2. Academic Validation
  3. Lipids Extracted from Mycobacterial Membrane and Enveloped PLGA Nanoparticles for Encapsulating Antibacterial Drugs Elicit Synergistic Antimicrobial Response against Mycobacteria

Lipids Extracted from Mycobacterial Membrane and Enveloped PLGA Nanoparticles for Encapsulating Antibacterial Drugs Elicit Synergistic Antimicrobial Response against Mycobacteria

  • Mol Pharm. 2024 May 6;21(5):2238-2249. doi: 10.1021/acs.molpharmaceut.3c01001.
Xueyu Pu 1 2 3,  Yuanyuan Wang 1 2 3,  Xi Wang 1 2 3,  Xiaoqing Sang 1 2 3,  Miaomiao Jiang 2,  DaWei Qi 4,  Xin Zhao 5 6,  Rong Chen 5 6,  Jianwei Li 4,  Xiang Liu 5 6,  Zhidong Liu 2,  Jian Yang 1 2 3
Affiliations

Affiliations

  • 1 Tianjin Key Laboratory of Chinese Medicine Pharmacology. Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
  • 2 State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
  • 3 Haihe Laboratory of Modern Chinese Medicine, Tianjin 301617, China.
  • 4 Medcity Research Laboratory, University of Turku, Tykistokatu 6, FI-20520 Turku, Finland.
  • 5 State Key Laboratory of Medicinal Chemical Biology, Frontiers Science Center for Cell Responses, College of Life Sciences, Nankai University, Tianjin 300071, China.
  • 6 Infectious Disease Drug Discovery Institute, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China.
PMID: 38622497 DOI: 10.1021/acs.molpharmaceut.3c01001
Abstract

Tuberculosis (TB) is a chronic disease caused byMycobacterium tuberculosis (Mtb), which shows a long treatment cycle often leads to drug resistance, making treatment more difficult. Immunogens present in the pathogen's cell membrane can stimulate endogenous immune responses. Therefore, an effective lipid-based vaccine or drug delivery vehicle formulated from the pathogen's cell membrane can improve treatment outcomes. Herein, we extracted and characterized lipids fromMycobacterium smegmatis, and the extracts contained lipids belonging to numerous lipid classes and compounds typically found associated with mycobacteria. The extracted lipids were used to formulate biomimetic lipid reconstituted nanoparticles (LrNs) and LrNs-coated poly(lactic-co-glycolic acid) nanoparticles (PLGA-LrNs). Physiochemical characterization and results of morphology suggested that PLGA-LrNs exhibited enhanced stability compared with LrNs. And both of these two types of nanoparticles inhibited the growth of M. smegmatis. After loading different drugs, PLGA-LrNs containing berberine or coptisine strongly and synergistically prevented the growth of M. smegmatis. Altogether, the Bacterial membrane lipids we extracted with Antibacterial activity can be used as nanocarrier coating for synergistic Antibacterial treatment of M. smegmatis─an alternative model of Mtb, which is expected as a novel therapeutic system for TB treatment.

Keywords

antibacterial; lipid extract; lipid reconstituted nanoparticles; mycobacterium membrane.

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