1. Academic Validation
  2. Isoglutaminyl Cyclase Overexpression Enhances KYSE30 Cancer Cell Proliferation and Migration via the MAPK Signaling Pathway

Isoglutaminyl Cyclase Overexpression Enhances KYSE30 Cancer Cell Proliferation and Migration via the MAPK Signaling Pathway

  • J Proteome Res. 2024 May 3;23(5):1859-1870. doi: 10.1021/acs.jproteome.4c00197.
Xiaojie Chen 1 Xi Yu 2 Yangqing Cui 1 Lang Du 1 Qingqing Zhou 2 Wei Xiong 1 Chenyang Li 1 Chenshu Xu 1 Haiqiang Wu 1
Affiliations

Affiliations

  • 1 School of Pharmacy, Shenzhen University Medical School, Shenzhen 518055, China.
  • 2 School of Biomedical Engineering, Shenzhen University Medical School, Shenzhen 518055, China.
Abstract

To understand how upregulated isoglutaminyl cyclase (isoQC) is involved in the initiation of diseases such as Cancer, we developed a human KYSE30 carcinoma cell model in which isoQC was stably overexpressed. GO and KEGG analysis of the DEGs (228) and DEPs (254) respectively implicated isoQC on the proliferation invasion and metastasis of cells and suggested that isoQC might participate in the regulation of MAPK, Ras, circadian rhythm, and related pathways. At the functional level, isoQC-overexpressing KYSE30 cells showed enhanced proliferation, migration, and invasion capacity. Next, we decided to study the precise effect of isoQC overexpression on JNK, p-JNK, Akt, p-AKT, ERK, p-ERK, and PER2, as RNA levels of these proteins are significantly correlated with signal levels indicated in RNA-Seq analysis, and these candidates are the top correlated DEPs enriched in RT-qPCR analysis. We saw that only p-ERK expression was inhibited, while PER2 was increased. These phenotypes were inhibited upon exposure to PER2 inhibitor KL044, which allowed for the restoration of p-ERK levels. These data support upregulated isoQC being able to promote Cancer cell proliferation and migration in vitro, likely by helping to regulate the MAPK and Ras signaling pathways, and the circadian protein PER2 might be a potential mediator.

Keywords

KYSE30 cells; MAPK signaling pathway; RNA-Seq; iTRAQ; isoglutaminiyl cyclase.

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