Cadherin-13 Protein, Rat (HEK293)

Cadherin-13 Protein is an atypical member of the cadherin family, devoid of a transmembrane domain and anchored to the exterior surface of the plasma membrane via a glycosylphosphatidylinositol anchor. And it exhibits a preference for homophilic interactions, fostering connections between neighboring cells and potentially playing a role in cell type sorting. Cadherin-13 may act as negative regulators of neural cell growth. Moreover, it may act rather as a signalling receptor participating in recognition of the environment and regulation of cell motility, proliferation and phenotype. Cadherin-13 Protein, Rat (HEK293) is the recombinant rat-derived Cadherin-13 protein, expressed by HEK293 , with tag free. The total length of Cadherin-13 Protein, Rat (HEK293) is 671 a.a., with molecular weight of ~95 kDa.

Cadherin-13 is an atypical member of the cadherin family, devoid of a transmembrane domain and anchored to the exterior surface of the plasma membrane via a glycosylphosphatidylinositol anchor. And it exhibits a preference for homophilic interactions, fostering connections between neighboring cells and potentially playing a role in cell type sorting. Cadherin-13 may act as negative regulators of neural cell growth. Cadherin-13 is highly expressed in nervous and cardiovascular system (in endothelial and smooth muscle cells). Thereby, it may facilitate progression of proliferative vascular disorders such as atherosclerosis, restenosis and tumor angiogenesis. Moreover, it may act rather as a signalling receptor participating in recognition of the environment and regulation of cell motility, proliferation and phenotype. Cadherin-13-dependent activation of PI3K/Akt/GSK3β signalling axis protects endothelial cells from oxidative stress-induced apoptosis.

Measured by the ability of the immobilized protein to inhibit the adhesion of HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 4.702 μg/mL, corresponding to a specific activity is 212.67 units/mg.

Rat

HEK293

Tag Free

Q8R490 (A22-A692)

192248  [NCBI]

AGDLDCTPGFQQKVLHIHQPAEFIEDQPVLNLTFSDCKGNKKLHYEVSSPYFKVNSDGTLVAVRNITAVGRTLFVHARTPHAEDMAELVIVGGKDIQGSLQDIFKFARTSPVPRQKRSIVVSPILIPENQRQPFPRDVGKVVDSDRPEGSKFRLTGKGVDQDPKGTFRINENTGSVSVTRTLDRETIATYQLFVETTDASGRTLEGPVPLEVIVIDQNDNRPIFREGPYIGHVMEGSPTGTTVMRMTAFDADDPATDNALLRYNIRQQTPDKPSPNMFYIDPEKGDIVTVVSPALLDRETLENPKYELIIEAQDMAGLDVGLTGTATATIVIDDKNDHSPKFTKKEFQATVEEGAVGVIVNLTVEDKDDPATGAWRAAYTIINGNPGQSFEIHTNPQTNEGMLSVVKPLDYEISAFHTLLIKVENEDPLVPDVSYGPSSTATVHITVLDVNEGPVFYPDPMMVTKQENISVGSVLLTVNATDPDSLQHQTIRYSVYKDPAGWLSINPINGTVDTTAVLDRESPFVHNSVYTALFLAIDSGNPPATGTGTLMITLEDVNDNAPVIYPTVAEVCDDARNLSVVILGASDKDLHPNTDPFKFEIHKQTVPDKVWKISKINNTHALVSLLQNLNKANYNLPIMVTDSGKPPMTNITDLRVQVCSCKNSKVDCNAA

Approximately 95 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.