model.

Keywords

Cyperus glomeratus; Cyperus thunbergii; arginase inhibitors; flavonoids; stilbenes; vasorelaxante activity.

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