CDKN1A Protein, Human (GST)

The CDKN1A protein critically controls cell cycle progression and mediates DNA damage-induced G2 arrest through p53/TP53-dependent and CREB3L1-mediated p53-independent pathways. It inhibits cyclin-dependent kinase activity, blocking cell cycle progression by preventing phosphorylation of key substrates. CDKN1A Protein, Human (GST) is the recombinant human-derived CDKN1A protein, expressed by E. coli , with N-GST labeled tag. The total length of CDKN1A Protein, Human (GST) is 163 a.a., with molecular weight of 45 kDa.

The CDKN1A protein assumes a crucial role in controlling cell cycle progression and orchestrating DNA damage-induced G2 arrest. It actively participates in p53/TP53-mediated inhibition of cellular proliferation in response to DNA damage and is also implicated in p53-independent DNA damage-induced G2 arrest, mediated by CREB3L1 in astrocytes and osteoblasts. Functionally, CDKN1A binds to and inhibits cyclin-dependent kinase activity, preventing the phosphorylation of critical cyclin-dependent kinase substrates and thereby blocking cell cycle progression. It contributes to the nuclear localization and assembly of the cyclin D-CDK4 complex, promoting its kinase activity towards RB1. At higher stoichiometric ratios, CDKN1A inhibits the kinase activity of the cyclin D-CDK4 complex and competes with POLD3 for PCNA binding, leading to the inhibition of DNA synthesis by DNA polymerase delta. The intricate regulatory network of CDKN1A includes interactions with HDAC1, MKRN1, PSMA3, CDK4, CDK2, PIM1, STK11, NUAK1, and TRIM39 isoforms, reflecting its diverse role in cell cycle regulation and DNA damage response.

Human

E. coli

N-GST

P38936 (S2-P164)

1026  [NCBI]

SEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP

53 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.