1. Peptides
  2. Pyr-Arg-Thr-Lys-Arg-AMC TFA

Pyr-Arg-Thr-Lys-Arg-AMC TFA is a AMC peptide. AMC is a decapeptide that is specifically hydrolyzed by proteases such as trypsin and thrombin. The AMC peptide can be used to determine the activity of protease and the potency of enzyme inhibitors.

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Pyr-Arg-Thr-Lys-Arg-AMC TFA Chemical Structure

Pyr-Arg-Thr-Lys-Arg-AMC TFA Chemical Structure

CAS No. : 1255501-99-5

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5 mg USD 110 In-stock
10 mg USD 180 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

Pyr-Arg-Thr-Lys-Arg-AMC TFA is a AMC peptide. AMC is a decapeptide that is specifically hydrolyzed by proteases such as trypsin and thrombin. The AMC peptide can be used to determine the activity of protease and the potency of enzyme inhibitors[1].

Molecular Weight

941.95

Formula

C39H58F3N13O11

CAS No.
Appearance

Solid

Color

White to off-white

Sequence

{Pyr}-Arg-Thr-Lys-Arg-{AMC}

Sequence Shortening

{Pyr}-RTKR-{AMC}

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (106.16 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0616 mL 5.3081 mL 10.6163 mL
5 mM 0.2123 mL 1.0616 mL 2.1233 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.0616 mL 5.3081 mL 10.6163 mL 26.5407 mL
5 mM 0.2123 mL 1.0616 mL 2.1233 mL 5.3081 mL
10 mM 0.1062 mL 0.5308 mL 1.0616 mL 2.6541 mL
15 mM 0.0708 mL 0.3539 mL 0.7078 mL 1.7694 mL
20 mM 0.0531 mL 0.2654 mL 0.5308 mL 1.3270 mL
25 mM 0.0425 mL 0.2123 mL 0.4247 mL 1.0616 mL
30 mM 0.0354 mL 0.1769 mL 0.3539 mL 0.8847 mL
40 mM 0.0265 mL 0.1327 mL 0.2654 mL 0.6635 mL
50 mM 0.0212 mL 0.1062 mL 0.2123 mL 0.5308 mL
60 mM 0.0177 mL 0.0885 mL 0.1769 mL 0.4423 mL
80 mM 0.0133 mL 0.0664 mL 0.1327 mL 0.3318 mL
100 mM 0.0106 mL 0.0531 mL 0.1062 mL 0.2654 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Pyr-Arg-Thr-Lys-Arg-AMC TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Pyr-Arg-Thr-Lys-Arg-AMC TFA
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