2. Academic Validation
  3. Induction of Fungal Secondary Metabolites by Co-Culture with Actinomycete Producing HDAC Inhibitor Trichostatins

Induction of Fungal Secondary Metabolites by Co-Culture with Actinomycete Producing HDAC Inhibitor Trichostatins

  • J Microbiol Biotechnol. 2023 Nov 28;33(11):1437-1447. doi: 10.4014/jmb.2301.01017.
Gwi Ja Hwang 1,  Jongtae Roh 1 2,  Sangkeun Son 3,  Byeongsan Lee 1,  Jun-Pil Jang 1,  Jae-Seoun Hur 4,  Young-Soo Hong 1 2,  Jong Seog Ahn 1 2,  Sung-Kyun Ko 1 2,  Jae-Hyuk Jang 1 2
Affiliations

Affiliations

  • 1 Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Republic of Korea.
  • 2 Department of Biomolecular Science, KRIBB school of Bioscience, University of Science and Technology (UST), Daejeon 34141, Republic of Korea.
  • 3 Antimicrobial Discovery Center, Department of Biology, Northeastern University, Boston 02115 MA, USA.
  • 4 Korean Lichen Research Institute, Sunchon National University, Suncheon 57922, Republic of Korea.
PMID: 37670557 DOI: 10.4014/jmb.2301.01017
Abstract

A recently bioinformatic analysis of genomic sequences of fungi indicated that fungi are able to produce more secondary metabolites than expected. Despite their potency, many biosynthetic pathways are silent in the absence of specific culture conditions or chemical cues. To access cryptic metabolism, 108 Fungal strains isolated from various sites were cultured with or without Streptomyces sp. 13F051 which mainly produces trichostatin analogues, followed by comparison of metabolic profiles using LC-MS. Among the 108 Fungal strains, 14 produced secondary metabolites that were not recognized or were scarcely produced in mono-cultivation. Of these two Fungal strains, Myrmecridium schulzeri 15F098 and Scleroconidioma sphagnicola 15S058 produced four new compounds (1-4) along with a known compound (5), demonstrating that all four compounds were produced by physical interaction with Streptomyces sp. 13F051. Bioactivity evaluation indicated that compounds 3-5 impede migration of MDA-MB-231 breast Cancer cells.

Keywords

Co-culture system; fungal cryptic secondary metabolites; physical interaction; structure determination.

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