RBC6

Cat. No.: HY-125987 Purity: 98.88%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

RBC6 is an inhibitor of GTPases RalA. RBC6 inhibits binding of Ral to its effector RALBP1. RBC6 also inhibits Ral-mediated cell spreading of murine embryonic fibroblasts, as well as anchorage-independent growth of human cancer celllines.

For research use only. We do not sell to patients.

RBC6 Chemical Structure

CAS No. : 381186-64-7

Size	Price	Stock	Quantity
5 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 260	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 520	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 830	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1320	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Purity & Documentation
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Description

Molecular Weight

365.21

Formula

C₁₆H₁₄Cl₂N₄O₂

CAS No.

381186-64-7

Appearance

Solid

Color

Off-white to light yellow

SMILES

N#CC(C1C2=CC(Cl)=CC(Cl)=C2O)=C(N)OC3=C1C(CCC)=NN3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (273.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7382 mL	13.6908 mL	27.3815 mL
5 mM	0.5476 mL	2.7382 mL	5.4763 mL
10 mM	0.2738 mL	1.3691 mL	2.7382 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.88%

Select Batch:

Data Sheet (280 KB) SDS (0 KB)

COA (261 KB) HNMR (277 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Yan C, et al. Discovery and characterization of small molecules that target the GTPase Ral. Nature. 2014 Nov 20;515(7527):443-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7382 mL	13.6908 mL	27.3815 mL	68.4538 mL
	5 mM	0.5476 mL	2.7382 mL	5.4763 mL	13.6908 mL
	10 mM	0.2738 mL	1.3691 mL	2.7382 mL	6.8454 mL
	15 mM	0.1825 mL	0.9127 mL	1.8254 mL	4.5636 mL
	20 mM	0.1369 mL	0.6845 mL	1.3691 mL	3.4227 mL
	25 mM	0.1095 mL	0.5476 mL	1.0953 mL	2.7382 mL
	30 mM	0.0913 mL	0.4564 mL	0.9127 mL	2.2818 mL
	40 mM	0.0685 mL	0.3423 mL	0.6845 mL	1.7113 mL
	50 mM	0.0548 mL	0.2738 mL	0.5476 mL	1.3691 mL
	60 mM	0.0456 mL	0.2282 mL	0.4564 mL	1.1409 mL
	80 mM	0.0342 mL	0.1711 mL	0.3423 mL	0.8557 mL
	100 mM	0.0274 mL	0.1369 mL	0.2738 mL	0.6845 mL

RBC6 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RBC6381186-64-7RBC 6RBC-6OthersGTPaseRalARALBP1embryonic fibroblastcancerInhibitorinhibitorinhibit

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