2. Academic Validation
  3. Camellia sinensis polysaccharide attenuates inflammatory responses via the ROS-mediated pathway by endocytosis

Camellia sinensis polysaccharide attenuates inflammatory responses via the ROS-mediated pathway by endocytosis

  • Int J Biol Macromol. 2024 May;267(Pt 2):131674. doi: 10.1016/j.ijbiomac.2024.131674.
Mingzhu Zhang 1,  Huaguang Qin 1,  Lijun Xiang 1,  Lujing An 1,  Xiaoling Zhang 1,  Kexin Li 1,  Kai Wu 1,  Xinyao Fei 1,  Wenhui Fan 1,  Xinyun Xu 1,  Pengfei Xu 1,  Yan Wu 2,  Dan Mu 3
Affiliations

Affiliations

  • 1 The Province Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui, School of Life Science, Anqing Normal University, Anqing 246011, China.
  • 2 The Province Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui, School of Life Science, Anqing Normal University, Anqing 246011, China. Electronic address: [email protected].
  • 3 The Province Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui, School of Life Science, Anqing Normal University, Anqing 246011, China. Electronic address: [email protected].
PMID: 38641285 DOI: 10.1016/j.ijbiomac.2024.131674
Abstract

Polysaccharide CSTPs extracted from Camellia sinensis tea-leaves possessed unique against oxidative damage by scavenging ROS. Herein, acid tea polysaccharide CSTPs-2 with tightly packed molecular structure was isolated, purified and characterized in this research. Furthermore, the effects of CSTPs-2 on ROS-involved inflammatory responses and its underlying mechanisms were investigated. The results suggest that CSTPs-2 dramatically reduced the inflammatory cytokines overexpression and LPS-stimulated cell damage. CSTPs-2 could trigger the dephosphorylation of downstream Akt/MAPK/NF-κB signaling proteins and inhibit nuclear transfer of p-NF-κB to regulate the synthesis and release of inflammatory mediators in LPS-stimulated cells by ROS scavenging. Importantly, the impact of CSTPs-2 in downregulating pro-inflammatory cytokines and mitigating ROS overproduction is associated with clathrin- or caveolae-mediated endocytosis uptake mechanisms, rather than TLR-4 receptor-mediated endocytosis. This study presents a novel perspective for investigating the cellular uptake mechanism of Polysaccharides in the context of anti-inflammatory mechanisms.

Keywords

AKT/MAPK/NF-κB; Anti-inflammation; Endocytosis uptake mechanism; ROS; Tea polysaccharides.

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