CD64 Protein, Human (Biotinylated, 272a.a, HEK293, His-Avi)

CD64 protein, a high affinity receptor, mediates IgG effector functions, triggering antibody-dependent cellular cytotoxicity (ADCC). It interacts with IGHG1 and forms a signaling complex with FCERG1, contributing to immune responses. CD64 also interacts with FLNA, EPB41L2, LAT, PPL, HCK, and LYN to prevent degradation of FCGR1A and regulate immune function. CD64 Protein, Human (Biotinylated, 272a.a, HEK293, His-Avi) is the recombinant human-derived CD64 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of CD64 Protein, Human (Biotinylated, 272a.a, HEK293, His-Avi) is 272 a.a., with molecular weight of 34-68 kDa.

CD64 protein is a high affinity receptor for the Fc region of immunoglobulins gamma. It plays a role in both innate and adaptive immune responses. CD64 mediates IgG effector functions on monocytes, triggering antibody-dependent cellular cytotoxicity (ADCC) against virus-infected cells. It interacts with IGHG1 and forms a functional signaling complex with FCERG1. CD64 also interacts with FLNA, preventing degradation of FCGR1A. Additionally, it interacts with EPB41L2, LAT, PPL, HCK, and LYN, contributing to its diverse functions in immune regulation.

1.Immobilized Anti-CD20(Ro) Antibody (Rituximab) at 1 μg/mL (100 μL/well) can bind Recombinant Human CD64 / FCGR1A Protein (His & AVI Tag), Biotinylated, the EC₅₀ is 1-3 ng/mL.
2.Biotinylated Human Fc gamma RI, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 5 nM as determined in SPR assay (Biacore T200).

Human

HEK293

C-Avi;C-His

P12314 (Q16-T287)

2209  [NCBI]

MWFLTTLLLWVPVDGQVDTTKAviTLQPPWVSVFQEETVTLHCEVLHLPGSSSTQWFLNGTATQTSTPSYRITSASVNDSGEYRCQRGLSGRSDPIQLEIHRGWLLLQVSSRVFTEGEPLALRCHAWKDKLVYNVLYYRNGKAFKFFHWNSNLTILKTNISHNGTYHCSGMGKHRYTSAGISVTVKELFPAPVLNASVTSPLLEGNLVTLSCETKLLLQRPGLQLYFSFYMGSKTLRGRNTSSEYQILTARREDSGLYWCEAATEDGNVLKRSPELELQVLGLQLPT

34-68 kDa

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.