2. PI3K/Akt/mTOR Apoptosis Cytoskeleton Cell Cycle/DNA Damage
  3. Akt Apoptosis mTOR PI3K Microtubule/Tubulin
  4. PI3K/Akt/mTOR-IN-4

PI3K/Akt/mTOR-IN-4 (compound 4r) is a potent PI3K/Akt/mTOR and tubulin polymerization inhibitor. PI3K/Akt/mTOR-IN-4 induce apoptosis and cell cycle arrest at G2/M phase. PI3K/Akt/mTOR-IN-4 decreases the expression of p-PI3K, p-Akt, and p-mTOR, β-tubulin.

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PI3K/Akt/mTOR-IN-4 Chemical Structure

PI3K/Akt/mTOR-IN-4 Chemical Structure

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PI3K/Akt/mTOR-IN-4 Related Antibodies

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Description

PI3K/Akt/mTOR-IN-4 (compound 4r) is a potent PI3K/Akt/mTOR and tubulin polymerization inhibitor. PI3K/Akt/mTOR-IN-4 induce apoptosis and cell cycle arrest at G2/M phase. PI3K/Akt/mTOR-IN-4 decreases the expression of p-PI3K, p-Akt, and p-mTOR, β-tubulin[1].

In Vitro

PI3K/Akt/mTOR-IN-4 (compound 4r) (0-100 µM; 48 h) shows antiproliferative activity with IC50s of 3.38, 5.03, 7.24, 21.08, 23.96 µM for SiHa, HeLa, Ca Ski, LO2, HEK-293t cells, respectively[1].
PI3K/Akt/mTOR-IN-4 (0-16 µM; 24 h) induces apoptosis and cell cycle arrest at G2/M phase[1].
PI3K/Akt/mTOR-IN-4 (4, 8,16 µM; 24 h) decreases the expression of phosphorylation of PI3K, Akt, mTOR level and β-tubulin protein in a dose-dependent manner[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PI3K/Akt/mTOR-IN-4 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: SiHa, HeLa, Ca Ski, LO2, HEK-293t cells
Concentration: 0-100 µM
Incubation Time: 48 h
Result: Inhibited cell proliferative with IC50s of 3.38, 5.03, 7.24, 21.08, 23.96 µM for SiHa, HeLa, Ca Ski, LO2, HEK-293t cells, respectively.

Cell Cycle Analysis[1]

Cell Line: SiHa cells
Concentration: 0-16 µM
Incubation Time: 24 h
Result: Induced cell cycle arrest at G2/M phase with G2/M phase cells accumulating from 5.24 % (Ctrl) to 28.37 % (16 μM).

Apoptosis Analysis[1]

Cell Line: SiHa cells
Concentration: 0-16 µM
Incubation Time: 24 h
Result: Induced apoptosis the percentage of apoptotic cells were increased from 11.62 % (Ctrl) to 98.56 % (16 μM).

Western Blot Analysis[1]

Cell Line: SiHa cells
Concentration: 4, 8, 16 μM
Incubation Time: 24 h
Result: Decreased the expression of phosphorylation of PI3K, Akt, and mTOR, β-tubulin in a dose-dependent manner.
In Vivo

PI3K/Akt/mTOR-IN-4 (0-400 μM; 0-96 h) shows no toxicity for zebrafish embryos[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: zebrafish embryos[1]
Dosage: 0, 12.5, 25, 50, 100, 200, 400 μM
Administration: 0-96 h
Result: Showed no toxicity for zebrafish embryos.
Molecular Weight

500.56

Formula

C29H29FN4O3
SMILES

COC1=C(OC)C(OC)=CC(C2=NC3=CC(N4CCN(C)CC4)=C(C#CC5=CC=CC=C5F)C=C3N2)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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PI3K/Akt/mTOR-IN-4 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PI3K/Akt/mTOR-IN-4AktApoptosismTORPI3KMicrotubule/TubulinPKBProtein kinase BMammalian target of RapamycinPhosphoinositide 3-kinasetubulin polymerizationapoptosisG2/Mp-PI3Kp-Aktand p-mTORzebrafish embryosInhibitorinhibitorinhibit

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