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  4. Msr-Ratio

Msr-Ratio  (Synonyms: Msr-green)

Cat. No.: HY-D1257 Purity: 99.13%
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Msr-Ratio (Msr-green) is a ratiometric fluorescent probe of methionine sulfoxide reductase (λex=375 nm, λem=550 nm). Msr-Ratio is used for monitoring the enzyme activity in vitro and in live cells.

For research use only. We do not sell to patients.

Msr-Ratio Chemical Structure

Msr-Ratio Chemical Structure

CAS No. : 2290635-22-0

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1 mg USD 242 In-stock
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Based on 1 publication(s) in Google Scholar

Msr-Ratio Related Antibodies

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Description

Msr-Ratio (Msr-green) is a ratiometric fluorescent probe of methionine sulfoxide reductase (λex=375 nm, λem=550 nm). Msr-Ratio is used for monitoring the enzyme activity in vitro and in live cells[1].

In Vitro

Msr-Ratio is converted to its corresponding sulfide form by methionine sulfoxide reductase. Msr-Ratio displays favorable properties such as a nearly 400-fold fluorescence change, fast response rate (<30 min), large Stokes shift (120 nm), and green emission (550 nm)[1].
There is a very weak background signal in live HL60 cells after the addition of Msr-Ratio. As the Msr-Ratio (10 μM) incubation time increased in cells from 0 to 8 h, the fluorescence signal constantly strengthened[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Msr-Ratio Related Antibodies
Molecular Weight

310.37

Formula

C18H14O3S
CAS No.
Appearance

Solid

Color

White to off-white

Emission (Em)

550
Excitation (Ex)

375
SMILES

CS(C1=CC=C(/C=C/C(OC2=C3C=CC=C2)=CC3=O)C=C1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (322.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2220 mL 16.1098 mL 32.2196 mL
5 mM 0.6444 mL 3.2220 mL 6.4439 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

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=
Concentration (final)

C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2220 mL 16.1098 mL 32.2196 mL 80.5490 mL
5 mM 0.6444 mL 3.2220 mL 6.4439 mL 16.1098 mL
10 mM 0.3222 mL 1.6110 mL 3.2220 mL 8.0549 mL
15 mM 0.2148 mL 1.0740 mL 2.1480 mL 5.3699 mL
20 mM 0.1611 mL 0.8055 mL 1.6110 mL 4.0275 mL
25 mM 0.1289 mL 0.6444 mL 1.2888 mL 3.2220 mL
30 mM 0.1074 mL 0.5370 mL 1.0740 mL 2.6850 mL
40 mM 0.0805 mL 0.4027 mL 0.8055 mL 2.0137 mL
50 mM 0.0644 mL 0.3222 mL 0.6444 mL 1.6110 mL
60 mM 0.0537 mL 0.2685 mL 0.5370 mL 1.3425 mL
80 mM 0.0403 mL 0.2014 mL 0.4027 mL 1.0069 mL
100 mM 0.0322 mL 0.1611 mL 0.3222 mL 0.8055 mL
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Msr-Ratio Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Msr-Ratio2290635-22-0Msr-greenFluorescent DyeratiometricfluorescentmethioninesulfoxidereductaselivecellsInhibitorinhibitorinhibit

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