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AQPZ Protein, E.coli (Cell-Free, His)

Cat. No.: HY-P72013
Handling Instructions

AQPZ serves as a channel facilitating osmotically driven water movement bidirectionally. It plays a crucial role in osmoregulation, actively contributing to the maintenance of cell turgor, particularly during rapid volume expansion in actively growing cells. This channel enables the swift entry or exit of water, responding promptly to sudden changes in osmolarity, highlighting its significance in cellular water homeostasis. AQPZ Protein, E.coli (Cell-Free, His) is the recombinant E. coli-derived AQPZ protein, expressed by E. coli Cell-free , with N-6*His labeled tag. The total length of AQPZ Protein, E.coli (Cell-Free, His) is 231 a.a., with molecular weight of ~27.7 kDa.

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Description

AQPZ serves as a channel facilitating osmotically driven water movement bidirectionally. It plays a crucial role in osmoregulation, actively contributing to the maintenance of cell turgor, particularly during rapid volume expansion in actively growing cells. This channel enables the swift entry or exit of water, responding promptly to sudden changes in osmolarity, highlighting its significance in cellular water homeostasis. AQPZ Protein, E.coli (Cell-Free, His) is the recombinant E. coli-derived AQPZ protein, expressed by E. coli Cell-free , with N-6*His labeled tag. The total length of AQPZ Protein, E.coli (Cell-Free, His) is 231 a.a., with molecular weight of ~27.7 kDa.

Background

AQPZ (Aquaporin Z) protein functions as a channel that facilitates osmotically driven movement of water bidirectionally. This aquaporin plays a crucial role in osmoregulation and is involved in maintaining cell turgor during the volume expansion observed in rapidly growing cells. AQPZ enables the rapid entry or exit of water, responding swiftly to abrupt changes in osmolarity. The protein's ability to mediate water transport in both directions highlights its significance in regulating cellular hydration and ensuring proper cell volume under varying osmotic conditions.

Species

E.coli

Source

E. coli Cell-free

Tag

N-6*His

Accession

P60844 (M1-D231)

Gene ID

58388674  [NCBI]

Molecular Construction
N-term
6*His
AQPZ (M1-D231)
Accession # P60844
C-term
Synonyms
Bacterial nodulin-like intrinsic protein
AA Sequence

MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFGLTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD

Molecular Weight

Approximately 27.7 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

AQPZ Protein, E.coli (Cell-Free, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
AQPZ Protein, E.coli (Cell-Free, His)
Cat. No.:
HY-P72013
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